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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0404 [new locus tag: SA_RS02310 ]
  • pan locus tag?: SAUPAN002145000
  • symbol: lpl8
  • pan gene symbol?: lpl8
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA0404 [new locus tag: SA_RS02310 ]
  • symbol: lpl8
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 463725..464507
  • length: 783
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAGTCTATAAAAAGGATTGGATTGTGCATAAGTCTGTTGATTTTAATCATTTTTGCA
    ACATCTTGTGGTAGTAATAAGATTACTGGAGACTCAAAAGAAGAACAAATCAAAAAGAGT
    TTTGCGAAATCTTTAGACAAGTATCCTACTGAAAATCTAGAAGAGTTTTATGATAAAGAA
    GGATATAGAGATGGAGAATTTGAAAAAGATGATAAAGGGACTTGGCTAATTAGATCTGAA
    ATGAAAATCCAATTAAAAGGAGAAAATCTGGAATCTAGGGGAGCAGTTATAGAAATTAAC
    AGAAATACTAGAACGGCTAAAGGGAATTATATTGTTAGAGAAGTTGTTGAAGATAGCGAT
    GGAATGACACACAATCATACAAAAAGGTATCCTGTGAAAATGGAAAATAATAAAATGATT
    CCATTAAAATCAATTGATGATGAAAAAGTAAAAAAAGAAATTGAAGAATTTAAATTCTTT
    GTACAATACGGGAATTTCAAAGAATTGGAAAACTATAAAGAGGACGAAGTGTCATATAAC
    CCAGAAGTACCAATATACTCTGCACAATATCAATTGAAAAACAGTGATTATAATGTAGAG
    CAATTACGTAAGCGATATAATATCCCGACGCAAAAAGCGCCCAAATTATTATTGAAAGGC
    TCAGGTAATTTAAAAGGTTCATCAGTCGGATATAAAAATATTGAATTTACCTTTGTTGAA
    AATAAGGAAGAAAATATTTACTTCACAGATAGTGTCTACTTTAATCCAAGCGAGGATAAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    783

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA0404 [new locus tag: SA_RS02310 ]
  • symbol: Lpl8
  • description: hypothetical protein
  • length: 260
  • theoretical pI: 6.57845
  • theoretical MW: 30311.1
  • GRAVY: -0.856154

Function[edit | edit source]

  • TIGRFAM:
    Staphylococcus tandem lipoproteins (TIGR01742; HMM-score: 347.8)
  • TheSEED  :
    • FIG01107840: hypothetical protein
  • PFAM:
    LolA_LolB (CL0048) DUF576; Csa1 family (PF04507; HMM-score: 417.5)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 4.6
    • Cellwall Score: 2.48
    • Extracellular Score: 2.92
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 5
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide LIPO(Sec/SPII) length 22 aa
    • SP(Sec/SPI): 0.00094
    • TAT(Tat/SPI): 0.000042
    • LIPO(Sec/SPII): 0.998875
    • Cleavage Site: CS pos: 22-23. ATS-CG. Pr: 0.9990
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKSIKRIGLCISLLILIIFATSCGSNKITGDSKEEQIKKSFAKSLDKYPTENLEEFYDKEGYRDGEFEKDDKGTWLIRSEMKIQLKGENLESRGAVIEINRNTRTAKGNYIVREVVEDSDGMTHNHTKRYPVKMENNKMIPLKSIDDEKVKKEIEEFKFFVQYGNFKELENYKEDEVSYNPEVPIYSAQYQLKNSDYNVEQLRKRYNIPTQKAPKLLLKGSGNLKGSSVGYKNIEFTFVENKEENIYFTDSVYFNPSEDK

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]