PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0515 [new locus tag: SA_RS03005 ]
• pan locus tag^?: SAUPAN002330000
• symbol: SA0515
• pan gene symbol^?: dgk
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0515 [new locus tag: SA_RS03005 ]
• symbol: SA0515
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 602028..602645
• length: 618
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123320 NCBI
• RefSeq: NP_373768 NCBI
• BioCyc: see SA_RS03005
• MicrobesOnline: 102794 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAACAAACCTTTTATAGCAATTGAAGGTCCTATTGGCGTAGGTAAATCTTCACTTGCA
  CACAAATTAAGTCAAACTTTAGATTTTTATGAAGAAAAAGAAATCATCACAGAAAATCCA
  TTTTTATCAGACTTTTATGAAGATATCTCTAAATGGAGTTTTCAAACTGAAATGTTCTTT
  TTATGCAATAGATATAAGCAATTTCAAGATGTAACACAACTAAATCAAGGTGTAGTTAGT
  GATTATCATATACATAAAAATAAGATATTTGCTAAAAATACTTTGAGTCCTGTTGAATTT
  CAGAAATTCAGTAAAATTTATGATATTTTAACTGAAGATATGATTATGCCGAATATGATT
  ATCTTTTTAGATGCAGACCTTGATGTGTTAAAATCTAGAATTGCTAAACGTAACCGTAGT
  TTTGAGCATCAAATAGAAGATGAATATCTGTTAAAGTTAAAAAAAGATTATCGTGAGTAT
  TATGAGTCCTTACAAAGTAATGGTTCAAATGTAGTTTTAATCGATACAACTTCTATTGAT
  TTTCTTAAAAATGAACAAGATTACGAAGATATATTACATATTATATTACCTATGATAGGA
  GATATTACCAATGAATAA
  60
  120
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  618
  

⊟Protein

⊟General

• locus tag: SA0515 [new locus tag: SA_RS03005 ]
• symbol: SA0515
• description: hypothetical protein
• length: 205
• theoretical pI: 4.5954
• theoretical MW: 24163.3
• GRAVY: -0.380976

⊟Function

• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions dTMP kinase (TIGR00041; EC 2.7.4.9; HMM-score: 42.9)
  and 5 more
  putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 25)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions cytidylate kinase (TIGR00017; EC 2.7.4.14; HMM-score: 20.4)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 16)
  Metabolism Central intermediary metabolism Phosphorus compounds phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN (TIGR02322; HMM-score: 15.7)
  carbohydrate kinase, thermoresistant glucokinase family (TIGR01313; EC 2.7.1.-; HMM-score: 12.3)
• TheSEED  :
  • Deoxyguanosine kinase (EC 2.7.1.113)
  Nucleosides and Nucleotides Purines Purine conversions  Deoxyadenosine kinase (EC 2.7.1.76)
  and 1 more
  Nucleosides and Nucleotides Purines Purine conversions  Deoxyguanosine kinase (EC 2.7.1.113)
• PFAM:
  P-loop_NTPase (CL0023) dNK; Deoxynucleoside kinase (PF01712; HMM-score: 157.1)
  and 16 more
  Thymidylate_kin; Thymidylate kinase (PF02223; HMM-score: 36.3)
  AAA_18; AAA domain (PF13238; HMM-score: 34.6)
  AAA_33; AAA domain (PF13671; HMM-score: 28.7)
  Cytidylate_kin; Cytidylate kinase (PF02224; HMM-score: 18.7)
  SKI; Shikimate kinase (PF01202; HMM-score: 17.9)
  AAA_22; AAA domain (PF13401; HMM-score: 17.7)
  AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 17.4)
  AAA_17; AAA domain (PF13207; HMM-score: 15.2)
  RNA_helicase; RNA helicase (PF00910; HMM-score: 15.1)
  AAA_14; AAA domain (PF13173; HMM-score: 14.6)
  ATP_bind_2; P-loop ATPase protein family (PF03668; HMM-score: 14.1)
  ABC_tran; ABC transporter (PF00005; HMM-score: 13.8)
  AAA_28; AAA domain (PF13521; HMM-score: 13.6)
  NACHT; NACHT domain (PF05729; HMM-score: 12.8)
  MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 12.7)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006391
  • TAT(Tat/SPI): 0.000277
  • LIPO(Sec/SPII): 0.000894
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926235 NCBI
• RefSeq: NP_373768 NCBI
• UniProt: A0A0H3JT29 UniProt

⊟Protein sequence

• MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSDFYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSPVEFQKFSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREYYESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0514 < SA0515

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications