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NCBI: 06-JUL-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_0519 [new locus tag: NWMN_RS03010 ]
  • pan locus tag?: SAUPAN002330000
  • symbol: NWMN_0519
  • pan gene symbol?: dgk
  • synonym:
  • product: deoxynucleoside kinase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_0519 [new locus tag: NWMN_RS03010 ]
  • symbol: NWMN_0519
  • product: deoxynucleoside kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 598481..599143
  • length: 663
  • essential: unknown other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGTACAATACTCAATGTCTATTTTAACATTTGGAGATGTGAATATGAACAAACCTTTT
    ATAGCAATTGAAGGTCCTATTGGCGTAGGTAAATCTTCACTTGCACACAAATTAAGTCAA
    ACTTTAGATTTTTATGAAGAAAAAGAAATCATCACAGAAAATCCATTTTTATCAGACTTT
    TATGAAGATATCTCTAAATGGAGTTTTCAAACTGAAATGTTCTTTTTATGCAATAGATAT
    AAGCAATTTCAAGATGTAACACAACTAAATCAAGGTGTAGTTAGTGATTATCATATACAT
    AAAAATAAGATATTTGCTAAAAATACTTTGAGTTCTGTTGAATTTCAGAAATTCAGTAAA
    ATTTATGATATTTTAACTGAAGATATGATTATGCCGAATATGATTATCTTTTTAGATGCA
    GACCTTGATGTGTTAAAATCTAGAATTGCTAAACGTAACCGTAGTTTTGAGCATCAAATA
    GAAGATGAATATCTGTTAAAGTTAAAAAAAGATTATCGTGAGTATTATGAGTCCTTACAA
    AGTAATGGTTCAAATGTAGTTTTAATCGATACAACTTCTATTGATTTTCTTAAAAATGAA
    CAAGATTACGAAGATATATTACATATTATATTACCTATGATAGGAGATATTACCAATGAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    663

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: NWMN_0519 [new locus tag: NWMN_RS03010 ]
  • symbol: NWMN_0519
  • description: deoxynucleoside kinase
  • length: 220
  • theoretical pI: 4.5352
  • theoretical MW: 25840.2
  • GRAVY: -0.311364

Function[edit | edit source]

  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions dTMP kinase (TIGR00041; EC 2.7.4.9; HMM-score: 43.8)
    and 5 more
    putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 24.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions cytidylate kinase (TIGR00017; EC 2.7.4.14; HMM-score: 20.2)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 16.2)
    Metabolism Central intermediary metabolism Phosphorus compounds phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN (TIGR02322; HMM-score: 15.5)
    carbohydrate kinase, thermoresistant glucokinase family (TIGR01313; EC 2.7.1.-; HMM-score: 12.1)
  • TheSEED  :
    • Deoxyguanosine kinase (EC 2.7.1.113)
    Nucleosides and Nucleotides Purines Purine conversions  Deoxyadenosine kinase (EC 2.7.1.76)
    and 1 more
    Nucleosides and Nucleotides Purines Purine conversions  Deoxyguanosine kinase (EC 2.7.1.113)
  • PFAM:
    P-loop_NTPase (CL0023) dNK; Deoxynucleoside kinase (PF01712; HMM-score: 154.8)
    and 22 more
    Thymidylate_kin; Thymidylate kinase (PF02223; HMM-score: 39.7)
    AAA_18; AAA domain (PF13238; HMM-score: 27.7)
    AAA_33; AAA domain (PF13671; HMM-score: 26.4)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 18.9)
    Cytidylate_kin; Cytidylate kinase (PF02224; HMM-score: 18.5)
    KAP_NTPase; KAP family P-loop domain (PF07693; HMM-score: 17.3)
    SKI; Shikimate kinase (PF01202; HMM-score: 16.6)
    AAA_22; AAA domain (PF13401; HMM-score: 16.1)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 15.6)
    RapZ-like_N; RapZ-like N-terminal domain (PF03668; HMM-score: 15.6)
    AAA_17; AAA domain (PF13207; HMM-score: 15.3)
    AAA_14; AAA domain (PF13173; HMM-score: 14.7)
    MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 13.5)
    AAA_28; AAA domain (PF13521; HMM-score: 13.4)
    NACHT; NACHT domain (PF05729; HMM-score: 13.3)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.1)
    ABC_tran; ABC transporter (PF00005; HMM-score: 12.9)
    NPHP3_N; Nephrocystin 3, N-terminal (PF24883; HMM-score: 12.9)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 12.8)
    no clan defined PET117; PET assembly of cytochrome c oxidase, mitochondrial (PF15786; HMM-score: 12.5)
    P-loop_NTPase (CL0023) AAA_23; AAA domain (PF13476; HMM-score: 12.4)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 11.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.957
    • Cytoplasmic Membrane Score: 0.0122
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0307
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011186
    • TAT(Tat/SPI): 0.000238
    • LIPO(Sec/SPII): 0.000898
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MVQYSMSILTFGDVNMNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSDFYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQKFSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREYYESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]