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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0604 [new locus tag: SACOL_RS03125 ]
  • pan locus tag?: SAUPAN002330000
  • symbol: SACOL0604
  • pan gene symbol?: dgk
  • synonym:
  • product: deoxynucleoside kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0604 [new locus tag: SACOL_RS03125 ]
  • symbol: SACOL0604
  • product: deoxynucleoside kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 629392..630009
  • length: 618
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAACAAACCTTTTATAGCAATTGAAGGTCCTATTGGCGTAGGTAAATCTTCACTTGCA
    CACAAATTAAGTCAAACTTTAGATTTTTATGAAGAAAAAGAAATCATCACAGAAAATCCA
    TTTTTATCAGACTTTTATGAAGATATCTCTAAATGGAGTTTTCAAACTGAAATGTTCTTT
    TTATGCAATAGATATAAGCAATTTCAAGATGTAACACAACTAAATCAAGGTGTAGTTAGT
    GATTATCATATACATAAAAATAAGATATTTGCTAAAAATACTTTGAGTTCTGTTGAATTT
    CAGAAATTCAGTAAAATTTATGATATTTTAACTGAAGATATGATTATGCCGAATATGATT
    ATCTTTTTAGATGCAGACCTTGATGTGTTAAAATCTAGAATTGCTAAACGTAACCGTAGT
    TTTGAGCATCAAATAGAAGATGAATATCTGTTAAAGTTAAAAAAAGATTATCGTGAGTAT
    TATGAGTCCTTACAAAGTAATGGTTCAAATGTAGTTTTAATCGATACAACTTCTATTGAT
    TTTCTTAAAAATGAACAAGATTACGAAGATATATTACATATTATATTACCTATGATAGGA
    GATATTACCAATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    618

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0604 [new locus tag: SACOL_RS03125 ]
  • symbol: SACOL0604
  • description: deoxynucleoside kinase
  • length: 205
  • theoretical pI: 4.5954
  • theoretical MW: 24153.3
  • GRAVY: -0.377073

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions dTMP kinase (TIGR00041; EC 2.7.4.9; HMM-score: 44)
    and 5 more
    putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 25.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions cytidylate kinase (TIGR00017; EC 2.7.4.14; HMM-score: 20.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 16.4)
    Metabolism Central intermediary metabolism Phosphorus compounds phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN (TIGR02322; HMM-score: 15.8)
    carbohydrate kinase, thermoresistant glucokinase family (TIGR01313; EC 2.7.1.-; HMM-score: 12.4)
  • TheSEED  :
    • Deoxyguanosine kinase (EC 2.7.1.113)
    Nucleosides and Nucleotides Purines Purine conversions  Deoxyadenosine kinase (EC 2.7.1.76)
    and 1 more
    Nucleosides and Nucleotides Purines Purine conversions  Deoxyguanosine kinase (EC 2.7.1.113)
  • PFAM:
    P-loop_NTPase (CL0023) dNK; Deoxynucleoside kinase (PF01712; HMM-score: 155.8)
    and 16 more
    Thymidylate_kin; Thymidylate kinase (PF02223; HMM-score: 39.9)
    AAA_18; AAA domain (PF13238; HMM-score: 34.5)
    AAA_33; AAA domain (PF13671; HMM-score: 31)
    Cytidylate_kin; Cytidylate kinase (PF02224; HMM-score: 18.7)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 17.9)
    SKI; Shikimate kinase (PF01202; HMM-score: 17.9)
    AAA_22; AAA domain (PF13401; HMM-score: 17.9)
    AAA_17; AAA domain (PF13207; HMM-score: 15.6)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 15.3)
    AAA_14; AAA domain (PF13173; HMM-score: 14.8)
    ABC_tran; ABC transporter (PF00005; HMM-score: 14.2)
    AAA_28; AAA domain (PF13521; HMM-score: 13.5)
    MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 12.8)
    NACHT; NACHT domain (PF05729; HMM-score: 12.8)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 12.4)
    PreATP-grasp (CL0483) GSH_synth_ATP; Eukaryotic glutathione synthase, ATP binding domain (PF03917; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006391
    • TAT(Tat/SPI): 0.000277
    • LIPO(Sec/SPII): 0.000894
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSDFYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQKFSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREYYESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 38 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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