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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0770 [new locus tag: SA_RS04400 ]
  • pan locus tag?: SAUPAN002839000
  • symbol: SA0770
  • pan gene symbol?: metP1
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0770 [new locus tag: SA_RS04400 ]
  • symbol: SA0770
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 876616..877311
  • length: 696
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGGGTAAATCATTTAGTGAAATTATAAATGAAATGATTACAATGCCTAATATTCAGTGG
    CCAGAAGTTTGGACTGCAATAGTCGAAACACTATACATGACAGTCGTCTCAACTATATTT
    GCATTTATACTCGGTCTTATTTTAGGTGTGTTATTATTCTTGTCTGCTAAAGGTAAGTCT
    ATCGGTGCAAGGTTATTTTATTCTATCGTTTCTTTCATTGTTAACTTATTTAGAGCGATA
    CCATTTATTATTTTAATTTTATTATTAATTCCATTTACAAGTTTGATACTTGGAACGATA
    AGTGGTCCGACAGGTGCGTTACCAGCCTTGATTATTGGCGCAGCACCGTTTTATGCAAGG
    CTCGTAGAAATTGCTTTTAAAGAAATTGATAAAGGTGTCATCGAAGCGGCCTGGTCAATG
    GGTGCCAATACTTGGATAGTAATTCGTAAAGTCCTCTTACCTGAAGCCATGCCAGCGCTA
    GTGTCTGGCATTACAGTTACAGCAATCGCTTTAGTTGGTTCAACAGCAGTTGCAGGTGTA
    ATTGGTGCCGGTGGTTTAGGAAATTTAGCATACTTAACAGGTTTCACTCGAAATCAAAAT
    GATGTCATTTTAGTATCAACTGTTTTTATTTTAATTATTGTATTTATAATCCAATTCATT
    GGGGATTGGCTTACAAATAAACTTGATAAACGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    696

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0770 [new locus tag: SA_RS04400 ]
  • symbol: SA0770
  • description: hypothetical protein
  • length: 231
  • theoretical pI: 9.46842
  • theoretical MW: 24902.8
  • GRAVY: 1.09221

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 84.6)
    and 15 more
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 51.9)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 46.4)
    2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 37)
    Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 34.8)
    Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein (TIGR00969; HMM-score: 34.3)
    2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 32.4)
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 26.4)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuD (TIGR03003; HMM-score: 26.3)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 24.6)
    Metabolism Transport and binding proteins Amino acids, peptides and amines amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family (TIGR01726; HMM-score: 23.9)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 22.6)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 19)
    Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysW (TIGR02140; HMM-score: 12.6)
    Metabolism Energy metabolism Electron transport cytochrome aa3 quinol oxidase, subunit II (TIGR01432; EC 1.10.3.-; HMM-score: 9.4)
    Metabolism Energy metabolism Electron transport caa(3)-type oxidase, subunit IV (TIGR02229; HMM-score: 7)
  • TheSEED  :
    • Methionine ABC transporter, permease protein MetI
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Methionine ABC transporter permease protein
    and 1 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Degradation  Methionine ABC transporter permease protein
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 75.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 5
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009004
    • TAT(Tat/SPI): 0.000133
    • LIPO(Sec/SPII): 0.001426
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFILGLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISGPTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWIVIRKVLLPEAMPALVSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQFIGDWLTNKLDKR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: S-box (transcription termination) regulon, CodY (repression) regulon
    S-box(RNA)important in Methionine biosynthesis; RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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