PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0784 [new locus tag: SA_RS04480 ]
• pan locus tag^?: SAUPAN003021000
• symbol: SA0784
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0784 [new locus tag: SA_RS04480 ]
• symbol: SA0784
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 890867..892186
• length: 1320
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123599 NCBI
• RefSeq: NP_374045 NCBI
• BioCyc: see SA_RS04480
• MicrobesOnline: 103071 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  121
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  301
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  481
  541
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  661
  721
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  841
  901
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  1021
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  1141
  1201
  1261
  
  TTGAGGCTTACAATTTATCATACGAACGATATTCATAGTCATTTACATGAATACGAACGC
  ATTAAAGTATATATGGCAGAACAACGGCCACGACTTAATCATCCTTCTTTATATGTTGAT
  CTAGGTGATCATGTAGATTTATCCGCACCTATAACTGAAGCAACTTTAGGTAAAAAGAAT
  GTGGCATTACTAAATGAAGCAAAATGTGATGTTGCAACAATCGGTAATAATGAAGGGATG
  ACCATTTCACACGAAGCTTTAAATCACCTTTACGACGAAGCAAAATTTATAGTGACATGT
  AGCAATGTTATAGATGAATCAGGTCATTTACCAAATAATATCGTTTCTTCTTATATTAAG
  GATATGGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTAT
  CGTGCACTAGATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTAAACAT
  CAACGAGGTAAATTTGATGTAATAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAA
  ACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTAT
  TTTGAACATGGTGAGATCAATAATGGTGTACTGATGGCGGCAGCTGGAAAGTATGGTAAT
  TATCTTGGAGAGGTTAATTTAACTTTTGAGGCACATAAAGTAGTACATAAAACTGCAAAG
  ATTATTCCTTTAGAAACATTACCTGAAGTTGAAACTTCATTTGAAGAAGAAGGAAAAACG
  TTAATGTCCAATTCAGTAATTCAACATCCAGTAGTGCTTAAGCGTAGTATGAATCACATA
  ACTGAAGCTGCATACTTATTAGCTCAAAGTGTTTGTGAGTATACACATGCACAATGTGCC
  ATCATCAATGCTGGCTTACTCGTTAAAGATATTGTAAAAGATGAAGTGACAGAATATGAC
  ATTCATCAAATGTTACCGCATCCGATTAATATGGTAAGGGTTAGACTTTCTGGTGTGAAA
  TTAAAAGAGATTATAACTAAAAGTAATAAACAAGAATATATGTATGAACATGCACAAGGT
  TTGGGTTTCAGAGGGAATATATTTGGAGGATATATTCTTTATAATTTAGGGTACATTCAT
  TCTACAGGGCGTTACTATCTGAATGGAGAAGAAATCGAAGACGACAAAGAATATGTATTA
  GGTACGATAGACATGTATACGTTCGGTCGTTATTTCCCAACACTGAAAGAATTACCAAAA
  GAGTATTTAATGCCAGAGTTTTTAAGAGATATATTTAAAGAAAAATTATTGGAATATTAA
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⊟Protein

⊟General

• locus tag: SA0784 [new locus tag: SA_RS04480 ]
• symbol: SA0784
• description: hypothetical protein
• length: 439
• theoretical pI: 5.70465
• theoretical MW: 50144
• GRAVY: -0.191572

⊟Function

• TIGRFAM:
  thiosulfohydrolase SoxB (TIGR04486; HMM-score: 68.7)
  and 2 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 41.8)
  Metabolism Transport and binding proteins Other NAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 41.8)
• TheSEED  :
  • 5'-nucleotidase family protein in cluster with NagD-like phosphatase
  Nucleosides and Nucleotides Purines Purine conversions  5'-nucleotidase family protein in cluster with NagD-like phosphatase
• PFAM:
  Calcineurin (CL0163) Metallophos; Calcineurin-like phosphoesterase (PF00149; HMM-score: 42.3)
  and 2 more
  no clan defined 5_nucleotid_C; 5'-nucleotidase, C-terminal domain (PF02872; HMM-score: 33.6)
  Calcineurin (CL0163) PGA_cap; Bacterial capsule synthesis protein PGA_cap (PF09587; HMM-score: 15.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0.11
  • Cytoplasmic Membrane Score: 0.02
  • Cellwall Score: 8.97
  • Extracellular Score: 0.9
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006663
  • TAT(Tat/SPI): 0.000149
  • LIPO(Sec/SPII): 0.000913
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926512 NCBI
• RefSeq: NP_374045 NCBI
• UniProt: A0A0H3JTJ4 UniProt

⊟Protein sequence

• MRLTIYHTNDIHSHLHEYERIKVYMAEQRPRLNHPSLYVDLGDHVDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSNVIDESGHLPNNIVSSYIKDMDGVKILFVAATAPFTPFYRALDWIVTDPLESIKEEIKHQRGKFDVIIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKYGNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRSMNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRVRLSGVKLKEIITKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEIEDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA0782 > SA0783 > SA0784 > lipA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications