PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0828 [new locus tag: SAUSA300_RS04470 ]
• pan locus tag^?: SAUPAN003021000
• symbol: SAUSA300_0828
• pan gene symbol^?: —
• synonym:
• product: 5'-nucleotidase family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0828 [new locus tag: SAUSA300_RS04470 ]
• symbol: SAUSA300_0828
• product: 5'-nucleotidase family protein
• replicon: chromosome
• strand: +
• coordinates: 908351..909670
• length: 1320
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915322 NCBI
• RefSeq: YP_493528 NCBI
• BioCyc: see SAUSA300_RS04470
• MicrobesOnline: 1292343 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1021
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  TTGAGGCTTACAATTTATCATACGAACGATATTCATAGTCATTTACATGAATACGAACGC
  ATTAAAGCATATATGGCAGAACATCGGCCACGACTTAATCATCCTTCTTTATATGTTGAT
  CTAGGTGATCATGTAGATTTATCCGCACCTATAACTGAAGCAACTTTAGGTAAAAAGAAT
  GTGGCATTACTAAATGAAGCAAAATGTGATGTTGCAACAATCGGTAATAATGAAGGGATG
  ACCATTTCACACGAAGCTTTAAATCACCTTTACGACGAAGCAAAATTTATAGTGACATGT
  AGCAATGTTATAGATGAATCAGGTCATTTACCAAATAATATCGTTTCTTCTTATATTAAG
  GACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTAT
  CGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTT
  CAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAA
  ACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTAT
  TTTGAACATGGTGAAATCAATAATGGTGTACTGATGGCGGCAGCTGGAAAGTATGGTAAT
  TATCTTGGAGAGGTTAATTTAACTTTTGAGGCACATAAAGTAGTACATAAAACTGCAAAG
  ATTATTCCTTTAGAAACATTACCTGAAGTTGAAACTTCATTTGAAGAAGAAGGAAAAACG
  TTAATGTCCAATTCAGTAATTCAACATCCAGTAGTGCTTAAGCGTAGTATGAATCACATA
  ACTGAAGCTGCATACTTATTAGCTCAAAGTGTTTGTGAGTATACACATGCACAATGTGCC
  ATCATCAATGCTGGCTTACTCGTTAAAGATATTGTAAAAGATGAAGTGACAGAATATGAC
  ATTCATCAAATGTTACCGCATCCGATTAATATGGTAAGGGTTAGACTTTTTGGTGTGAAA
  TTAAAAGAGATTATAGCTAAAAGTAATAAACAAGAATATATGTATGAACATGCACAAGGT
  TTGGGTTTCAGAGGGAATATATTTGGAGGATATATTCTTTATAATTTAGGGTACATTCAT
  TCTACAGGGCGTTACTATCTGAATGGAGAAGAAATCGAAGACGACAAAGAATATGTACTA
  GGTACGATAGATATGTATACGTTCGGTCGTTATTTCCCAACATTGAAAGAATTACCAAAA
  GAGTATTTAATGCCAGAGTTTTTAAGAGATATATTTAAAGAAAAATTATTGGAATATTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0828 [new locus tag: SAUSA300_RS04470 ]
• symbol: SAUSA300_0828
• description: 5'-nucleotidase family protein
• length: 439
• theoretical pI: 5.59947
• theoretical MW: 50113
• GRAVY: -0.161276

⊟Function

• TIGRFAM:
  thiosulfohydrolase SoxB (TIGR04486; HMM-score: 65.5)
  and 2 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 35.2)
  Metabolism Transport and binding proteins Other NAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 35.2)
• TheSEED  :
  • 5'-nucleotidase family protein in cluster with NagD-like phosphatase
  Nucleosides and Nucleotides Purines Purine conversions  5'-nucleotidase family protein in cluster with NagD-like phosphatase
• PFAM:
  Calcineurin (CL0163) Metallophos; Calcineurin-like phosphoesterase (PF00149; HMM-score: 42.5)
  and 2 more
  no clan defined 5_nucleotid_C; 5'-nucleotidase, C-terminal domain (PF02872; HMM-score: 30.7)
  Calcineurin (CL0163) PGA_cap; Bacterial capsule synthesis protein PGA_cap (PF09587; HMM-score: 13.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cellwall
  • Cytoplasmic Score: 0.11
  • Cytoplasmic Membrane Score: 0.02
  • Cellwall Score: 8.97
  • Extracellular Score: 0.9
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007738
  • TAT(Tat/SPI): 0.000201
  • LIPO(Sec/SPII): 0.001555
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162282 NCBI
• RefSeq: YP_493528 NCBI
• UniProt: A0A0H2XKC2 UniProt

⊟Protein sequence

• MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDHVDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSNVIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIELQRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKYGNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRSMNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRVRLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEIEDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0826 > SAUSA300_0827 > SAUSA300_0828

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications