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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS04470 [old locus tag: SAUSA300_0828 ]
  • pan locus tag?: SAUPAN003021000
  • symbol: SAUSA300_RS04470
  • pan gene symbol?: —
  • synonym:
  • product: bifunctional metallophosphatase/5'-nucleotidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS04470 [old locus tag: SAUSA300_0828 ]
  • symbol: SAUSA300_RS04470
  • product: bifunctional metallophosphatase/5'-nucleotidase
  • replicon: chromosome
  • strand: +
  • coordinates: 908351..909670
  • length: 1320
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    TTGAGGCTTACAATTTATCATACGAACGATATTCATAGTCATTTACATGAATACGAACGC
    ATTAAAGCATATATGGCAGAACATCGGCCACGACTTAATCATCCTTCTTTATATGTTGAT
    CTAGGTGATCATGTAGATTTATCCGCACCTATAACTGAAGCAACTTTAGGTAAAAAGAAT
    GTGGCATTACTAAATGAAGCAAAATGTGATGTTGCAACAATCGGTAATAATGAAGGGATG
    ACCATTTCACACGAAGCTTTAAATCACCTTTACGACGAAGCAAAATTTATAGTGACATGT
    AGCAATGTTATAGATGAATCAGGTCATTTACCAAATAATATCGTTTCTTCTTATATTAAG
    GACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTAT
    CGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTT
    CAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAA
    ACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTAT
    TTTGAACATGGTGAAATCAATAATGGTGTACTGATGGCGGCAGCTGGAAAGTATGGTAAT
    TATCTTGGAGAGGTTAATTTAACTTTTGAGGCACATAAAGTAGTACATAAAACTGCAAAG
    ATTATTCCTTTAGAAACATTACCTGAAGTTGAAACTTCATTTGAAGAAGAAGGAAAAACG
    TTAATGTCCAATTCAGTAATTCAACATCCAGTAGTGCTTAAGCGTAGTATGAATCACATA
    ACTGAAGCTGCATACTTATTAGCTCAAAGTGTTTGTGAGTATACACATGCACAATGTGCC
    ATCATCAATGCTGGCTTACTCGTTAAAGATATTGTAAAAGATGAAGTGACAGAATATGAC
    ATTCATCAAATGTTACCGCATCCGATTAATATGGTAAGGGTTAGACTTTTTGGTGTGAAA
    TTAAAAGAGATTATAGCTAAAAGTAATAAACAAGAATATATGTATGAACATGCACAAGGT
    TTGGGTTTCAGAGGGAATATATTTGGAGGATATATTCTTTATAATTTAGGGTACATTCAT
    TCTACAGGGCGTTACTATCTGAATGGAGAAGAAATCGAAGACGACAAAGAATATGTACTA
    GGTACGATAGATATGTATACGTTCGGTCGTTATTTCCCAACATTGAAAGAATTACCAAAA
    GAGTATTTAATGCCAGAGTTTTTAAGAGATATATTTAAAGAAAAATTATTGGAATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS04470 [old locus tag: SAUSA300_0828 ]
  • symbol: SAUSA300_RS04470
  • description: bifunctional metallophosphatase/5'-nucleotidase
  • length: 439
  • theoretical pI: 5.59947
  • theoretical MW: 50113
  • GRAVY: -0.161276

⊟Function[edit | edit source]

  • TIGRFAM:
    thiosulfohydrolase SoxB (TIGR04486; HMM-score: 65.5)
    and 2 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 35.2)
    Metabolism Transport and binding proteins Other NAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 35.2)
  • TheSEED: see SAUSA300_0828
  • PFAM:
    Calcineurin (CL0163) Metallophos; Calcineurin-like phosphoesterase (PF00149; HMM-score: 42.5)
    and 2 more
    no clan defined 5_nucleotid_C; 5'-nucleotidase, C-terminal domain (PF02872; HMM-score: 30.7)
    Calcineurin (CL0163) PGA_cap; Bacterial capsule synthesis protein PGA_cap (PF09587; HMM-score: 13.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.11
    • Cytoplasmic Membrane Score: 0.02
    • Cellwall Score: 8.97
    • Extracellular Score: 0.9
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007738
    • TAT(Tat/SPI): 0.000201
    • LIPO(Sec/SPII): 0.001555
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDHVDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSNVIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIELQRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKYGNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRSMNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRVRLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEIEDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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