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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1065 [new locus tag: SA_RS06040 ]
  • pan locus tag?: SAUPAN003505000
  • symbol: cfxE
  • pan gene symbol?: cfxE
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1065 [new locus tag: SA_RS06040 ]
  • symbol: cfxE
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1205482..1206126
  • length: 645
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACAAAACTATATCCATCATTATTATCTGTTGATTTTTTGGATTTACAACATGAATTA
    AAACGACTTGAAGAAGCAGGTGTCGACGGAGTTCATTTTGATGTTATGGATGGTCAATTT
    GTGCCTAATATATCTATTGGTTTACCAATATTAGATGCAGTAAGAAAAGGCACAACATTA
    CCTATAGACGTACATTTGATGATTGAAAATCCAGAAAAGTATATTGCATCATTTGCAGAA
    CATGGTGCCGATATGATTTCAATTCATGTCGAATCAACGCCTCATATTCATCGTGCTATT
    CAAATGATTAAACATTTAGATAAAAAAGCTGGTGTAGTAATTAATCCTGGTACACCAATA
    TCACAAATTGAACCTATTTTAGACATTGTTGATTATGTATTAGTGATGACAGTTAACCCA
    GGGTTTGGTGGTCAATCATTTATTGATCAATGCGTAGAAAAAATAGCGGGTCTTAATGCT
    ATTAAAATGGAACGTCAATTAAACTTTGATATTGAAGTTGATGGAGGCGTAAATACCGAT
    ACAGCGAAAGTTTGTGTTGAAAATGGTGCTACAATGCTAGTAACAGGTTCATTTTTCTTT
    AAACAAGAAGATTATAAAAAAGTCACACAAGCATTGAAAGGTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    645

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1065 [new locus tag: SA_RS06040 ]
  • symbol: CfxE
  • description: hypothetical protein
  • length: 214
  • theoretical pI: 4.91189
  • theoretical MW: 23572.1
  • GRAVY: 0.0668224

⊟Function[edit | edit source]

  • reaction:
    EC 5.1.3.1?  ExPASy
    Ribulose-phosphate 3-epimerase D-ribulose 5-phosphate = D-xylulose 5-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Pentose phosphate pathway ribulose-phosphate 3-epimerase (TIGR01163; EC 5.1.3.1; HMM-score: 274.9)
    and 2 more
    3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 24.5)
    Metabolism Amino acid biosynthesis Histidine family phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (TIGR02129; EC 5.3.1.16; HMM-score: 13.7)
  • TheSEED  :
    • Ribulose-phosphate 3-epimerase (EC 5.1.3.1)
    Carbohydrates Central carbohydrate metabolism Pentose phosphate pathway  Ribulose-phosphate 3-epimerase (EC 5.1.3.1)
    and 2 more
    Carbohydrates One-carbon Metabolism Formaldehyde assimilation: Ribulose monophosphate pathway  Ribulose-phosphate 3-epimerase (EC 5.1.3.1)
    DNA Metabolism DNA repair 2-phosphoglycolate salvage  Ribulose-phosphate 3-epimerase (EC 5.1.3.1)
  • PFAM:
    TIM_barrel (CL0036) Ribul_P_3_epim; Ribulose-phosphate 3 epimerase family (PF00834; HMM-score: 252.8)
    and 3 more
    His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 14.9)
    QRPTase_C; Quinolinate phosphoribosyl transferase, C-terminal domain (PF01729; HMM-score: 14)
    PBP (CL0177) PBP_like; PBP superfamily domain (PF12727; HMM-score: 13.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: a divalent metal cation
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002483
    • TAT(Tat/SPI): 0.000119
    • LIPO(Sec/SPII): 0.000211
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNISIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQMIKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNAIKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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