PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1064 [new locus tag: SA_RS06035 ]
• pan locus tag^?: SAUPAN003504000
• symbol: SA1064
• pan gene symbol^?: rsgA
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1064 [new locus tag: SA_RS06035 ]
• symbol: SA1064
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1204606..1205481
• length: 876
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123895 NCBI
• RefSeq: NP_374337 NCBI
• BioCyc: see SA_RS06035
• MicrobesOnline: 103363 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  TTGAAGACAGGTCGAATAGTGAAATCAATTAGTGGGGTATATCAAGTAGACGTTAATGGC
  GAACGTTTCAATACAAAACCACGAGGATTATTTAGAAAGAAAAAATTTTCACCGGTAGTT
  GGTGATATAGTGGAATTTGAAGTACAAAACATTAACGAAGGCTATATTCATCAAGTGTTT
  GAGCGGAAAAATGAGTTGAAAAGACCACCTGTAAGTAATATAGATACACTAGTAATTGTA
  ATGAGTGCTGTCGAGCCAAATTTTTCAACGCAATTATTAGATCGATTTTTAGTTATTGCA
  CATTCGTATCAGTTAAATGCGAGAGTTTTGGTGACTAAAAAAGATAAAACACCAATTGAA
  AAGCAGTTCGAAATTAATGAGTTGTTGAAAATATATGAAAATATTGGCTATGAGACTGAA
  TTTATTGGAAATGATGATGATCGAAAAAAAATTGTAGAAGCTTGGCCAGCTGGACTTATA
  GTACTTAGTGGTCAATCAGGTGTCGGTAAGTCCACTTTCTTAAATCATTATCGTCCAGAA
  CTTAATCTTGAGACAAATGATATATCAAAATCATTAAATCGAGGAAAGCATACTACAAGA
  CATGTCGAACTATTCGAACGTCAAAACGGTTATATTGCAGACACACCTGGATTCAGTGCT
  TTAGATTTTGATCATATAGATAAAGATGAAATAAAAGATTATTTTCTTGAATTAAATCGA
  TATGGTGAAACATGTAAGTTTAGGAATTGTAATCATATCAAAGAACCTAATTGTAATGTT
  AAGCATCAATTAGAGATAGGGAATATTGCGCAATTTAGATACGACCATTATTTACAACTA
  TTTAATGAAATTTCAAATAGAAAGGTTAGATATTAA
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⊟Protein

⊟General

• locus tag: SA1064 [new locus tag: SA_RS06035 ]
• symbol: SA1064
• description: hypothetical protein
• length: 291
• theoretical pI: 8.42051
• theoretical MW: 33875.2
• GRAVY: -0.580412

⊟Function

• reaction:
  EC 3.6.1.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein synthesis Translation factors ribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 246.2)
  and 6 more
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 23.8)
  Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 17.1)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 15.4)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 12.7)
  Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 12.3)
  Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 12.3)
• TheSEED  :
  • Ribosome small subunit biogenesis RbfA-release protein RsgA
  Protein Metabolism Protein biosynthesis Universal GTPases  Ribosome small subunit-stimulated GTPase EngC
• PFAM:
  P-loop_NTPase (CL0023) RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 179.5)
  and 14 more
  OB (CL0021) RsgA_N; RsgA N-terminal domain (PF16745; HMM-score: 70)
  P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 25)
  AAA_22; AAA domain (PF13401; HMM-score: 17.3)
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 16.6)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 15.8)
  ABC_tran; ABC transporter (PF00005; HMM-score: 15.1)
  RNA_helicase; RNA helicase (PF00910; HMM-score: 14.5)
  AAA_18; AAA domain (PF13238; HMM-score: 14.3)
  AAA_23; AAA domain (PF13476; HMM-score: 14.3)
  KAP_NTPase; KAP family P-loop domain (PF07693; HMM-score: 13.7)
  AAA_24; AAA domain (PF13479; HMM-score: 13.6)
  AAA_25; AAA domain (PF13481; HMM-score: 13.4)
  AAA_33; AAA domain (PF13671; HMM-score: 13.4)
  T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 11.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006769
  • TAT(Tat/SPI): 0.000325
  • LIPO(Sec/SPII): 0.000999
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926804 NCBI
• RefSeq: NP_374337 NCBI
• UniProt: P67682 UniProt

⊟Protein sequence

• MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIVEFEVQNINEGYIHQVFERKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHSYQLNARVLVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLIVLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGFSALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDHYLQLFNEISNRKVRY

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA1064 > cfxE > SA1066

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications