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⊟Summary[edit | edit source]
- pan ID?: SAUPAN003504000
- symbol?: rsgA
- synonym:
- description?: GTPase
- GTPase
- GTPase engC, putative
- ribosome small subunit-dependent GTPase A
- putative ribosome biogenesis GTPase RsgA
- Ribosome small subunit-stimulated GTPase EngC
- RsgA, truncated ribosome small subunit-dependent GTPase A
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 3844981..3845437
- synteny block?: BlockID0025830
- occurrence?: in 100% of 33 strains
rsgA (yjeQ): 30S ribosome checkpoint protein and ribosome-associated GTPase RsgA [1]
A crystal structure is available: 6ZJO
Ribosome-associated GTPases including Era, RsgA, RbgA, HflX, ObgE, EngA and EngB are widely-conserved, stringent response alarmone-responsive ribosomal assembly cofactors
• RsgA acts as a checkpoint protein to release biogenesis factors (RbfA) from mature 30S ribosomes and block initiation factor binding. Alarmone binding prevents RbfA displacement resulting in immature ribosomes.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1179 (engC)
08BA02176:
C248_1251
11819-97:
MS7_1179 (rsgA)
6850:
RSAU_001103 (engC)
71193:
ST398NM01_1220
ECT-R 2:
ECTR2_1077 (rsgA)
ED133:
SAOV_1224
ED98:
SAAV_1193
HO 5096 0412:
SAEMRSA15_10540
JH1:
SaurJH1_1305
JH9:
SaurJH9_1280
JKD6008:
SAA6008_01176 (rsgA)
JKD6159:
SAA6159_01074 (rsgA)
LGA251:
SARLGA251_11320 (rsgA)
M013:
M013TW_1159
MRSA252:
SAR1197
MSHR1132:
SAMSHR1132_10620
MSSA476:
SAS1155
Mu3:
SAHV_1211
Mu50:
SAV1221
MW2:
MW1104
RF122:
SAB1085
ST398:
SAPIG1220 (rsgA)
T0131:
SAT0131_01270
TCH60:
HMPREF0772_12011 (rsgA)
TW20:
SATW20_12150 (rsgA)
USA300_TCH1516:
USA300HOU_1158 (engC)
VC40:
SAVC_05270 (rsgA)
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIVEFEVQNINEGYIHQVF
N315 MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIVEFEVQNINEGYIHQVF
NCTC8325 MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIVEFEVQNINEGYIHQVF
Newman MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIVEFEVQNINEGYIHQVF
USA300_FPR3757 MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIVEFEVQNINEGYIHQVF
************************************************************
COL ERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHSYQLNARILVTKKDKTPIE
N315 ERKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHSYQLNARVLVTKKDKTPIE
NCTC8325 ERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHSYQLNARILVTKKDKTPIE
Newman ERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHSYQLNARILVTKKDKTPIE
USA300_FPR3757 ERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHSYQLNARILVTKKDKTPIE
**:*********************************************:***********
COL KQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLIVLSGQSGVGKSTFLNHYRPE
N315 KQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLIVLSGQSGVGKSTFLNHYRPE
NCTC8325 KQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLIVLSGQSGVGKSTFLNHYRPE
Newman KQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLIVLSGQSGVGKSTFLNHYRPE
USA300_FPR3757 KQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLIVLSGQSGVGKSTFLNHYRPE
************************************************************
COL LNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGFSALDFDHIDKDEIKDYFLELNR
N315 LNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGFSALDFDHIDKDEIKDYFLELNR
NCTC8325 LNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGFSALDFDHIDKDEIKDYFLELNR
Newman LNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGFSALDFDHIDKDEIKDYFLELNR
USA300_FPR3757 LNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGFSALDFDHIDKDEIKDYFLELNR
************************************************************
COL YGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDHYLQLFNEISNRKVRY
N315 YGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDHYLQLFNEISNRKVRY
NCTC8325 YGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDHYLQLFNEISNRKVRY
Newman YGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDHYLQLFNEISNRKVRY
USA300_FPR3757 YGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDHYLQLFNEISNRKVRY
***************************************************
- ↑ Tracey L Campbell, James Henderson, David E Heinrichs, Eric D Brown
The yjeQ gene is required for virulence of Staphylococcus aureus.
Infect Immun: 2006, 74(8);4918-21
[PubMed:16861682] [WorldCat.org] [DOI] (P p)Ajitha Jeganathan, Aida Razi, Brett Thurlow, Joaquin Ortega
The C-terminal helix in the YjeQ zinc-finger domain catalyzes the release of RbfA during 30S ribosome subunit assembly.
RNA: 2015, 21(6);1203-16
[PubMed:25904134] [WorldCat.org] [DOI] (I p)Rebecca M Corrigan, Lauren E Bellows, Alison Wood, Angelika Gründling
ppGpp negatively impacts ribosome assembly affecting growth and antimicrobial tolerance in Gram-positive bacteria.
Proc Natl Acad Sci U S A: 2016, 113(12);E1710-9
[PubMed:26951678] [WorldCat.org] [DOI] (I p)Brett Thurlow, Joseph H Davis, Vivian Leong, Trevor F Moraes, James R Williamson, Joaquin Ortega
Binding properties of YjeQ (RsgA), RbfA, RimM and Era to assembly intermediates of the 30S subunit.
Nucleic Acids Res: 2016, 44(20);9918-9932
[PubMed:27382067] [WorldCat.org] [DOI] (I p)Jorge Pedro López-Alonso, Tatsuya Kaminishi, Takeshi Kikuchi, Yuya Hirata, Idoia Iturrioz, Neha Dhimole, Andreas Schedlbauer, Yoichi Hase, Simon Goto, Daisuke Kurita, Akira Muto, Shu Zhou, Chieko Naoe, Deryck J Mills, David Gil-Carton, Chie Takemoto, Hyouta Himeno, Paola Fucini, Sean R Connell
RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket.
Nucleic Acids Res: 2017, 45(11);6945-6959
[PubMed:28482099] [WorldCat.org] [DOI] (I p)Daniel J Bennison, Jose A Nakamoto, Timothy D Craggs, Pohl Milón, John B Rafferty, Rebecca M Corrigan
The Stringent Response Inhibits 70S Ribosome Formation in Staphylococcus aureus by Impeding GTPase-Ribosome Interactions.
mBio: 2021, 12(6);e0267921
[PubMed:34749534] [WorldCat.org] [DOI] (I p)