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NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1071 [new locus tag: SA_RS06070 ]
  • pan locus tag?: SAUPAN003512000
  • symbol: SA1071
  • pan gene symbol?: fapR
  • synonym:
  • product: fatty acid biosynthesis transcriptional regulator

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA1071 [new locus tag: SA_RS06070 ]
  • symbol: SA1071
  • product: fatty acid biosynthesis transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 1212289..1212846
  • length: 558
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    TTGAAACTAAAGAAAGATAAACGTAGAGAAGCAATCAGACAACAAATTGATAGCAATCCC
    TTCATCACAGACCATGAACTAAGCGACTTATTTCAAGTGAGTATACAAACAATTCGTTTA
    GATCGCACTTATTTAAACATACCAGAATTAAGGAAGCGTATTAAATTAGTTGCTGAAAAG
    AATTATGACCAAATAAGTTCTATTGAAGAACAAGAATTTATTGGTGATTTGATTCAAGTC
    AATCCAAATGTTAAAGCGCAATCAATTTTAGATATTACATCGGATTCTGTTTTTCATAAA
    ACTGGAATTGCGCGTGGTCATGTGCTGTTTGCTCAGGCAAATTCGTTATGTGTTGCGCTA
    ATTAAGCAACCAACAGTTTTAACTCATGAGAGTAGCATTCAATTTATTGAAAAAGTAAAA
    TTAAATGATACGGTAAGAGCAGAAGCACGAGTTGTAAATCAAACTGCAAAACATTATTAC
    GTCGAAGTAAAGTCATATGTTAAACATACATTAGTTTTCAAAGGAAATTTTAAAATGTTT
    TATGATAAGCGAGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    558

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA1071 [new locus tag: SA_RS06070 ]
  • symbol: SA1071
  • description: fatty acid biosynthesis transcriptional regulator
  • length: 185
  • theoretical pI: 9.99026
  • theoretical MW: 21433.5
  • GRAVY: -0.389189

Function[edit | edit source]

  • TIGRFAM:
    phenylacetic acid degradation protein PaaD (TIGR02286; HMM-score: 19.4)
    and 1 more
    uncharacterized domain 1 (TIGR00369; HMM-score: 12.6)
  • TheSEED  :
    • Transcription factor FapR
  • PFAM:
    HotDog (CL0050) 4HBT; Thioesterase superfamily (PF03061; HMM-score: 24.4)
    MaoC_dehydratas; MaoC like domain (PF01575; HMM-score: 21.1)
    and 3 more
    HTH (CL0123) HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 18.5)
    HTH_11; HTH domain (PF08279; HMM-score: 14.2)
    no clan defined Sigma54_AID; Sigma-54 factor, Activator interacting domain (AID) (PF00309; HMM-score: 12)

Structure, modifications & cofactors[edit | edit source]

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000877
    • TAT(Tat/SPI): 0.000163
    • LIPO(Sec/SPII): 0.000157
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIRLDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSVFHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQTAKHYYVEVKSYVKHTLVFKGNFKMFYDKRG

Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: FapR* (repression) regulon
    FapR*(TF)important in Fatty acid biosynthesis; RegPrecise 

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]