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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01196
  • pan locus tag?: SAUPAN003512000
  • symbol: SAOUHSC_01196
  • pan gene symbol?: fapR
  • synonym:
  • product: fatty acid biosynthesis transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01196
  • symbol: SAOUHSC_01196
  • product: fatty acid biosynthesis transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 1148093..1148665
  • length: 573
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAGGGGTGAGACGTTGAAACTAAAGAAAGATAAACGTAGAGAAGCAATCAGACAACAA
    ATTGATAGCAATCCCTTCATCACAGACCATGAACTAAGCGACTTATTTCAAGTGAGTATA
    CAAACAATTCGTTTAGATCGCACTTATTTAAACATACCAGAATTAAGGAAGCGTATTAAA
    TTAGTTGCTGAAAAGAATTATGACCAAATAAGTTCTATTGAAGAACAAGAATTTATTGGT
    GATTTGATTCAAGTCAATCCAAATGTTAAAGCGCAATCAATTTTAGATATTACATCGGAT
    TCTGTTTTTCATAAAACTGGAATTGCGCGTGGTCATGTGCTGTTTGCTCAGGCAAATTCG
    TTATGTGTTGCGCTAATTAAGCAACCAACAGTTTTAACTCATGAGAGTAGCATTCAATTT
    ATTGAAAAAGTAAAATTAAATGATACGGTAAGAGCAGAAGCACGAGTTGTAAATCAAACT
    GCAAAACATTATTACGTCGAAGTAAAGTCATATGTTAAACATACATTAGTTTTCAAAGGA
    AATTTTAAAATGTTTTATGATAAGCGAGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    573

⊟Protein[edit | edit source]

Protein Data Bank: 4A0X
Protein Data Bank: 4A0Y
Protein Data Bank: 4A0Z
Protein Data Bank: 4A12

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01196
  • symbol: SAOUHSC_01196
  • description: fatty acid biosynthesis transcriptional regulator
  • length: 190
  • theoretical pI: 9.98992
  • theoretical MW: 21990.1
  • GRAVY: -0.406842

⊟Function[edit | edit source]

  • TIGRFAM:
    phenylacetic acid degradation protein PaaD (TIGR02286; HMM-score: 19.3)
    and 1 more
    uncharacterized domain 1 (TIGR00369; HMM-score: 12.6)
  • TheSEED  :
    • Transcription factor FapR
  • PFAM:
    HotDog (CL0050) 4HBT; Thioesterase superfamily (PF03061; HMM-score: 24.4)
    MaoC_dehydratas; MaoC like domain (PF01575; HMM-score: 21.1)
    and 3 more
    HTH (CL0123) HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 18.4)
    HTH_11; HTH domain (PF08279; HMM-score: 14.2)
    no clan defined Sigma54_AID; Sigma-54 factor, Activator interacting domain (AID) (PF00309; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001346
    • TAT(Tat/SPI): 0.000132
    • LIPO(Sec/SPII): 0.000251
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIRLDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSVFHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQTAKHYYVEVKSYVKHTLVFKGNFKMFYDKRG

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: FapR* (repression) regulon
    FapR*(TF)important in Fatty acid biosynthesis; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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