PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1312 [new locus tag: SA_RS07440 ]
• pan locus tag^?: SAUPAN003946000
• symbol: ebpS
• pan gene symbol^?: ebpS
• synonym:
• product: elastin binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1312 [new locus tag: SA_RS07440 ]
• symbol: ebpS
• product: elastin binding protein
• replicon: chromosome
• strand: -
• coordinates: 1518794..1520254
• length: 1461
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124152 NCBI
• RefSeq: NP_374595 NCBI
• BioCyc: see SA_RS07440
• MicrobesOnline: 103621 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  481
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  901
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  1021
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  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGTCTAATAATTTTAAAGATGACTTTGAAAAAAATCGTCAATCGATAGACACAAATTCA
  CATCAAGACCATACGGAAGATGTTGAAAAAGACCAATCAGAATTAGAACATCAGGATACA
  ATAGAGAATACGGAGCAACAGTTTCCGCCAAGAAATGCCCAAAGAAGAAAAAGACGCCGT
  GATTTAGCAACGAATCATAATAAACAAGTTCACAATGAATCACAAACATCTGAAGACAAT
  GTTCAAAATGAGGCTGGCACAATAGATGATCGTCAAGTCGAATCATCACACAGTACTGAA
  AGTCAAGAACCTAGCCATCAAGACAGTACACCTCAACATGAAGAGGAATATTATAATAAG
  AATGCTTTTGCAATGGATAAATCACATCCAGAACCAATCGAAGACAATGATAAACACGAG
  ACTATTAAAGATGCAGAAAATAACACTGAGCATTCAACAGTTTCTGATAAGAGTATAGCT
  GAACAATCTCAGCAACCTAAACCATATTTTGCAACAGGTGCTAACCAAGCAAATACATCA
  AAAGATAAACATGATGATGTAACTGTTAAGCAAGACAAAGATGAATCTAAAGATCATCAT
  AGTGGTAAAAAAGGCGCAGCAATTGGTGCTGGAACAGCGGGTGTTGCAGGTGCAGCTGGT
  GCAATGGGTGTTTCTAAAGCTAAGAAACATTCAAATGACGCTCAAAACAAAAGTAATTCT
  GACAAGTCGAATAACTCGACTGAGGATAAAGCGTCTCAAGATAAGTCTAAAGATCATCAT
  AATGGCAAAAAAGGTGCAGCGATCGGTGCTGGAACAGCAGGTTTGGCTGGAGGCGCAGCA
  AGTAAAAGTGCTTCTGCCGCTTCAAAACCACATGCCTCTAATAATGCAAGCCAAAACCAT
  GATGAACATGACAATCATGACAGAGATAAAGAACGTAAAAAAGGTGGCATGGCCAAAGTA
  TTGTTACCATTAATTGCAGCTGTACTAATTATCGGTGCATTAGCGATATTTGGAGGCATG
  GCATTAAACAATCATAATAATGGTACAAAAGAAAATAAAATCGCGAATACAAATAAAAAT
  AATGCTGATGAAAGTAAAGACAAAGACACATCTAAAGACGCTTCTAAAGATAAATCAAAA
  TCTACAGACAGTGATAAATCAAAAGAGGATCAAGACAAAGCGACTAAAGATGAATCTGAT
  AATGATCAAAACAACGCTAATCAAGCGAACAATCAAGCACAAAATAATCAAAATCAACAA
  CAAGCTAATCAAAATCAACAACAGCAACAACAACGTCAAGGTGGTGGCCAAAGACATACA
  GTGAATGGTCAAGAAAACTTATACCGTATCGCAATTCAATACTACGGTTCAGGTTCACCG
  GAAAATGTTGAAAAAATTAGACGTGCCAATGGTTTAAGTGGTAACAATATTAGAAACGGT
  CAACAAATCGTTATTCCATAA
  60
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  1461
  

⊟Protein

⊟General

• locus tag: SA1312 [new locus tag: SA_RS07440 ]
• symbol: EbpS
• description: elastin binding protein
• length: 486
• theoretical pI: 6.4082
• theoretical MW: 53181.1
• GRAVY: -1.52428

⊟Function

• TIGRFAM:
• TheSEED  :
  • Elastin binding protein EbpS
  Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Elastin binding protein EbpS
• PFAM:
  LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 25.1)
  no clan defined DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 20.2)
  and 3 more
  Tetraspannin (CL0347) DUF4064; Protein of unknown function (DUF4064) (PF13273; HMM-score: 13.3)
  no clan defined DUF2970; Protein of unknown function (DUF2970) (PF11174; HMM-score: 12)
  Glycine-zipper (CL0500) Gly-zipper_OmpA; Glycine-zipper domain (PF13436; HMM-score: 10.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 10
  • Extracellular Score: 0
  • Internal Helix: 1
• LocateP: Intracellular /TMH start AFTER 60
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: Possibly Sec-
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.014107
  • TAT(Tat/SPI): 0.084419
  • LIPO(Sec/SPII): 0.003534
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 15927062 NCBI
• RefSeq: NP_374595 NCBI
• UniProt: Q7A5I6 UniProt

⊟Protein sequence

• MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENTEQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQEPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHETIKDAENNTEHSTVSDKSIAEQSQQPKPYFATGANQANTSKDKHDDVTVKQDKDESKDHHSGKKGAAIGAGTAGVAGAAGAMGVSKAKKHSNDAQNKSNSDKSNNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLAGGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGALAIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQDKATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRIAIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

T J Foster, M Höök
Surface protein adhesins of Staphylococcus aureus.
Trends Microbiol: 1998, 6(12);484-8
[PubMed:10036727] [WorldCat.org] [DOI] (P p)