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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1522 [new locus tag: SACOL_RS07760 ]
  • pan locus tag?: SAUPAN003946000
  • symbol: SACOL1522
  • pan gene symbol?: ebpS
  • synonym:
  • product: elastin binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1522 [new locus tag: SACOL_RS07760 ]
  • symbol: SACOL1522
  • product: elastin binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1560353..1561813
  • length: 1461
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTCTAATAATTTTAAAGATGACTTTGAAAAAAATCGTCAATCGATAGACACAAATTCA
    CATCAAGACCATACGGAAGATGTTGAAAAAGACCAATCAGAATTAGAACATCAGGATACA
    ATAGAGAATACGGAGCAACAGTTTCCGCCAAGAAATGCCCAAAGAAGAAAAAGACGCCGT
    GATTTAGCAACGAATCATAATAAACAAGTTCACAATGAATCACAAACATCTGAAGACAAT
    GTTCAAAATGAGGCTGGCACAATAGATGATCGTCAAGTCGAATCATCACACAGTACTGAA
    AGTCAAGAACCTAGCCATCAAGACAGTACACCTCAACATGAAGAGGAATATTATAATAAG
    AATGCTTTTGCAATGGATAAATCACATCCAGAACCAATCGAAGACAATGATAAACACGAT
    ACTATTAAAAATGCAGAAAATAACACTGAGCATTCAACAGTTTCTGATAAGAGTGAAGCT
    GAACAATCTCAGCAACCTAAACCATATTTTACAACAGGTGCTAACCAATCAGAAACATCA
    AAAAATGAACATGATAATGATTCTGTAAAACAAGATCAAGATGAACCTAAAGAACATCAT
    AATGGTAAAAAAGCAGCAGCTATTGGTGCTGGAACAGCAGGTGTTGCAGGTGCAGCTGGT
    GCAATGGCTGCTTCTAAAGCTAAGAAACATTCAAATGACGCTCAAAACAAAAGTAATTCT
    GGCAAGGCGAATAACTCGACTGAGGATAAAGCGTCTCAAGATAAGTCTAAAGATCATCAT
    AATGGCAAAAAAGGTGCAGCGATCGGTGCTGGAACAGCAGGTTTGGCTGGAGGCGCAGCA
    AGTAAAAGTGCTTCTGCCGCTTCAAAACCACATGCCTCTAATAATGCAAGCCAAAACCAT
    GATGAACATGACAATCATGACAGAGATAAAGAACGTAAAAAAGGTGGCATGGCCAAAGTA
    TTGTTACCATTAATTGCAGCTGTACTAATTATCGGTGCATTAGCGATATTTGGAGGCATG
    GCATTAAACAATCATAATAATGGTACAAAAGAAAATAAAATCGCGAATACAAATAAAAAT
    AATGCTGATGAAAGTAAAGACAAAGACACATCTAAAGACGCTTCTAAAGATAAATCAAAA
    TCTACAGACAGTGATAAATCAAAAGAGGATCAAGACAAAGCGACTAAAGATGAATCTGAT
    AATGATCAAAACAACGCTAATCAAGCGAACAATCAAGCACAAAATAATCAAAATCAACAA
    CAAGCTAATCAAAATCAACAACAGCAACAACAACGTCAAGGTGGTGGCCAAAGACATACA
    GTGAATGGTCAAGAAAACTTATACCGTATCGCAATTCAATACTACGGTTCAGGTTCACCG
    GAAAATGTTGAAAAAATTAGACGTGCCAATGGTTTAAGTGGTAACAATATTAGAAACGGT
    CAACAAATCGTTATTCCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1461

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1522 [new locus tag: SACOL_RS07760 ]
  • symbol: SACOL1522
  • description: elastin binding protein
  • length: 486
  • theoretical pI: 6.26146
  • theoretical MW: 53221
  • GRAVY: -1.557

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Elastin binding protein EbpS
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Elastin binding protein EbpS
  • PFAM:
    LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 25.1)
    and 2 more
    no clan defined DUF1510; Protein of unknown function (DUF1510) (PF07423; HMM-score: 19.5)
    Tetraspannin (CL0347) DUF4064; Protein of unknown function (DUF4064) (PF13273; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 8.28
    • Cellwall Score: 1.71
    • Extracellular Score: 0.01
    • Internal Helix: 1
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014107
    • TAT(Tat/SPI): 0.084419
    • LIPO(Sec/SPII): 0.003534
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENTEQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQEPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEAEQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNGKKAAAIGAGTAGVAGAAGAMAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLAGGAASKSASAASKPHASNNASQNHDEHDNHDRDKERKKGGMAKVLLPLIAAVLIIGALAIFGGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKEDQDKATKDESDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRIAIQYYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cell wall associated [1] [2] [3] [4] [5]
  • quantitative data / protein copy number per cell: 495 [6]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 1.24 h [7]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Annette Dreisbach, Kristina Hempel, Girbe Buist, Michael Hecker, Dörte Becher, Jan Maarten van Dijl
    Profiling the surfacome of Staphylococcus aureus.
    Proteomics: 2010, 10(17);3082-96
    [PubMed:20662103] [WorldCat.org] [DOI] (I p)
  4. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  5. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  6. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  7. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

T J Foster, M Höök
Surface protein adhesins of Staphylococcus aureus.
Trends Microbiol: 1998, 6(12);484-8
[PubMed:10036727] [WorldCat.org] [DOI] (P p)

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