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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1313 [new locus tag: SA_RS07445 ]
  • pan locus tag?: SAUPAN003947000
  • symbol: SA1313
  • pan gene symbol?: recQ2
  • synonym:
  • product: ATP-dependent DNA helicase RecQ

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1313 [new locus tag: SA_RS07445 ]
  • symbol: SA1313
  • product: ATP-dependent DNA helicase RecQ
  • replicon: chromosome
  • strand: -
  • coordinates: 1520407..1521786
  • length: 1380
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTTGCATGATATTTTACGAAACAAATTTGGCTTCGAGAATTTTAAACCGGGACAACAG
    GAAATTATAGAAAGTATAATGTCTCAACAACACACTCTAGGTATTCTTCCAACTGGAAGT
    GGAAAGAGTTTGTGTTATCAAATACCTACGTATTTATCAGGTAAGCCGACATTAATTATC
    TCACCGTTAATATCTTTAATGGATGACCAAGTTATGCAGTTGAAAATAAATGGAGAAAAA
    CGTGTAACATGTATTCACTCTGGTATGGATGAAATTGAGAAAAAGCATAATATTAAATGT
    TTACTACATAGCCGCTTCATCTTTCTAAGTCCAGAATTTCTCCTGCAACCGTCAAATTTT
    AAATTAATATCTATGATAGACTTTGGCATGATTGTTCTAGATGAAGCACATTGCCTATCT
    GAATGGGGATATGATTTCAGACCACATTATGCTCTAATAGGAAAAGTAACAAAGCATTTT
    AAAGAAGCGGTTGTCTTAGCGTTGACAGCAACTGCACCACCGCATTTACAAGATGATTTG
    ACGGAAATGTTAGCGATTCAATTCAACGTTATTAAAACTACAATGAATCGCCCAAACATA
    AGCTTTAAGCATCTTAATTTTCATGATGATGAAGATAAAATTGAGTGGTTGCTGCCGTTT
    CTACAACAGTCGGGACCAACGATTATCTATGTCTCATCGAAAAAGATGTGTCTGAATTTA
    GCGCAACTTATTTATGATTCAGGTTTTCTTACTGGCATTTATCATGGTGATATGAATTAT
    CAAGAGCGACACACAGTTCAACAACAATTTTTAAATAATGATATTCCGATTATAGTCGCA
    ACGAGTGCTTTTGGTATGGGTATTAATAAAAAAGATATTCGCACAATCATTCACTTTCAT
    CTTTCAACAAGTCCTTCTAACTACATTCAAGAAATTGGCCGTGCAGGTCGCGATGGTGAA
    CTAAGTCAGGCAATTAGTTTATTTCAACCGGACGATAAATATATTTTAGAAACGTTATTA
    TTTGCAGATATGATAACAGAAGAAGATGTACAAAATTTCGAAATAGGAGAATTTTTAGCA
    CCCGATAAACAAGACGTTTTGACAACGTTGCATTCATTCTATAGTATCGGCGCCTTGAAA
    CAGATATTTAAGCAATCATTTAAACGAAAGCAATTAGGATTCTTTCGCATGATTGGCTAT
    TGCAAATTGGATCAATGTAGACGGAAGTATTTATTAGAATTTTTCGGTGAATATCCACCG
    GCACAAGATCGATGTTGTGACAATGATTCTAATATAACTGATATCGTAATTTTAAATAAG
    AAGAAGGTAATTAGAAGTATTGGATTTGATGAAAAGTTGCAAAATTTATTTCTCAGATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1313 [new locus tag: SA_RS07445 ]
  • symbol: SA1313
  • description: ATP-dependent DNA helicase RecQ
  • length: 459
  • theoretical pI: 6.82561
  • theoretical MW: 52795.8
  • GRAVY: -0.157081

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 345.1)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 338.1)
    and 9 more
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 43.7)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 28.5)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair reverse gyrase (TIGR01054; EC 3.6.4.12,5.99.1.3; HMM-score: 21.4)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 16.9)
    DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 16.6)
    CRISPR-associated helicase Cas3, subtype CYANO (TIGR03158; HMM-score: 13.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 13.3)
    glutaredoxin-like protein NrdH (TIGR02194; HMM-score: 12.6)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair putative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 12.1)
  • TheSEED  :
    • ATP-dependent DNA helicase RecS (RecQ family)
  • PFAM:
    P-loop_NTPase (CL0023) Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 82.3)
    DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 71)
    and 2 more
    no clan defined RecQ_Zn_bind; RecQ zinc-binding (PF16124; HMM-score: 31.6)
    P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 31.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015277
    • TAT(Tat/SPI): 0.000755
    • LIPO(Sec/SPII): 0.000709
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLHDILRNKFGFENFKPGQQEIIESIMSQQHTLGILPTGSGKSLCYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLLHSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHFKEAVVLALTATAPPHLQDDLTEMLAIQFNVIKTTMNRPNISFKHLNFHDDEDKIEWLLPFLQQSGPTIIYVSSKKMCLNLAQLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIPIIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKYILETLLFADMITEEDVQNFEIGEFLAPDKQDVLTTLHSFYSIGALKQIFKQSFKRKQLGFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIVILNKKKVIRSIGFDEKLQNLFLR

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SA0505(fus)elongation factor G  [1] (data from MRSA252)
    SA0945(pdhC)branched-chain alpha-keto acid dehydrogenase E2 subunit  [1] (data from MRSA252)
    SA1099(rpsB)30S ribosomal protein S2  [1] (data from MRSA252)
    SAS052(rpsD)30S ribosomal protein S4  [1] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [1] (data from MRSA252)
    SA1314hypothetical protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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