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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1348 [new locus tag: SA_RS07640 ]
  • pan locus tag?: SAUPAN004072000
  • symbol: bfmBAA
  • pan gene symbol?: bfmBAA
  • synonym: bkdAA
  • product: branched-chain alpha-keto acid dehydrogenase E1

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1348 [new locus tag: SA_RS07640 ]
  • symbol: bfmBAA
  • product: branched-chain alpha-keto acid dehydrogenase E1
  • replicon: chromosome
  • strand: -
  • coordinates: 1557790..1558782
  • length: 993
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTGATTATAAATCATTAGGCCTTAGCGAAGAAGACCTAAAAGTAATATATAAATGG
    ATGGATTTAGGTAGAAAAATAGATGAAAGACTGTGGTTATTGAACCGTGCAGGTAAAATT
    CCATTTGTTGTAAGTGGTCAAGGACAAGAAGCAACTCAGATTGGGATGGCATACGCATTA
    GAAGAAGGTGATATTACAGCGCCTTATTATAGAGATCTAGCTTTCGTTACTTATATGGGT
    ATCTCAGCGTATGATACGTTTTTATCAGCGTTTGGAAAGAAAGACGATGTTAACTCTGGT
    GGAAAACAAATGCCATCACACTTTAGTAGTAGAGCTAAGAATATTTTGTCTCAAAGTTCA
    CCGGTAGCAACTCAAATACCCCATGCTGTTGGTGCTGCTTTAGCTTTAAAAATGGATGGT
    AAGAAGAAAATTGCAACAGCAACTGTTGGTGAAGGTAGCTCAAACCAAGGTGACTTCCAT
    GAAGGTTTAAACTTCGCGGGTGTACACAAACTTCCTTTTGTATGTGTCATTATAAATAAT
    AAATATGCAATTTCAGTACCTGATTCATTACAATACGCAGCTGAAAAATTATCGGATAGA
    GCACTTGGTTACGGTATACATGGTGAACAAGTAGATGGTAATGATCCATTAGCAATGTAT
    AAAGCGATGAAAGAAGCAAGAGATCGAGCTATTTCTGGTCAAGGTTCAACTTTGATTGAA
    GCAGTAACAAGTCGTATGACAGCTCATTCATCAGATGATGACGACCAATATCGTACGAAA
    GAAGAGCGTGAAGCGCTTAAAAAAGCTGACTGCAATGAAAAGTTCAAAAAAGAGTTGCTT
    TCAGCTGGCATTATCGATGATGCTTGGTTGGCAGAAATAGAAGCGGAGCATAAAGACATC
    ATCAATAAAGCGACAAAGGCTGCTGAAGACGCGCCATATCCAAGTGTCGAAGAAGCCTAT
    GCATTTGTGTACGAAGAAGGGAGTCTTAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    993

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1348 [new locus tag: SA_RS07640 ]
  • symbol: BfmBAA
  • description: branched-chain alpha-keto acid dehydrogenase E1
  • length: 330
  • theoretical pI: 4.94909
  • theoretical MW: 36199.5
  • GRAVY: -0.391515

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 316.9)
    and 5 more
    Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 223.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.3)
    Metabolism Energy metabolism TCA cycle oxoglutarate dehydrogenase (succinyl-transferring), E1 component (TIGR00239; EC 1.2.4.2; HMM-score: 11.3)
  • TheSEED  :
    • Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)
    Amino Acids and Derivatives Branched-chain amino acids Isoleucine degradation  Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)
    and 2 more
    Amino Acids and Derivatives Branched-chain amino acids Valine degradation  Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)
    Carbohydrates Central carbohydrate metabolism Dehydrogenase complexes  Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)
  • PFAM:
    THDP-binding (CL0254) E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 282.8)
    and 4 more
    TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 24)
    DXP_synthase_N; 1-deoxy-D-xylulose-5-phosphate synthase (PF13292; HMM-score: 21.7)
    XFP_N; XFP N-terminal domain (PF09364; HMM-score: 13.9)
    B_GA (CL0598) GA-like; GA-like domain (PF17573; HMM-score: 8.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007595
    • TAT(Tat/SPI): 0.003963
    • LIPO(Sec/SPII): 0.000405
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVVSGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGKQMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFHEGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLAMYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEEREALKKADCNEKFKKELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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