PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1466 [new locus tag: SAUSA300_RS08005 ]
• pan locus tag^?: SAUPAN004072000
• symbol: SAUSA300_1466
• pan gene symbol^?: bfmBAA
• synonym: bkdAA
• product: 2-oxoisovalerate dehydrogenase, E1 component, alpha subunit

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1466 [new locus tag: SAUSA300_RS08005 ]
• symbol: SAUSA300_1466
• product: 2-oxoisovalerate dehydrogenase, E1 component, alpha subunit
• replicon: chromosome
• strand: -
• coordinates: 1620347..1621339
• length: 993
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913598 NCBI
• RefSeq: YP_494162 NCBI
• BioCyc: see SAUSA300_RS08005
• MicrobesOnline: 1292981 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGATTGATTATAAATCATTAGGCCTTAGCGAAGAAGACCTAAAAGTAATATATAAATGG
  ATGGATTTAGGTAGAAAAATAGATGAAAGACTGTGGTTATTGAACCGTGCAGGTAAAATT
  CCATTTGTTGTAAGTGGTCAAGGACAAGAAGCAACTCAGATTGGGATGGCATACGCATTA
  GAAGAAGGTGATATTACAGCGCCTTATTATAGAGATCTAGCTTTCGTTACTTATATGGGT
  ATCTCAGCGTATGATACGTTTTTATCAGCGTTTGGAAAGAAAGACGATGTTAACTCTGGT
  GGAAAACAAATGCCATCACACTTTAGTAGTAGAGCTAAGAATATTTTGTCTCAAAGTTCA
  CCGGTAGCAACTCAAATACCCCATGCTGTTGGTGCTGCTTTAGCTTTAAAAATGGATGGT
  AAGAAGAAAATTGCAACAGCAACTGTTGGTGAAGGTAGCTCAAACCAAGGTGACTTCCAT
  GAAGGTTTAAACTTCGCGGGTGTACACAAACTTCCTTTTGTATGTGTCATTATAAATAAT
  AAATATGCAATTTCAGTACCTGATTCATTACAATACGCAGCTGAAAAATTATCGGATAGA
  GCACTTGGTTACGGTATACATGGTGAACAAGTAGATGGTAATGATCCATTAGCAATGTAT
  AAAGCGATGAAAGAAGCAAGAGATCGAGCTATTTCTGGTCAAGGTTCAACTTTGATTGAA
  GCAGTAACAAGTCGTATGACAGCTCATTCATCAGATGATGACGACCAATATCGTACGAAA
  GAAGAGCGTGAAACGCTTAAAAAAGCAGACTGCAATGAAAAGTTCAAAAAAGAGTTGCTT
  TCAGCTGGCATTATCGATGATGCTTGGTTGGCAGAAATAGAAGCGGAGCATAAAGACATC
  ATCAATAAAGCGACAAAGGCTGCTGAAGACGCGCCATATCCAAGTGTCGAAGAAGCCTAT
  GCATTTGTGTACGAAGAAGGGAGTCTTAACTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1466 [new locus tag: SAUSA300_RS08005 ]
• symbol: SAUSA300_1466
• description: 2-oxoisovalerate dehydrogenase, E1 component, alpha subunit
• length: 330
• theoretical pI: 4.94909
• theoretical MW: 36229.5
• GRAVY: -0.399091

⊟Function

• reaction:
  EC 1.2.4.1?  ExPASy
  Pyruvate dehydrogenase (acetyl-transferring) Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO₂
• TIGRFAM:
  Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 316.8)
  and 5 more
  Metabolism Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 223.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.4)
  Metabolism Energy metabolism TCA cycle oxoglutarate dehydrogenase (succinyl-transferring), E1 component (TIGR00239; EC 1.2.4.2; HMM-score: 10.9)
• TheSEED  :
  • Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)
  Amino Acids and Derivatives Branched-chain amino acids Isoleucine degradation  Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)
  and 2 more
  Amino Acids and Derivatives Branched-chain amino acids Valine degradation  Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)
  Carbohydrates Central carbohydrate metabolism Dehydrogenase complexes  Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4)
• PFAM:
  THDP-binding (CL0254) E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 282.4)
  and 3 more
  TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 24)
  DXP_synthase_N; 1-deoxy-D-xylulose-5-phosphate synthase (PF13292; HMM-score: 21.7)
  XFP_N; XFP N-terminal domain (PF09364; HMM-score: 13.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007595
  • TAT(Tat/SPI): 0.003963
  • LIPO(Sec/SPII): 0.000405
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162134 NCBI
• RefSeq: YP_494162 NCBI
• UniProt: A0A0H2XK21 UniProt

⊟Protein sequence

• MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVVSGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGKQMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFHEGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLAMYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFKKELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1464 < SAUSA300_1465 < SAUSA300_1466 < lpdA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications