Jump to navigation
Jump to search
NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS08005 [old locus tag: SAUSA300_1466 ]
- pan locus tag?: SAUPAN004072000
- symbol: SAUSA300_RS08005
- pan gene symbol?: bfmBAA
- synonym:
- product: 2-oxoisovalerate dehydrogenase subunit alpha
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS08005 [old locus tag: SAUSA300_1466 ]
- symbol: SAUSA300_RS08005
- product: 2-oxoisovalerate dehydrogenase subunit alpha
- replicon: chromosome
- strand: -
- coordinates: 1620347..1621339
- length: 993
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (1620347..1621339) NCBI
- BioCyc: SAUSA300_RS08005 BioCyc
- MicrobesOnline: see SAUSA300_1466
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961ATGATTGATTATAAATCATTAGGCCTTAGCGAAGAAGACCTAAAAGTAATATATAAATGG
ATGGATTTAGGTAGAAAAATAGATGAAAGACTGTGGTTATTGAACCGTGCAGGTAAAATT
CCATTTGTTGTAAGTGGTCAAGGACAAGAAGCAACTCAGATTGGGATGGCATACGCATTA
GAAGAAGGTGATATTACAGCGCCTTATTATAGAGATCTAGCTTTCGTTACTTATATGGGT
ATCTCAGCGTATGATACGTTTTTATCAGCGTTTGGAAAGAAAGACGATGTTAACTCTGGT
GGAAAACAAATGCCATCACACTTTAGTAGTAGAGCTAAGAATATTTTGTCTCAAAGTTCA
CCGGTAGCAACTCAAATACCCCATGCTGTTGGTGCTGCTTTAGCTTTAAAAATGGATGGT
AAGAAGAAAATTGCAACAGCAACTGTTGGTGAAGGTAGCTCAAACCAAGGTGACTTCCAT
GAAGGTTTAAACTTCGCGGGTGTACACAAACTTCCTTTTGTATGTGTCATTATAAATAAT
AAATATGCAATTTCAGTACCTGATTCATTACAATACGCAGCTGAAAAATTATCGGATAGA
GCACTTGGTTACGGTATACATGGTGAACAAGTAGATGGTAATGATCCATTAGCAATGTAT
AAAGCGATGAAAGAAGCAAGAGATCGAGCTATTTCTGGTCAAGGTTCAACTTTGATTGAA
GCAGTAACAAGTCGTATGACAGCTCATTCATCAGATGATGACGACCAATATCGTACGAAA
GAAGAGCGTGAAACGCTTAAAAAAGCAGACTGCAATGAAAAGTTCAAAAAAGAGTTGCTT
TCAGCTGGCATTATCGATGATGCTTGGTTGGCAGAAATAGAAGCGGAGCATAAAGACATC
ATCAATAAAGCGACAAAGGCTGCTGAAGACGCGCCATATCCAAGTGTCGAAGAAGCCTAT
GCATTTGTGTACGAAGAAGGGAGTCTTAACTAA60
120
180
240
300
360
420
480
540
600
660
720
780
840
900
960
993
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS08005 [old locus tag: SAUSA300_1466 ]
- symbol: SAUSA300_RS08005
- description: 2-oxoisovalerate dehydrogenase subunit alpha
- length: 330
- theoretical pI: 4.94909
- theoretical MW: 36229.5
- GRAVY: -0.399091
⊟Function[edit | edit source]
- reaction: EC 1.2.4.1? ExPASyPyruvate dehydrogenase (acetyl-transferring) Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2
- TIGRFAM: Energy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 316.8)and 5 moreEnergy metabolism Pyruvate dehydrogenase pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 223.5)Biosynthesis of cofactors, prosthetic groups, and carriers Other 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.4)Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.4)Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine 1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 20.4)Energy metabolism TCA cycle oxoglutarate dehydrogenase (succinyl-transferring), E1 component (TIGR00239; EC 1.2.4.2; HMM-score: 10.9)
- TheSEED: see SAUSA300_1466
- PFAM: THDP-binding (CL0254) E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 282.4)and 3 moreTPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 24)DXP_synthase_N; 1-deoxy-D-xylulose-5-phosphate synthase (PF13292; HMM-score: 21.7)XFP_N; XFP N-terminal domain (PF09364; HMM-score: 13.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.67
- Cytoplasmic Membrane Score: 0.01
- Cellwall Score: 0.15
- Extracellular Score: 0.17
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.007595
- TAT(Tat/SPI): 0.003963
- LIPO(Sec/SPII): 0.000405
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446490499 NCBI
- RefSeq: WP_000568353 NCBI
- UniProt: see SAUSA300_1466
⊟Protein sequence[edit | edit source]
- MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVVSGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGKQMPSHFSSRAKNILSQSSPVATQIPHAVGAALALKMDGKKKIATATVGEGSSNQGDFHEGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLAMYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEERETLKKADCNEKFKKELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.