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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA1412 [new locus tag: SA_RS07970 ]
- pan locus tag?: SAUPAN004166000
- symbol: hemN
- pan gene symbol?: hemN
- synonym:
- product: coproporphyrinogen III oxidase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA1412 [new locus tag: SA_RS07970 ]
- symbol: hemN
- product: coproporphyrinogen III oxidase
- replicon: chromosome
- strand: -
- coordinates: 1617151..1618275
- length: 1125
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1124253 NCBI
- RefSeq: NP_374696 NCBI
- BioCyc: see SA_RS07970
- MicrobesOnline: 103722 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1081ATGACGGTACAAAGTGCATATATACATATTCCATTTTGTGTAAGAATATGTACATATTGT
GATTTCAATAAATATTTTATACAGAATCAACCTGTAGATGAGTACTTAGATGCACTAATA
ACAGAAATGTCTACAGCAAAATATAGGAACTTAAAGACCATGTATGTAGGTGGCGGCACA
CCAACGGCCCTTTCTATTAATCAGTTGGAAAGATTACTTAAAGCAATACGTGATACGTTT
ACAATCACAGGCGAGTATACATTTGAAGCAAATCCTGATGAGTTAACTAAAGAGAAAGTC
CAACTATTAGAGAAATATGGAGTAAATAGAATTTCAATGGGCGTTCAAACATTCAAACCG
GAGTTATTGTCTGTTTTAGGTAGAACGCACAATACTGAAGATATTTACACTTCGGTGTTA
AATGCTAAAAACGCAGGTATTAAATCAATCAGTTTAGATTTAATGTATCATTTACCGAAA
CAGACGATTGAAGATTTTGAACAAAGTTTAGATCTAGCTTTAGATATGGATATTCAACAT
ATTTCGAGTTACGGCTTAATACTTGAACCTAAAACCCAATTTTATAATATGTATAGAAAA
GGGTTGCTTAAACTTCCTAATGAGGATTTAGGTGCTGACATGTATCAGTTGTTGATGTCT
AAGATAGAACAATCTCCTTTCCATCAATACGAAATATCTAATTTTGCATTAGATGGCCAT
GAATCAGAACATAATAAGGTTTACTGGTTTAATGAGGAATATTATGGATTTGGAGCAGGT
GCAAGTGGTTATGTAGATGGTGTGCGTTATACGAATATCAATCCAGTGAATCATTATATC
AAAGCTATAAATAAAGAAAGTAAAGCAATTTTAGTATCAAATAAACCTTCTTTGACTGAG
AGAATGGAAGAAGAAATGTTTCTTGGGTTGCGTTTAAATGAAGGTGTGAGTAGTAGTAGG
TTCAAAAAGAAGTTTGACCAATCTATTGAAAGTGTCTTTGGTCAAACAATAAATAATTTA
AAAGAGAAAGAATTAATTGTAGAAAAGAACGATGCGATTGCACTAACAAAGCGAGGGAAA
GTCATAGGTAATGAGGTTTTTGAAGCTTTCCTAATAAATGATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA1412 [new locus tag: SA_RS07970 ]
- symbol: HemN
- description: coproporphyrinogen III oxidase
- length: 374
- theoretical pI: 5.31963
- theoretical MW: 42839.5
- GRAVY: -0.351872
⊟Function[edit | edit source]
- reaction: EC 1.3.3.3? ExPASyCoproporphyrinogen oxidase Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2OEC 1.3.99.-? ExPASy
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin putative oxygen-independent coproporphyrinogen III oxidase (TIGR00539; EC 1.3.99.22; HMM-score: 379)and 21 moreputative heme utilization radical SAM enzyme HutW (TIGR04107; HMM-score: 210.8)Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin coproporphyrinogen dehydrogenase HemZ (TIGR03994; EC 1.3.99.22; HMM-score: 161.5)Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin oxygen-independent coproporphyrinogen III oxidase (TIGR00538; EC 1.3.99.22; HMM-score: 156.3)HemN-related non-iron pseudo-SAM protein PsgB (TIGR04419; HMM-score: 82.8)Unknown function Enzymes of unknown specificity B12-binding domain/radical SAM domain protein, MJ_1487 family (TIGR04013; HMM-score: 48.7)Unknown function Enzymes of unknown specificity radical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 46)Transcription DNA-dependent RNA polymerase radical SAM enzyme/protein acetyltransferase, ELP3 family (TIGR01211; EC 2.3.1.48; HMM-score: 39.8)Protein synthesis tRNA and rRNA base modification tRNA-i(6)A37 thiotransferase enzyme MiaB (TIGR01574; EC 2.-.-.-; HMM-score: 26.1)Unknown function Enzymes of unknown specificity B12-binding domain/radical SAM domain protein, MJ_0865 family (TIGR04014; HMM-score: 25)radical SAM methylthiotransferase, MiaB/RimO family (TIGR00089; EC 2.1.1.-,2.8.1.-; HMM-score: 21.5)Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdenum cofactor biosynthesis protein A (TIGR02666; HMM-score: 21.4)putative metalloenzyme radical SAM/SPASM domain maturase (TIGR04311; HMM-score: 19.1)Protein synthesis tRNA and rRNA base modification MiaB-like tRNA modifying enzyme (TIGR01579; HMM-score: 17.7)Cellular processes Toxin production and resistance ribosomal peptide maturation radical SAM protein 1 (TIGR03975; HMM-score: 17.5)Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis protein ThiH (TIGR02351; HMM-score: 17.2)Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone putative menaquinone biosynthesis radical SAM enzyme, SCO4494 family (TIGR03700; HMM-score: 15.6)Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin probable molybdenum cofactor biosynthesis protein A (TIGR02668; HMM-score: 14.4)hopanoid biosynthesis associated radical SAM protein HpnJ (TIGR03471; HMM-score: 13.7)Biosynthesis of cofactors, prosthetic groups, and carriers Other coenzyme PQQ biosynthesis enzyme PqqE (TIGR02109; HMM-score: 13.1)Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone dehypoxanthine futalosine cyclase (TIGR03699; HMM-score: 12.7)Biosynthesis of cofactors, prosthetic groups, and carriers Other 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofH subunit (TIGR03551; EC 2.5.1.77; HMM-score: 11.1)
- TheSEED :
- Hypothetical radical SAM family enzyme in heat shock gene cluster, similarity with CPO of BS HemN-type
Cofactors, Vitamins, Prosthetic Groups, Pigments Tetrapyrroles CPO analysis Hypothetical radical SAM family enzyme in heat shock gene cluster, similarity with CPO of BS HemN-typeand 1 more - PFAM: TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 75.2)and 2 moreHTH (CL0123) HemN_C; HemN C-terminal domain (PF06969; HMM-score: 55.3)4Fe-4S (CL0344) Fer4_14; 4Fe-4S single cluster domain (PF13394; HMM-score: 14.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: [4Fe-4S] cluster
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.019458
- TAT(Tat/SPI): 0.000182
- LIPO(Sec/SPII): 0.009979
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMSTAKYRNLKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQLLEKYGVNRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPKQTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLLMSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPVNHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFGQTINNLKEKELIVEKNDAIALTKRGKVIGNEVFEAFLIND
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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