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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1640 [new locus tag: SACOL_RS08360 ]
  • pan locus tag?: SAUPAN004166000
  • symbol: SACOL1640
  • pan gene symbol?: hemN
  • synonym:
  • product: coproporphyrinogen III oxidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1640 [new locus tag: SACOL_RS08360 ]
  • symbol: SACOL1640
  • product: coproporphyrinogen III oxidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1670210..1671367
  • length: 1158
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    TTGGATAAAAAGTCTGAGAAGCGGGGCATTAAAATGACGGTACAAAGTGCATATATACAT
    ATTCCATTTTGTGTAAGAATATGTACATATTGTGATTTCAATAAATATTTTATACAGAAT
    CAACCTGTAGATGAGTACTTAGATGCACTAATCACAGAAATGTCTACAGCAAAATATAGG
    ATCTTAAAGACCATGTATGTAGGTGGCGGCACACCAACGGCCCTTTCTATTAATCAGTTG
    GAAAGATTACTTAAAGCAATACGTGATACGTTTACAATCACAGGCGAGTATACATTTGAA
    GCAAATCCTGATGAGTTAACTAAAGAGAAAGTCCAACTATTAGAGAAATATGGAGTAAAA
    AGGATTTCAATGGGCGTTCAAACATTCAAGCCGGAGTTATTGTCTGTTTTAGGTAGAACG
    CACAATACTGAAGATATTTACACTTCGGTGTTAAATGCTAAAAACGCAGGTATTAAATCA
    ATCAGTTTAGATTTAATGTATCATTTACCGAAACAGACGATTGAAGATTTTGAACAAAGT
    TTAGATCTAGCTTTAGATATGGATATTCAACATATTTCGAGTTACGGCTTAATACTTGAA
    CCTAAAACCCAATTTTATAATATGTATAGAAAAGGCTTGCTCAAACTTCCTAATGAGGAT
    TTAGGTGCTGACATGTATCAGTTGCTGATGTCTAAGATAGAACAATCTCCTTTCCATCAA
    TACGAAATATCTAATTTTGCATTAGATGGCCATGAATCAGAACATAATAAGGTTTACTGG
    TTTAATGAGGAATATTATGGATTTGGAGCAGGTGCAAGTGGTTATGTAGATGGTGTGCGT
    TATACGAATATCAATCCAGTGAATCATTATATCAAAGCTATAAATAAAGAAAGTAAAGCA
    ATTTTAGTATCAAATAAACCTTCTTTGACTGAGAGAATGGAAGAAGAAATGTTTCTTGGG
    TTGCGTTTAAATGAAGGTGTGAGTAGTAGTAGGTTCAAAAAGAAGTTTGACCAATCTATT
    GAAAGTGTCTTTGGTCAAACAATAAATAATTTAAAAGAGAAGGAATTAATTGTAGAAAAG
    AACGATGTGATTGCACTTACAAATAGAGGGAAAGTCATAGGTAATGAGGTTTTTGAAGCT
    TTCCTAATAAATGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1158

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1640 [new locus tag: SACOL_RS08360 ]
  • symbol: SACOL1640
  • description: coproporphyrinogen III oxidase
  • length: 385
  • theoretical pI: 5.91968
  • theoretical MW: 44168.2
  • GRAVY: -0.371688

⊟Function[edit | edit source]

  • reaction:
    EC 1.3.99.-?  ExPASy
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin putative oxygen-independent coproporphyrinogen III oxidase (TIGR00539; EC 1.3.99.22; HMM-score: 375)
    and 21 more
    putative heme utilization radical SAM enzyme HutW (TIGR04107; HMM-score: 207.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin coproporphyrinogen dehydrogenase HemZ (TIGR03994; EC 1.3.99.22; HMM-score: 160.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin oxygen-independent coproporphyrinogen III oxidase (TIGR00538; EC 1.3.99.22; HMM-score: 150.6)
    HemN-related non-iron pseudo-SAM protein PsgB (TIGR04419; HMM-score: 83.6)
    Unknown function Enzymes of unknown specificity B12-binding domain/radical SAM domain protein, MJ_1487 family (TIGR04013; HMM-score: 48.1)
    Unknown function Enzymes of unknown specificity radical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 45.6)
    Genetic information processing Transcription DNA-dependent RNA polymerase radical SAM enzyme/protein acetyltransferase, ELP3 family (TIGR01211; EC 2.3.1.48; HMM-score: 39.5)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA-i(6)A37 thiotransferase enzyme MiaB (TIGR01574; EC 2.-.-.-; HMM-score: 25.8)
    Unknown function Enzymes of unknown specificity B12-binding domain/radical SAM domain protein, MJ_0865 family (TIGR04014; HMM-score: 23.5)
    radical SAM methylthiotransferase, MiaB/RimO family (TIGR00089; EC 2.1.1.-,2.8.1.-; HMM-score: 21.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdenum cofactor biosynthesis protein A (TIGR02666; HMM-score: 21.3)
    putative metalloenzyme radical SAM/SPASM domain maturase (TIGR04311; HMM-score: 17.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification MiaB-like tRNA modifying enzyme (TIGR01579; HMM-score: 17.5)
    Cellular processes Cellular processes Toxin production and resistance ribosomal peptide maturation radical SAM protein 1 (TIGR03975; HMM-score: 17.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis protein ThiH (TIGR02351; HMM-score: 14.8)
    hopanoid biosynthesis associated radical SAM protein HpnJ (TIGR03471; HMM-score: 14.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin probable molybdenum cofactor biosynthesis protein A (TIGR02668; HMM-score: 14)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone putative menaquinone biosynthesis radical SAM enzyme, SCO4494 family (TIGR03700; HMM-score: 13.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other coenzyme PQQ biosynthesis enzyme PqqE (TIGR02109; HMM-score: 12.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone dehypoxanthine futalosine cyclase (TIGR03699; HMM-score: 11.6)
    pseudo-rSAM protein/SPASM domain protein (TIGR04347; HMM-score: 11.2)
  • TheSEED  :
    • Hypothetical radical SAM family enzyme in heat shock gene cluster, similarity with CPO of BS HemN-type
    Cofactors, Vitamins, Prosthetic Groups, Pigments Tetrapyrroles CPO analysis  Hypothetical radical SAM family enzyme in heat shock gene cluster, similarity with CPO of BS HemN-type
    and 1 more
    Stress Response Heat shock Heat shock dnaK gene cluster extended  Hypothetical radical SAM family enzyme in heat shock gene cluster, similarity with CPO of BS HemN-type
  • PFAM:
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 73)
    and 2 more
    HTH (CL0123) HemN_C; HemN C-terminal domain (PF06969; HMM-score: 52)
    4Fe-4S (CL0344) Fer4_14; 4Fe-4S single cluster domain (PF13394; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: [4Fe-4S] cluster
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.046168
    • TAT(Tat/SPI): 0.000608
    • LIPO(Sec/SPII): 0.053607
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDKKSEKRGIKMTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMSTAKYRILKTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQLLEKYGVKRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPKQTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLLMSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPVNHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFKKKFDQSIESVFGQTINNLKEKELIVEKNDVIALTNRGKVIGNEVFEAFLIND

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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