PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1497 [new locus tag: SA_RS08425 ]
• pan locus tag^?: SAUPAN004288000
• symbol: engB
• pan gene symbol^?: engB
• synonym:
• product: ribosome biogenesis GTP-binding protein YsxC

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1497 [new locus tag: SA_RS08425 ]
• symbol: engB
• product: ribosome biogenesis GTP-binding protein YsxC
• replicon: chromosome
• strand: -
• coordinates: 1705397..1705987
• length: 591
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124342 NCBI
• RefSeq: NP_374785 NCBI
• BioCyc: see SA_RS08425
• MicrobesOnline: 103811 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  
  ATGAAAGTTAATCCTAATAATATTGAATTAATCATTAGTGCAGTAAAAGAAGAACAATAT
  CCAGAAACAGAATTGTCTGAAGTTGCACTGAGCGGTCGATCTAATGTAGGTAAGTCTACA
  TTTATCAATAGTATGATTGGCAGAAAAAATATGGCACGTACATCACAGCAACCCGGCAAA
  ACGCAAACGTTAAATTTTTATAATATAGATGAACAACTTATTTTTGTGGATGTTCCAGGG
  TATGGATATGCTAAAGTAAGTAAAACACAACGTGAAAAATTTGGGAAAATGATTGAGGAA
  TATATAACTAAGAGAGAGAATTTGCAATTAGTTATTCAATTAGTTGATTTAAGACATGAT
  CCAACACAAGATGATATCTTAATGTACAATTATTTGAAACATTTTGATATTCCTACTTTA
  GTTATATGCACTAAAGAAGACAAAATTCCAAAAGGTAAAGTTCAAAAGCATATTAAAAAT
  ATTAAGACACAATTAGATATGGACCCAGACGATACAATTGTAAGTTATTCATCAATTCAA
  AATAATAAACAACAACAAATATGGAATTTAATTGAACCGTATATTTCATAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  591
  

⊟Protein

⊟General

• locus tag: SA1497 [new locus tag: SA_RS08425 ]
• symbol: EngB
• description: ribosome biogenesis GTP-binding protein YsxC
• length: 196
• theoretical pI: 7.58416
• theoretical MW: 22684.8
• GRAVY: -0.585204

⊟Function

• TIGRFAM:
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 225.8)
  and 13 more
  Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 63.9)
  Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 60.6)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 50.3)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 41.3)
  Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 37)
  Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 35.1)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 33.1)
  Genetic information processing Protein synthesis Other Obg family GTPase CgtA (TIGR02729; HMM-score: 28)
  Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 23.8)
  Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 19.7)
  Genetic information processing Protein synthesis Translation factors ribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 15.5)
  Metabolism Transport and binding proteins Amino acids, peptides and amines chloroplast protein import component Toc86/159, G and M domains (TIGR00993; HMM-score: 14)
  Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtE (TIGR03410; HMM-score: 13.9)
• TheSEED  :
  • GTP-binding protein EngB
  Protein Metabolism Protein biosynthesis Universal GTPases  GTP-binding protein EngB
• PFAM:
  P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 74.9)
  and 12 more
  FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 37.6)
  Dynamin_N; Dynamin family (PF00350; HMM-score: 30.7)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 26.9)
  AIG1; AIG1 family (PF04548; HMM-score: 20.9)
  GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 16.5)
  Septin; Septin (PF00735; HMM-score: 15.2)
  IIGP; Interferon-inducible GTPase (IIGP) (PF05049; HMM-score: 15.2)
  cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 14.9)
  RNA_helicase; RNA helicase (PF00910; HMM-score: 13.8)
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 13.8)
  ABC_tran; ABC transporter (PF00005; HMM-score: 13.3)
  Cyclin (CL0065) RB_B; Retinoblastoma-associated protein B domain (PF01857; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.020891
  • TAT(Tat/SPI): 0.005097
  • LIPO(Sec/SPII): 0.002423
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927252 NCBI
• RefSeq: NP_374785 NCBI
• UniProt: P64071 UniProt

⊟Protein sequence

• MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINSMIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYITKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKNIKTQLDMDPDDTIVSYSSIQNNKQQQIWNLIEPYIS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications