PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1512 [new locus tag: SA_RS08515 ]
• pan locus tag^?: SAUPAN004312000
• symbol: SA1512
• pan gene symbol^?: mutM
• synonym:
• product: formamidopyrimidine-DNA glycosylase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1512 [new locus tag: SA_RS08515 ]
• symbol: SA1512
• product: formamidopyrimidine-DNA glycosylase
• replicon: chromosome
• strand: -
• coordinates: 1721209..1722081
• length: 873
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124357 NCBI
• RefSeq: NP_374800 NCBI
• BioCyc: see SA_RS08515
• MicrobesOnline: 103826 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGCCCGAATTACCAGAAGTAGAACATGTAAAAAGAGGAATTGAACCCTACGTTATAAAT
  CAAAAAATTGAACACGTTATATTTTCGGATAAAGTAATCGAAGGTAAAGCTCAAGGCAAG
  GAAACAATTATAAAAGGAATTGAATTAGATACTTTTAAAACTTTATCGGAAGGCTATACC
  ATTACAAATGTAGAACGAAGAAGTAAATATATCGTTTTTCAGCTAGATAATAAACGAGAA
  CAACGTACATTAATTAGTCATTTAGGTATGGCGGGTGGTTTTTTCATTGTAGATGAACTT
  GAAGATATTATGATACCTAATTATCGTAAGCATTGGCATGTTATTTTTGAGTTGTCAAAT
  GACAAAAAGTTAATTTATTCAGATATACGTCGTTTTGGAGAAATTAGAAATGTTGCGTCT
  GTAGCATCTTATCCTTCATTTTTAGAAATAGCGCCTGAACCTTTTACAAATGAAGCATTG
  ACGTATTATTTAAATCGAATTCATCAACAGTCTAATAAGAATAAACCAATCAAACAAGTG
  ATTTTGGATCATAAAGTAATCGCAGGTTGCGGAAATATTTACGCATGTGAAGCGTTGTTT
  CGTGCAGGTGTTTTACCAGATAAAAAAGTAAAAGACTTAACACACCAACAACAAGAAATG
  GTTTTTTATTATGTTAGAGAAGTACTAGAAGAAGGTATTAAGCATGGCGGTACAAGTATT
  TCAGATTATCGTCATGCTGATGGAAAAACTGGTGAGATGCAATTGCATCTTAATGTATAT
  AAGCAGCCGGTATGCAAAGTGTGTGGAAGTCAAATTGAAACAAAAATCATCGCGACAAGA
  AATAGTCATTATTGTCCAGTATGTCAAAAATAA
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⊟Protein

⊟General

• locus tag: SA1512 [new locus tag: SA_RS08515 ]
• symbol: SA1512
• description: formamidopyrimidine-DNA glycosylase
• length: 290
• theoretical pI: 8.1364
• theoretical MW: 33399.2
• GRAVY: -0.396897

⊟Function

• reaction:
  EC 3.2.2.23?  ExPASy
  DNA-formamidopyrimidine glycosylase Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine
  EC 4.2.99.18?  ExPASy
  DNA-(apurinic or apyrimidinic site) lyase The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA-formamidopyrimidine glycosylase (TIGR00577; EC 3.2.2.23; HMM-score: 234.4)
  and 1 more
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein uS13 (TIGR03631; HMM-score: 15.2)
• TheSEED  :
  • Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23) (EC 4.2.99.18)
  DNA Metabolism DNA repair DNA Repair Base Excision  Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23)
• PFAM:
  no clan defined Fapy_DNA_glyco; Formamidopyrimidine-DNA glycosylase N-terminal domain (PF01149; HMM-score: 99.2)
  and 3 more
  H2TH (CL0303) H2TH; Formamidopyrimidine-DNA glycosylase H2TH domain (PF06831; HMM-score: 69)
  Zn_Beta_Ribbon (CL0167) zf-FPG_IleRS; Zinc finger found in FPG and IleRS (PF06827; HMM-score: 24.8)
  H2TH (CL0303) FbpA; Fibronectin-binding protein A N-terminus (FbpA) (PF05833; HMM-score: 17.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005618
  • TAT(Tat/SPI): 0.000165
  • LIPO(Sec/SPII): 0.000521
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927267 NCBI
• RefSeq: NP_374800 NCBI
• UniProt: A0A0H3JMB1 UniProt

⊟Protein sequence

• MPELPEVEHVKRGIEPYVINQKIEHVIFSDKVIEGKAQGKETIIKGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELEDIMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFTNEALTYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQEMVFYYVREVLEEGIKHGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKIIATRNSHYCPVCQK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: coaE < SA1512 < polA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

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⊟Literature

⊟References

⊟Relevant publications