PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1546 [new locus tag: SA_RS08715 ]
• pan locus tag^?: SAUPAN004384000
• symbol: SA1546
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1546 [new locus tag: SA_RS08715 ]
• symbol: SA1546
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1767540..1768670
• length: 1131
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124392 NCBI
• RefSeq: NP_374835 NCBI
• BioCyc: see SA_RS08715
• MicrobesOnline: 103861 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  
  GTGAAGAAAATTTTATTTGATGTTGATGGCATTTTTTTAAGTGAAGAACGTTGTTTCGAT
  GTCTCTGCATTAACGGTATATGAATTATTAATGGATAAGTGTTATTTAGGTCTTCATTCA
  CATATAGATTGGGAAACTTTGACTGATAACGATATACAAGACATTAGGAATCGAATTTTT
  CAAAAAGATAAGATTTTAAACAAATTAAAATCGCTAGGGCTGAATTCAAATTGGGATATG
  TTATTTATCGTTTTTAGTATTCATTTAATTGATATTTTGAAAAAGTTATCACATGATGAA
  ATTGAGGCATTCATGTATCAAGATGAGCCTGTCGAATTGAAGTTGCAAAATATAAGCACA
  AACTTAGCAGACTGTTTTAATTTAAATGAACAACTACCTTTACAATTTTTGGATAATGTA
  AAAGTTGGTAAAAATAATATTTATGCTGCTTTGGAAGAGTTTGCAACAACAGAGTTACAT
  GTTTCGGATGCTACTTTATTTAGTTTGAAGGGTGCATTATGGACGTTAGCGCAAGAAGTT
  TATCAAGAATGGTATTTAGGATCGAAGTTGTATGAAGATGTTGAAAAGAAAATAGCACGA
  ACTACTTTTAAGACAGGTTATATTTATCAAGAAATTATTTTGAGACCAGTAGATGAAGTT
  AAGGTACTTCTGAATGATTTAAAAGGTGCTGGTTTCGAATTAGGTATTGCAACAGGTCGT
  CCTTATACTGAGACTGTTGTGCCATTTGAAAATTTAGGATTGTTACCATATTTTGAAGCT
  GATTTTATTGCAACAGCAAGTGATGTTTTAGAAGCAGAGAATATGTATCCGCAAGCACGA
  CCATTAGGAAAGCCGAATCCTTTTAGTTATATCGCAGCTTTATATGGTAATAATCGCGAT
  AAATATGAATCTTATATCAATAAGCAAGATAACATTGTAAATAAAGATGACGTATTTATA
  GTAGGCGATTCGTTAGCTGACTTATTAAGTGCTCAAAAAATAGGTGCAACGTTTATTGGA
  ACATTAACAGGTTTAAAAGGTAAGGATGCTGCAGGTGAGTTAGAAGCGCATCATGCCGAC
  TATGTTATTAATCATTTAGGTGAACTTAGAGGTGTACTAGATAATTTGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1131
  

⊟Protein

⊟General

• locus tag: SA1546 [new locus tag: SA_RS08715 ]
• symbol: SA1546
• description: hypothetical protein
• length: 376
• theoretical pI: 4.46136
• theoretical MW: 42672.3
• GRAVY: -0.118617

⊟Function

• TIGRFAM:
  AHBA synthesis associated protein (TIGR01454; HMM-score: 30.6)
  HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 29.9)
  Metabolism Energy metabolism Sugars phosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 24.8)
  phosphonatase-like hydrolase (TIGR03351; HMM-score: 24.7)
  and 2 more
  HAD hydrolase, TIGR01548 family (TIGR01548; HMM-score: 16.6)
  beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 15.7)
• TheSEED  :
  • Phosphoserine phosphatase (EC 3.1.3.3)
  Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  Phosphoserine phosphatase (EC 3.1.3.3)
  and 1 more
  Amino Acids and Derivatives Alanine, serine, and glycine Serine Biosynthesis  Phosphoserine phosphatase (EC 3.1.3.3)
• PFAM:
  HAD (CL0137) Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 58.1)
  and 3 more
  HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 46.4)
  Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 20.1)
  Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003145
  • TAT(Tat/SPI): 0.000093
  • LIPO(Sec/SPII): 0.00059
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927302 NCBI
• RefSeq: NP_374835 NCBI
• UniProt: A0A0H3JMD6 UniProt

⊟Protein sequence

• MKKILFDVDGIFLSEERCFDVSALTVYELLMDKCYLGLHSHIDWETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIEAFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELHVSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVDEVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMYPQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKIGATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications