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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA1681 [new locus tag: SA_RS09645 ]
- pan locus tag?: SAUPAN004844000
- symbol: gsaB
- pan gene symbol?: gsaB
- synonym:
- product: glutamate-1-semialdehyde aminotransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA1681 [new locus tag: SA_RS09645 ]
- symbol: gsaB
- product: glutamate-1-semialdehyde aminotransferase
- replicon: chromosome
- strand: +
- coordinates: 1926659..1927948
- length: 1290
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1124564 NCBI
- RefSeq: NP_374971 NCBI
- BioCyc: see SA_RS09645
- MicrobesOnline: 103997 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1261ATGAATTTTAGTGAAAGTGAACGTTTACAACAACTTTCAAACGAATATATTCTAGGCGGT
GTCAATTCCCCTTCTCGTTCTTATAAAGCTGTAGGAGGCGGTGCACCTGTTGTTATGAAA
GAAGGACACGGTGCATATTTATATGATGTCGATGGCAATAAATTTATTGATTACCTTCAA
GCATACGGTCCAATTATTGCGGGGCATGCACATCCTCATATTACTAAAGCAATTCAAGAA
CAAGCAGCCAAAGGTGTTTTATTTGGTACACCGACTGAACTAGAAATTGAATTCAGCAAA
AAATTACGTGATGCAATTCCATCTCTTGAGAAAATTCGCTTTGTAAATTCTGGAACAGAA
GCAGTCATGACAACAATTCGTGTTGCACGTGCATATACTAAAAGAAATAAAATTATAAAA
TTTGCTGGATCTTATCATGGCCATTCTGATTTAGTATTGGTTGCAGCAGGTAGTGGCCCA
TCTCAGCTCGGTTCTCCAGACTCAGCTGGTGTTCCAGAAAGCGTCGCACGTGAAGTCATT
ACTGTACCTTTCAATGATATTAACGCATATAAAGAAGCAATTGAATTTTGGGGTGATGAA
ATTGCCGCAGTATTAGTAGAACCAATTGTTGGTAACTTTGGAATGGTAATGCCTCAACCT
GGATTTTTAGAAGAGGTTAATGAAATTTCGCATAACAATGGGACACTAGTGATTTATGAT
GAAGTAATTACTGCATTCCGTTTCCATTACGGTGCCGCTCAAGATTTATTAGGTGTTATC
CCTGATTTAACTGCATTTGGTAAAATTGTTGGCGGTGGTTTACCAATTGGAGGCTATGGT
GGACGTCAAGATATTATGGAACAAGTAGCACCTCTAGGACCTGCATATCAAGCTGGTACA
ATGGCTGGTAACCCGTTATCTATGAAAGCAGGTATTGCATTACTCGAAGTACTAGAACAA
GACGGTGTTTATGAAAAATTAGACAGCTTAGGCCAACAACTAGAAGAAGGTTTACTTAAA
TTAATCGAAAAACATAATATCACAGCTACAATTAATCGTATTTATGGATCTTTAACATTG
TACTTTACAGATGAAAAAGTCACACATTATGATCAAGTTGAACATTCTGACGGCGAAGCG
TTCGGTAAATTTTTCAAATTAATGTTAAATCAAGGTATCAATTTAGCACCTTCTAAGTTT
GAAGCTTGGTTCTTAACAACTGAACATACAGAAGAAGATATTCAACAAACTTTAAAAGCT
GCAGACTATGCTTTTAGTCAAATGAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA1681 [new locus tag: SA_RS09645 ]
- symbol: GsaB
- description: glutamate-1-semialdehyde aminotransferase
- length: 429
- theoretical pI: 4.83919
- theoretical MW: 46725.6
- GRAVY: -0.11352
⊟Function[edit | edit source]
- reaction: EC 5.4.3.8? ExPASyGlutamate-1-semialdehyde 2,1-aminomutase (S)-4-amino-5-oxopentanoate = 5-aminolevulinate
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamate-1-semialdehyde-2,1-aminomutase (TIGR00713; EC 5.4.3.8; HMM-score: 548)and 10 moretransaminase, acetylornithine/succinylornithine family (TIGR00707; HMM-score: 175)Central intermediary metabolism Other 4-aminobutyrate transaminase (TIGR00700; EC 2.6.1.19; HMM-score: 157.9)ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 155.6)Energy metabolism Amino acids and amines succinylornithine transaminase family (TIGR03246; EC 2.6.1.81; HMM-score: 133.1)Biosynthesis of cofactors, prosthetic groups, and carriers Biotin adenosylmethionine-8-amino-7-oxononanoate transaminase (TIGR00508; EC 2.6.1.62; HMM-score: 129.2)Central intermediary metabolism Polyamine biosynthesis putrescine aminotransferase (TIGR03372; EC 2.6.1.82; HMM-score: 111.7)Central intermediary metabolism Other 2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 108.1)Cellular processes Adaptations to atypical conditions diaminobutyrate--2-oxoglutarate aminotransferase (TIGR02407; EC 2.6.1.76; HMM-score: 86.9)L-lysine 6-transaminase (TIGR03251; EC 2.6.1.36; HMM-score: 64.8)Central intermediary metabolism Other 4-aminobutyrate aminotransferase (TIGR00699; EC 2.6.1.19; HMM-score: 43.3)
- TheSEED :
- Glutamate-1-semialdehyde 2,1-aminomutase (EC 5.4.3.8)
- PFAM: PLP_aminotran (CL0061) Aminotran_3; Aminotransferase class-III (PF00202; HMM-score: 243.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: pyridoxal 5'-phosphate
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.033815
- TAT(Tat/SPI): 0.00861
- LIPO(Sec/SPII): 0.003893
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHGAYLYDVDGNKFIDYLQAYGPIIAGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKLRDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGPSQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMPQPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPIGGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQLEEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQGINLAPSKFEAWFLTTEHTEEDIQQTLKAADYAFSQMK
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SA1984 (asp23) alkaline shock protein 23 [1] (data from MRSA252) SA0727 (gap) glyceraldehyde-3-phosphate dehydrogenase [1] (data from MRSA252) SA1305 (hu) DNA-binding protein II [1] (data from MRSA252) SA0730 (pgm) phosphoglyceromutase [1] (data from MRSA252) SA2035 (rplE) 50S ribosomal protein L5 [1] (data from MRSA252) SA2029 (rplO) 50S ribosomal protein L15 [1] (data from MRSA252) SA2041 (rpsC) 30S ribosomal protein S3 [1] (data from MRSA252) SA0107 (spa) immunoglobulin G binding protein A [1] (data from MRSA252) SA0506 (tuf) elongation factor Tu [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)