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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS09645 [old locus tag: SA1681 ]
  • pan locus tag?: SAUPAN004844000
  • symbol: SA_RS09645
  • pan gene symbol?: gsaB
  • synonym:
  • product: glutamate-1-semialdehyde 2,1-aminomutase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS09645 [old locus tag: SA1681 ]
  • symbol: SA_RS09645
  • product: glutamate-1-semialdehyde 2,1-aminomutase
  • replicon: chromosome
  • strand: +
  • coordinates: 1926659..1927948
  • length: 1290
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (1926659..1927948) NCBI
  • BioCyc: SA_RS09645 BioCyc
  • MicrobesOnline: see SA1681

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAATTTTAGTGAAAGTGAACGTTTACAACAACTTTCAAACGAATATATTCTAGGCGGT
    GTCAATTCCCCTTCTCGTTCTTATAAAGCTGTAGGAGGCGGTGCACCTGTTGTTATGAAA
    GAAGGACACGGTGCATATTTATATGATGTCGATGGCAATAAATTTATTGATTACCTTCAA
    GCATACGGTCCAATTATTGCGGGGCATGCACATCCTCATATTACTAAAGCAATTCAAGAA
    CAAGCAGCCAAAGGTGTTTTATTTGGTACACCGACTGAACTAGAAATTGAATTCAGCAAA
    AAATTACGTGATGCAATTCCATCTCTTGAGAAAATTCGCTTTGTAAATTCTGGAACAGAA
    GCAGTCATGACAACAATTCGTGTTGCACGTGCATATACTAAAAGAAATAAAATTATAAAA
    TTTGCTGGATCTTATCATGGCCATTCTGATTTAGTATTGGTTGCAGCAGGTAGTGGCCCA
    TCTCAGCTCGGTTCTCCAGACTCAGCTGGTGTTCCAGAAAGCGTCGCACGTGAAGTCATT
    ACTGTACCTTTCAATGATATTAACGCATATAAAGAAGCAATTGAATTTTGGGGTGATGAA
    ATTGCCGCAGTATTAGTAGAACCAATTGTTGGTAACTTTGGAATGGTAATGCCTCAACCT
    GGATTTTTAGAAGAGGTTAATGAAATTTCGCATAACAATGGGACACTAGTGATTTATGAT
    GAAGTAATTACTGCATTCCGTTTCCATTACGGTGCCGCTCAAGATTTATTAGGTGTTATC
    CCTGATTTAACTGCATTTGGTAAAATTGTTGGCGGTGGTTTACCAATTGGAGGCTATGGT
    GGACGTCAAGATATTATGGAACAAGTAGCACCTCTAGGACCTGCATATCAAGCTGGTACA
    ATGGCTGGTAACCCGTTATCTATGAAAGCAGGTATTGCATTACTCGAAGTACTAGAACAA
    GACGGTGTTTATGAAAAATTAGACAGCTTAGGCCAACAACTAGAAGAAGGTTTACTTAAA
    TTAATCGAAAAACATAATATCACAGCTACAATTAATCGTATTTATGGATCTTTAACATTG
    TACTTTACAGATGAAAAAGTCACACATTATGATCAAGTTGAACATTCTGACGGCGAAGCG
    TTCGGTAAATTTTTCAAATTAATGTTAAATCAAGGTATCAATTTAGCACCTTCTAAGTTT
    GAAGCTTGGTTCTTAACAACTGAACATACAGAAGAAGATATTCAACAAACTTTAAAAGCT
    GCAGACTATGCTTTTAGTCAAATGAAATAA
    60
    120
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    540
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    840
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    960
    1020
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    1140
    1200
    1260
    1290

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS09645 [old locus tag: SA1681 ]
  • symbol: SA_RS09645
  • description: glutamate-1-semialdehyde 2,1-aminomutase
  • length: 429
  • theoretical pI: 4.83919
  • theoretical MW: 46725.6
  • GRAVY: -0.11352

⊟Function[edit | edit source]

  • reaction:
    EC 5.4.3.8?  ExPASy
    Glutamate-1-semialdehyde 2,1-aminomutase (S)-4-amino-5-oxopentanoate = 5-aminolevulinate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamate-1-semialdehyde-2,1-aminomutase (TIGR00713; EC 5.4.3.8; HMM-score: 548)
    and 10 more
    transaminase, acetylornithine/succinylornithine family (TIGR00707; HMM-score: 175)
    Metabolism Central intermediary metabolism Other 4-aminobutyrate transaminase (TIGR00700; EC 2.6.1.19; HMM-score: 157.9)
    ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 155.6)
    Metabolism Energy metabolism Amino acids and amines succinylornithine transaminase family (TIGR03246; EC 2.6.1.81; HMM-score: 133.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin adenosylmethionine-8-amino-7-oxononanoate transaminase (TIGR00508; EC 2.6.1.62; HMM-score: 129.2)
    Metabolism Central intermediary metabolism Polyamine biosynthesis putrescine aminotransferase (TIGR03372; EC 2.6.1.82; HMM-score: 111.7)
    Metabolism Central intermediary metabolism Other 2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 108.1)
    Cellular processes Cellular processes Adaptations to atypical conditions diaminobutyrate--2-oxoglutarate aminotransferase (TIGR02407; EC 2.6.1.76; HMM-score: 86.9)
    L-lysine 6-transaminase (TIGR03251; EC 2.6.1.36; HMM-score: 64.8)
    Metabolism Central intermediary metabolism Other 4-aminobutyrate aminotransferase (TIGR00699; EC 2.6.1.19; HMM-score: 43.3)
  • TheSEED: see SA1681
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_3; Aminotransferase class-III (PF00202; HMM-score: 243.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.033815
    • TAT(Tat/SPI): 0.00861
    • LIPO(Sec/SPII): 0.003893
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHGAYLYDVDGNKFIDYLQAYGPIIAGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKLRDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGPSQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMPQPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPIGGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQLEEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQGINLAPSKFEAWFLTTEHTEEDIQQTLKAADYAFSQMK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SA_RS00690immunoglobulin G-binding protein A  [1] (data from MRSA252)
    SA_RS02960elongation factor Tu  [1] (data from MRSA252)
    SA_RS04140aldehyde dehydrogenase  [1] (data from MRSA252)
    SA_RS041552,3-bisphosphoglycerate-independent phosphoglycerate mutase  [1] (data from MRSA252)
    SA_RS07385DNA-binding protein HU  [1] (data from MRSA252)
    SA_RS11430Asp23/Gls24 family envelope stress response protein  [1] (data from MRSA252)
    SA_RS1167050S ribosomal protein L15  [1] (data from MRSA252)
    SA_RS1170550S ribosomal protein L5  [1] (data from MRSA252)
    SA_RS1173530S ribosomal protein S3  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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