PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1987 [new locus tag: SA_RS11445 ]
• pan locus tag^?: SAUPAN005581000
• symbol: SA1987
• pan gene symbol^?: opuD2
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1987 [new locus tag: SA_RS11445 ]
• symbol: SA1987
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2257131..2258693
• length: 1563
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124903 NCBI
• RefSeq: NP_375298 NCBI
• BioCyc: see SA_RS11445
• MicrobesOnline: 104324 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  121
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  361
  421
  481
  541
  601
  661
  721
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  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  
  ATGAAAAATAAATCAGTAAGTCTAGTGTTTTGGGTATCTTTAGTGATATGTACGATATTT
  GTTGCTTTTGGAGCAATATTTCCGAAACAATTAGAAAAACTCACGCAAAACATTACTACA
  TTTATTGCGCTACATTTTTCTTGGTATTATTTATTGCTCGTTTTAGTTATTTTATTTGTC
  TGTGTGTATATTTTATTTTCGAGATACGCAAACATTACATTAGGTGAAGAAGGCGAAGAT
  CCTGAATTCTCATTGCCGTCTTGGTTTGCTATGTTATTTAGTGCTGGTATGGGAATAGGA
  CTTGTGTTCTGGACAACGGCAGAACCAATCAGTCATGCCTTCAAATTAACGCCTATTCAT
  AAAGCTGGCACGCAATCTGCAATTAATGATGCAATGCAGTTTTCATTTTTCCATTGGGGG
  ATTCATGCATGGGCAGTCTATGGTATTGTCGCGCTTGTGTTTGCATATTTTAGCTTTCAT
  AAAGGATATCCTGGCTTAGTCAGCGCTACATTGACGCCTTTATTAGGTGAAAAGGCAATG
  CGTGGACCTTTAGGTGGTGCCATTGATGTATTGGCAGTCATAGCTACAGTAACAGGCGTT
  GCTGCAACATTAGGTTTCGGTGCATTGCAAATAAACGAAGGTTTACATTTTTTATTTAAT
  GTACCGAGTAACTTCACGATGCAAGTAATCTTGATTGTCATTGCAACCATATTATTTACT
  TGGTCAGCATGGTCAGGTATCGATAAAGGTATCAAAACGTTGAGTAATATCAACATGTTA
  CTTGCTTTTGTAGTACTTATTGGTCTTTTTATTGTTGGGCCAACGCTTTACATTTTAAAT
  ACATTTACAAATGGTCTAGGTAATTATATTGCAAATTTCTTTAGTATGAGTTTACGTATT
  CCGTCAGGCGGTCAAAAATTCCAATGGTTACAAAATTGGACAATCTTTTACTGGGCATGG
  TGGATATCTTGGGCACCATTTGTAGGTATATTTATTGCACGTGTGTCAAAAGGACGTACG
  ATTAAAGAATTTATTCTAGGTGTACTTTTTGTCCCGGCGTTAGTTTGCTTTATATTCTTT
  GCGGTATTCGGCGCGTCAGCAATTTACTTACAAGATAATCATATTGCCGATATTGCTAAA
  GCAGCGACAGAAACAGCAACTTTTGCAACGCTACAACATTACCCACTAGGTTTTGTATTA
  AGCTTAATTACTTTACTTGTTATTATGATTTTCTTTGTTACATCAGCGGATTCGGCAACT
  TATGTTTTAGGTATGTTGAGTTCAAGAGGTGATATTAATCCGAAGTCATTTGTCAAAGTG
  AGTTGGGGTATCATTATGGCATTATTTGCAATTATCATGATCTACACAGGTGGTACTCAA
  GCGATTCAAAATTTATTAATCATTGCTGCGTTACCATTTTCCGTGGTCATTATAGCGATG
  ATATGGTCGCTTTTAAAATCTTTAAGTGAAGAAAAACCTAGAAATAGTAATAAGGTTTTA
  ATTAAACATCGTGACCCAGATATTTTAGAATATCGTTCTTCAAAACATATTGACGAAAAT
  TAA
  60
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  1260
  1320
  1380
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  1500
  1560
  1563
  

⊟Protein

⊟General

• locus tag: SA1987 [new locus tag: SA_RS11445 ]
• symbol: SA1987
• description: hypothetical protein
• length: 520
• theoretical pI: 9.11697
• theoretical MW: 57690.8
• GRAVY: 0.78

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Other transporter, betaine/carnitine/choline transporter (BCCT) family (TIGR00842; HMM-score: 528.7)
• TheSEED  :
  • Betaine transporter OpuD
  Stress Response Osmotic stress Choline and Betaine Uptake and Betaine Biosynthesis  Glycine betaine transporter OpuD
• PFAM:
  APC (CL0062) BCCT; BCCT, betaine/carnitine/choline family transporter (PF02028; HMM-score: 626.3)
  and 1 more
  M6PR (CL0226) ATG27; Autophagy-related protein 27 (PF09451; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 12
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.017815
  • TAT(Tat/SPI): 0.000231
  • LIPO(Sec/SPII): 0.309842
• predicted transmembrane helices (TMHMM): 12

⊟Accession numbers

• GI: 15927765 NCBI
• RefSeq: NP_375298 NCBI
• UniProt: A0A0H3JNH0 UniProt

⊟Protein sequence

• MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFHKGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQHYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSRGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLLKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications