From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2176 [new locus tag: SACOL_RS11450 ]
  • pan locus tag?: SAUPAN005581000
  • symbol: opuD2
  • pan gene symbol?: opuD2
  • synonym:
  • product: BCCT family osmoprotectant transporter

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2176 [new locus tag: SACOL_RS11450 ]
  • symbol: opuD2
  • product: BCCT family osmoprotectant transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2256981..2258543
  • length: 1563
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGAAAAATAAATCAGTAAGTCTAGTGTTTTGGGTATCTTTAGTGATATGTACGATATTT
    GTTGCTTTTGGAGCAATATTTCCGAAACAGTTAGAAAAACTCACGCAAAACATTACTACA
    TTTATTGCGCTACATTTTTCTTGGTATTATTTATTGCTCGTTTTAGTTATTTTATTTGTC
    TGTGTGTATATTTTATTTTCGAGATACGCAAACATTACATTAGGTGAAGAAGGCGAAGAT
    CCTGAATTCTCATTGCCGTCTTGGTTTGCTATGTTATTTAGTGCTGGTATGGGAATAGGA
    CTTGTGTTCTGGACAACGGCAGAACCAATCAGTCATGCCTTCAAATTAACGCCTATTCAT
    AAAGCTGGCACGCAATCTGCAATTAATGATGCAATGCAGTTTTCATTTTTCCATTGGGGG
    ATTCATGCATGGGCAGTCTATGGTATTGTCGCGCTAGTGTTTGCATATTTTAGCTTTCAT
    AAAGGATATCCTGGCTTAGTCAGCGCTACATTGACGCCTTTATTAGGTGAAAAGGCAATG
    CGTGGACCTTTAGGTGGTGCCATTGATGTATTGGCAGTCATAGCTACAGTAACAGGCGTT
    GCTGCAACATTAGGTTTCGGTGCATTGCAAATAAACGAAGGTTTACATTTTTTATTTAAT
    GTACCGAGTAACTTCACGATGCAAGTAATCTTGATTGTCATTGCAACCATATTATTTACT
    TGGTCAGCATGGTCAGGTATCGATAAAGGTATCAAAACGTTGAGTAATATCAACATGTTA
    CTTGCTTTTGTAGTACTTATTGGTCTTTTTATTGTTGGGCCAACGCTATACATTTTAAAT
    ACATTTACGAATGGTCTAGGTAATTATATTGCAAATTTCTTTAGTATGAGTTTACGCATT
    CCGACAGGTGGTCAAAAATTCCAATGGTTACAAAATTGGACAATCTTTTACTGGGCATGG
    TGGATATCTTGGGCACCATTTGTAGGTATATTTATTGCACGTGTGTCAAAAGGACGTACG
    ATTAAAGAATTTATTCTAGGTGTACTTTTTGTCCCGGCGTTAGTTTGCTTTATATTCTTT
    GCGGTATTCGGCGCGTCAGCAATTTACTTACAAGATAATCATATTGCCGATATTGCTAAA
    GCTGCGACAGAAACAGCAACTTTTGCGACGCTACAACATTATCCGCTAGGTTTTGTATTA
    AGCTTAATTACTTTACTTGTTATTATGATTTTCTTTGTTACATCAGCGGATTCGGCAACT
    TATGTTTTAGGTATGTTGAGTTCAAGTGGTGATATTAATCCGAAGTCATTTGTCAAAGTG
    AGTTGGGGTATCATTATGGCATTATTTGCAATCATCATGATTTACACAGGTGGTACTCAA
    GCAATTCAAAATTTATTAATCATTGCTGCGTTACCATTTTCCGTGGTCATTATAGCGATG
    ATATGGTCGCTTATAAAATCTTTGAGTGAGGAGAAACCGAGAAATAGTAATAAGGTTTTA
    ATTAAACATCGTGACCCAGATATTTTAGAATATCGTTCTTCAAAACATATTGACGAAAAT
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1563

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2176 [new locus tag: SACOL_RS11450 ]
  • symbol: OpuD2
  • description: BCCT family osmoprotectant transporter
  • length: 520
  • theoretical pI: 8.91716
  • theoretical MW: 57635.7
  • GRAVY: 0.788654

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Other transporter, betaine/carnitine/choline transporter (BCCT) family (TIGR00842; HMM-score: 528.4)
  • TheSEED  :
    • Glycine betaine transporter OpuD
    Stress Response Osmotic stress Choline and Betaine Uptake and Betaine Biosynthesis  Glycine betaine transporter OpuD
  • PFAM:
    APC (CL0062) BCCT; BCCT, betaine/carnitine/choline family transporter (PF02028; HMM-score: 625.6)
    and 1 more
    M6PR (CL0226) ATG27; Autophagy-related protein 27 (PF09451; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.017815
    • TAT(Tat/SPI): 0.000231
    • LIPO(Sec/SPII): 0.309842
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIALHFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLVFWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFHKGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFLFNVPSNFTMQVILIVIATILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTLYILNTFTNGLGNYIANFFSMSLRIPTGGQKFQWLQNWTIFYWAWWISWAPFVGIFIARVSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQHYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSSGDINPKSFVKVSWGIIMALFAIIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLIKSLSEEKPRNSNKVLIKHRDPDILEYRSSKHIDEN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [3] [4]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  4. ↑ Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int J Med Microbiol: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SACOL2176&oldid=99748"