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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2071 [new locus tag: SA_RS11910 ]
  • pan locus tag?: SAUPAN005737000
  • symbol: moeB
  • pan gene symbol?: moeB
  • synonym:
  • product: molybdopterin biosynthesis protein moeB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2071 [new locus tag: SA_RS11910 ]
  • symbol: moeB
  • product: molybdopterin biosynthesis protein moeB
  • replicon: chromosome
  • strand: -
  • coordinates: 2331836..2332840
  • length: 1005
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGAGTCAAGAACGTTATTCAAGGCAAATTTTATTTAAACAAATAGGTGAAATAGGTCAA
    AGCAAAATAAATCAAAAATGTGCGTTGATTATTGGTATGGGCGCATTAGGTACACATGTG
    GCCGAAGGACTTGTTAGAGCAGGCATTGCTAAACTAATCATTGTTGATAGAGATTATATT
    GAATTTAGTAATTTACAAAGACAAACATTGTTTACTGAAGAAGATGCTTTGAAAATGATG
    CCTAAAGTGGTTGCAGCTAAAAAGCATTTGCTAGCGTTACGTAGTGATGTTGATATTGAT
    GGTTGTATTGCCCATGTGGATTATTATTTTTTGGAAACACATGGACAGGACGTTGACGTT
    ATTATTGATGCAACCGATAACTTTGAAACACGACAACTGATTAATGATTTTGCATATAAA
    CATCGTATACCTTGGATTTATGGTGGTGTTGTACAGAGTACATATACAGAAGCTGCATTT
    ATACCTGGTAAAACACCTTGCTTTAACTGTTTGGTACCACAATTGCCAGCATTAAATTTA
    ACATGTGATACAGTAGGGGTCATTCAACCTGCCGTGACGATGGCAACAAGTTTACAATTA
    AGAGATGCGATGAAAGTATTAACGGAACAACCAATTGACACAAAAATAACTTATGGCGAT
    ATTTGGGAAGGTAGTCATTATTCATTTGGTTTCAGTAAAATGCAACGTTCAGACTGTACA
    ACTTGTGGAGATGTACCAAGTTATCCGTATTTAAACAAGAATGAACAACGTTATGCAACA
    TTGTGTGGTAGAGACACTGTACAGTATGAAAATGCATCAATTACACACGACATTCTTGTT
    CAATTTTTAAAACAACATCAACTAAATTATCGCAGTAATTCGTATATGGTTATGTTTGAA
    TTTAAAGGACACCGCATTGTTGCTTTTAAAGGTGGAAGGTTTTTAATACATGGCATGACA
    CGCACATCAGATGCCACACATCTAATGAATTTATTGTTTGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1005

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2071 [new locus tag: SA_RS11910 ]
  • symbol: MoeB
  • description: molybdopterin biosynthesis protein moeB
  • length: 334
  • theoretical pI: 7.0759
  • theoretical MW: 37776.2
  • GRAVY: -0.12994

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis adenylyltransferase ThiF (TIGR02356; EC 2.7.7.73; HMM-score: 205.5)
    and 7 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin synthase sulfurylase MoeB (TIGR02355; HMM-score: 126.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine biosynthesis protein ThiF (TIGR02354; HMM-score: 67.2)
    ubiquitin-activating enzyme E1 (TIGR01408; HMM-score: 36.9)
    E1-like protein-activating enzyme Gsa7p/Apg7p (TIGR01381; HMM-score: 32.9)
    Cellular processes Cellular processes Pathogenesis thiazole/oxazole-forming peptide maturase, SagC family component (TIGR03603; EC 6.3.-.-; HMM-score: 29.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 20.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 15.1)
  • TheSEED  :
    • Molybdopterin-synthase adenylyltransferase (EC 2.7.7.80)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Molybdenum cofactor biosynthesis  Molybdopterin biosynthesis protein MoeB
  • PFAM:
    NADP_Rossmann (CL0063) ThiF; ThiF family (PF00899; HMM-score: 169.9)
    and 7 more
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 23.9)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 20.6)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 16.1)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 14.9)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 13.9)
    KR; KR domain (PF08659; HMM-score: 13)
    ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.066023
    • TAT(Tat/SPI): 0.024209
    • LIPO(Sec/SPII): 0.002925
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGLVRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDGCIAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKHRIPWIYGGVVQSTYTEAAFIPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITYGDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITHDILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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