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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2269 [new locus tag: SACOL_RS11930 ]
  • pan locus tag?: SAUPAN005737000
  • symbol: SACOL2269
  • pan gene symbol?: moeB
  • synonym:
  • product: molybdopterin biosynthesis MoeB protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2269 [new locus tag: SACOL_RS11930 ]
  • symbol: SACOL2269
  • product: molybdopterin biosynthesis MoeB protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2330575..2331579
  • length: 1005
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGAGTCAAGAACGTTATTCAAGGCAAATTTTATTTAAACAAATAGGTGAAATAGGTCAA
    AGCAAAATAAATCAAAAATGTGCGTTGATTATTGGTATGGGCGCATTAGGTACACATGTG
    GCCGAAGGACTTGTTAGAGCAGGCATTGCCAAACTAATCATTGTTGATAGAGATTATATT
    GAATTTAGTAATTTACAAAGACAAACATTGTTTACTGAAGAAGATGCTTTGAAAATGATG
    CCTAAGGTGGTTGCAGCTAAAAAGCATTTGCTAGCGTTACGTAGTGATGTTGATATTGAT
    GATTATATTGCCCATGTGGATTATTATTTTTTGGAAACACATGGACAGGACGTTGACGTT
    ATTATTGATGCAACCGATAACTTTGAAACACGACAACTGATTAATGATTTTGCATATAAA
    TATCGTATACCTTGGATTTATGGTGGTGTTGTACAGAGTACATATACAGAAGCTGCATTT
    ATACCTGGTAAAACACCTTGCTTTAACTGTTTGGTACCACAATTGCCAGCATTAAATTTA
    ACATGTGATACAGTAGGGGTCATTCAACCTGCCGTGACGATGGCAACAAGTTTACAATTA
    AGAGATGCGATGAAAGTATTAACGGAACAACCAATTGACACAAAAATAACTTATGGCGAT
    ATTTGGGAAGGTAGTCATTATTCATTTGGTTTCAGTAAAATGCAACGTTCAGACTGTACA
    ACTTGTGGAGATGTACCAAGTTATCCGTATTTAAACAAGAATGAACAACGTTATGCAACA
    TTGTGTGGTAGAGACACTGTACAGTATGAAAATGCATCAATTACACACGACATTCTTGTT
    CAATTTTTAAAACAACATCAGTTAAATTATCGCAGTAATTCGTATATGGTTATGTTTGAA
    TTTAAAGGACACCGCATTGTTGCTTTTAAAGGTGGAAGGTTTTTAATACATGGCATGACA
    CGCACATCAGATGCCACACATCTAATGAATTTATTGTTTGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1005

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2269 [new locus tag: SACOL_RS11930 ]
  • symbol: SACOL2269
  • description: molybdopterin biosynthesis MoeB protein
  • length: 334
  • theoretical pI: 6.84183
  • theoretical MW: 37920.3
  • GRAVY: -0.14491

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis adenylyltransferase ThiF (TIGR02356; EC 2.7.7.73; HMM-score: 204.8)
    and 8 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin synthase sulfurylase MoeB (TIGR02355; HMM-score: 124.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine biosynthesis protein ThiF (TIGR02354; HMM-score: 67)
    ubiquitin-activating enzyme E1 (TIGR01408; HMM-score: 37.1)
    E1-like protein-activating enzyme Gsa7p/Apg7p (TIGR01381; HMM-score: 32.8)
    Cellular processes Cellular processes Pathogenesis thiazole/oxazole-forming peptide maturase, SagC family component (TIGR03603; EC 6.3.-.-; HMM-score: 32.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 21.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 15.1)
    PRTRC system ThiF family protein (TIGR03736; HMM-score: 11.2)
  • TheSEED  :
    • Molybdopterin biosynthesis protein MoeB
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Molybdenum cofactor biosynthesis  Molybdopterin biosynthesis protein MoeB
  • PFAM:
    NADP_Rossmann (CL0063) ThiF; ThiF family (PF00899; HMM-score: 172.4)
    and 9 more
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 24.3)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 21.1)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 16)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 14.9)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 14.4)
    KR; KR domain (PF08659; HMM-score: 13.5)
    ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 12.6)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 11.9)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 10.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.066023
    • TAT(Tat/SPI): 0.024209
    • LIPO(Sec/SPII): 0.002925
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGLVRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDYIAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAFIPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITYGDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITHDILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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