PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2227 [new locus tag: SAUSA300_RS12300 ]
• pan locus tag^?: SAUPAN005737000
• symbol: moeB
• pan gene symbol^?: moeB
• synonym:
• product: molybdopterin biosynthesis protein B

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2227 [new locus tag: SAUSA300_RS12300 ]
• symbol: moeB
• product: molybdopterin biosynthesis protein B
• replicon: chromosome
• strand: -
• coordinates: 2394442..2395446
• length: 1005
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915094 NCBI
• RefSeq: YP_494862 NCBI
• BioCyc: see SAUSA300_RS12300
• MicrobesOnline: 1293742 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  GTGAGTCAAGAACGTTATTCAAGGCAAATTTTATTTAAACAAATAGGTGAAATAGGTCAA
  AGCAAAATAAATCAAAAATGTGCGTTGATTATTGGTATGGGCGCATTAGGTACACATGTG
  GCCGAAGGACTTGTTAGAGCAGGCATTGCCAAACTAATCATTGTTGATAGAGATTATATT
  GAATTTAGTAATTTACAAAGACAAACATTGTTTACTGAAGAAGATGCTTTGAAAATGATG
  CCTAAGGTGGTTGCAGCTAAAAAGCATTTGCTAGCGTTACGTAGTGATGTTGATATTGAT
  GATTATATTGCCCATGTGGATTATTATTTTTTGGAAACACATGGACAGGACGTTGACGTT
  ATTATTGATGCAACCGATAACTTTGAAACACGACAACTGATTAATGATTTTGCATATAAA
  TATCGTATACCTTGGATTTATGGTGGTGTTGTACAGAGTACATATACAGAAGCTGCATTT
  ATACCTGGTAAAACACCTTGCTTTAACTGTTTGGTACCACAATTGCCAGCATTAAATTTA
  ACATGTGATACAGTAGGGGTCATTCAACCTGCCGTGACGATGGCAACAAGTTTACAATTA
  AGAGATGCGATGAAAGTATTAACGGAACAACCAATTGACACAAAAATAACTTATGGCGAT
  ATTTGGGAAGGTAGTCATTATTCATTTGGTTTCAGTAAAATGCAACGTTCAGACTGTACA
  ACTTGTGGAGATGTACCAAGTTATCCGTATTTAAACAAGAATGAACAACGTTATGCAACA
  TTGTGTGGTAGAGACACTGTACAGTATGAAAATGCATCAATTACACACGACATTCTTGTT
  CAATTTTTAAAACAACATCAGTTAAATTATCGCAGTAATTCGTATATGGTTATGTTTGAA
  TTTAAAGGACACCGCATTGTTGCTTTTAAAGGTGGAAGGTTTTTAATACATGGCATGACA
  CGCACATCAGATGCCACACATCTAATGAATTTATTGTTTGGATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2227 [new locus tag: SAUSA300_RS12300 ]
• symbol: MoeB
• description: molybdopterin biosynthesis protein B
• length: 334
• theoretical pI: 6.84183
• theoretical MW: 37920.3
• GRAVY: -0.14491

⊟Function

• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis adenylyltransferase ThiF (TIGR02356; EC 2.7.7.73; HMM-score: 204.8)
  and 8 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin synthase sulfurylase MoeB (TIGR02355; HMM-score: 124.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine biosynthesis protein ThiF (TIGR02354; HMM-score: 67)
  ubiquitin-activating enzyme E1 (TIGR01408; HMM-score: 37.1)
  E1-like protein-activating enzyme Gsa7p/Apg7p (TIGR01381; HMM-score: 32.8)
  Cellular processes Cellular processes Pathogenesis thiazole/oxazole-forming peptide maturase, SagC family component (TIGR03603; EC 6.3.-.-; HMM-score: 32.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 21.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 15.1)
  PRTRC system ThiF family protein (TIGR03736; HMM-score: 11.2)
• TheSEED  :
  • Molybdopterin biosynthesis protein MoeB
  Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Molybdenum cofactor biosynthesis  Molybdopterin biosynthesis protein MoeB
• PFAM:
  NADP_Rossmann (CL0063) ThiF; ThiF family (PF00899; HMM-score: 172.4)
  and 9 more
  Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 24.3)
  NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 21.1)
  TrkA_N; TrkA-N domain (PF02254; HMM-score: 16)
  2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 14.9)
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 14.4)
  KR; KR domain (PF08659; HMM-score: 13.5)
  ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 12.6)
  UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 11.9)
  Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 10.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.066023
  • TAT(Tat/SPI): 0.024209
  • LIPO(Sec/SPII): 0.002925
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160503 NCBI
• RefSeq: YP_494862 NCBI
• UniProt: A0A0H2XGQ6 UniProt

⊟Protein sequence

• MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGLVRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDYIAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKYRIPWIYGGVVQSTYTEAAFIPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITYGDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITHDILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: moaB < moeB

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications