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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2216 [new locus tag: SA_RS12725 ]
  • pan locus tag?: SAUPAN005971000
  • symbol: SA2216
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2216 [new locus tag: SA_RS12725 ]
  • symbol: SA2216
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2487158..2488891
  • length: 1734
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGTTTCAAATTACATTTAAAATTTTAAACTGGATAAGACCATATAAAGCAAGAATGATT
    TTAGGATTTAGCATGTCATTTTTAAATGCTATTTTTATTGCGTTACCTATCTTTTTAGCT
    GCAAAAATATTTAATAATGTGCTATCTCATAAACCTATTTATATGAAAGATATATTGAAT
    GTTGTAATTATCATGGTTTTATTAGTGATTGGACGATTTATTACAGCATATTTCAAAAGC
    AAAAGCCATGAGAGCATCGCTTATGAAATGAGTGCGAAAGAACGTTTAGATATAGGGGAT
    AAATTGAAAAATGTAAGGTTAGGTTATTTTAATTCGCATCATTCAAATGAGTTAACAACA
    ATAGTAACAACAGATTTAACATTTCTAGAAAACTTTGCCATGAAAATGGTAGATATTGTT
    GTTAATGGATACATATTAATTACAGTACTCATATTGTCTCTACTTGTAGTTTCGTGGCAA
    GTATCATTATTAGCATGCATTGGCGTATTACTATCATTTTTTGCGATTCAATTATTAGAA
    AGAAAGAGTCGACAAAATGCGCCAGCGTATCATAATGTACAAAACCAATTAGTGGAAAAG
    GTATTGGAGGTTATTCGTGGTATTCAAGTAATAAAATCATTCGCGAAAGAAAATACGAGT
    CTTAAAAGTTTTAACCAAGCAGTCAATGAAAGTAAACGTATAAATACAAAAATAGAAATG
    CAATATATCCCATTCAATTTATTGCATTTACTTAGTTTAAAAGTTGTTTCAATAATGATT
    GTATTAGTTGCATGCTTATTATATATGAATCATAGTATTGATTTACCTACCCTTATTATG
    ATTTCAATTTTTTCATTTGTGATATTTGATAGTGTTGAAAATATTAATAGTGCTGCACAC
    GTACTTGAAATGATAGATATGACGATAGATGATATTGAAAAGATAAAAAATGCTCCAGAA
    CTGGATGAGAATGGAAAAAATTTGACGATTAAAAATGAAAATATCGCTTTTCAAAACGTG
    AACTTTTCATATGATGATAAACAAGTGATAAAGAATGTGAATTTTGAGATACCTACACAA
    ACATCAACAGCAATAATTGGACCTTCAGGAAGTGGGAAATCTACATTATGTCACTTACTC
    TTGCGCTTTTATGATATCGATGATGGAAATATTCGCATCGATGGTGTTGATATTAAAGAT
    ATGACATTAAGTACGTTAATGTCGAAAATTAGTGCAGTATTTCAAAAGGTGTATTTATTT
    AATGATACGATTGAAAATAACATATTGTTTGGCAATCCAGGTGCAACGAAAGAAGAAATT
    ATTCGTGCAGCGAAGCAAGCATGTTGCCACGACTTCATCATGTCATTACCTGAGGGATAT
    CAAACAATGCTAAATGAAAAAGGTAGTAATTTATCTGGCGGAGAAAAGCAAAGGATTTCT
    ATTGCTAGGGCGATATTAAAAGATGCACCAATAATTATTTTAGATGAAGCAACTGCAAGT
    ATTGACCCTGAAAATGAACAGCTGATTCAAACGGCAATTAATGAATTAAGTAAAGGCAAA
    ACAGTAATTACAATTGCACATAAACTTGAAACTATTAAAAATGCAGATCAGATTATAGTG
    CTCAATGAAGGTGAAATAATTCAAAAAGGTAGTCATGATGAATTAATTCGAAAACCAGGA
    ATGTATCAAGACTTTATAAGAATAAAAAGTAAATCAGCAGGCTGGAAATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1734

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2216 [new locus tag: SA_RS12725 ]
  • symbol: SA2216
  • description: hypothetical protein
  • length: 577
  • theoretical pI: 7.65215
  • theoretical MW: 65076.8
  • GRAVY: 0.192028

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 366.4)
    Metabolism Transport and binding proteins Other lipid A export permease/ATP-binding protein MsbA (TIGR02203; HMM-score: 366.4)
    ABC transporter, permease/ATP-binding protein (TIGR02204; HMM-score: 345.7)
    Metabolism Transport and binding proteins Other antigen peptide transporter 2 (TIGR00958; HMM-score: 324.7)
    and 72 more
    thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 292.2)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01846; HMM-score: 288.7)
    Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 276.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, peptidase/ATP-binding protein (TIGR03796; HMM-score: 276.9)
    Cellular processes Cellular processes Biosynthesis of natural products NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 261.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines NHLM bacteriocin system ABC transporter, ATP-binding protein (TIGR03797; HMM-score: 261.9)
    Cellular processes Cellular processes Pathogenesis type I secretion system ATPase (TIGR03375; HMM-score: 257.7)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR03375; HMM-score: 257.7)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids glucan exporter ATP-binding protein (TIGR01192; EC 3.6.3.42; HMM-score: 253.4)
    thiol reductant ABC exporter, CydC subunit (TIGR02868; HMM-score: 236.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking ABC-type bacteriocin transporter (TIGR01193; HMM-score: 230.1)
    Genetic information processing Protein fate Protein modification and repair ABC-type bacteriocin transporter (TIGR01193; HMM-score: 230.1)
    Metabolism Transport and binding proteins Other ABC-type bacteriocin transporter (TIGR01193; HMM-score: 230.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type I secretion system ATPase (TIGR01842; HMM-score: 203.3)
    Metabolism Transport and binding proteins Other multi drug resistance-associated protein (MRP) (TIGR00957; HMM-score: 150.9)
    Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04520; EC 3.6.3.-; HMM-score: 150.8)
    Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 143.2)
    Metabolism Transport and binding proteins Anions phosphate ABC transporter, ATP-binding protein (TIGR00972; EC 3.6.3.27; HMM-score: 139.9)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids ABC transporter, ATP-binding subunit, PQQ-dependent alcohol dehydrogenase system (TIGR03864; HMM-score: 127.1)
    Metabolism Transport and binding proteins Amino acids, peptides and amines glycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 125.9)
    Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 125.5)
    Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 125.5)
    Metabolism Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 125)
    Metabolism Transport and binding proteins Anions sulfate ABC transporter, ATP-binding protein (TIGR00968; EC 3.6.3.25; HMM-score: 110.7)
    Cellular processes Cellular processes Cell division cell division ATP-binding protein FtsE (TIGR02673; HMM-score: 106.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtE (TIGR03410; HMM-score: 105.7)
    Metabolism Transport and binding proteins Anions cystic fibrosis transmembrane conductor regulator (CFTR) (TIGR01271; EC 3.6.3.49; HMM-score: 102.8)
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, ATP-binding protein (TIGR02142; EC 3.6.3.29; HMM-score: 99.9)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 98.4)
    Metabolism Transport and binding proteins Other LPS export ABC transporter ATP-binding protein (TIGR04406; HMM-score: 98.4)
    Metabolism Transport and binding proteins Amino acids, peptides and amines polyamine ABC transporter, ATP-binding protein (TIGR01187; EC 3.6.3.31; HMM-score: 98)
    Metabolism Transport and binding proteins Other thiamine ABC transporter, ATP-binding protein (TIGR01277; EC 3.6.3.-; HMM-score: 96.5)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking lipoprotein releasing system, ATP-binding protein (TIGR02211; EC 3.6.3.-; HMM-score: 95.8)
    Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, ATP-binding protein (TIGR03265; HMM-score: 94.3)
    Metabolism Transport and binding proteins Other daunorubicin resistance ABC transporter, ATP-binding protein (TIGR01188; HMM-score: 93.8)
    ABC exporter ATP-binding subunit, DevA family (TIGR02982; HMM-score: 91.9)
    ectoine/hydroxyectoine ABC transporter, ATP-binding protein EhuA (TIGR03005; HMM-score: 90.6)
    D-methionine ABC transporter, ATP-binding protein (TIGR02314; EC 3.6.3.-; HMM-score: 89.3)
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikE (TIGR02769; EC 3.6.3.24; HMM-score: 88.4)
    Metabolism Transport and binding proteins Cations and iron carrying compounds nickel import ATP-binding protein NikD (TIGR02770; HMM-score: 87.6)
    proposed F420-0 ABC transporter, ATP-binding protein (TIGR03873; HMM-score: 86.9)
    Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtD (TIGR03411; HMM-score: 86.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines cyclic peptide transporter (TIGR01194; HMM-score: 83.5)
    Metabolism Transport and binding proteins Other cyclic peptide transporter (TIGR01194; HMM-score: 83.5)
    Metabolism Transport and binding proteins Cations and iron carrying compounds cobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 82.5)
    Cellular processes Cellular processes Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 78.8)
    Metabolism Transport and binding proteins Other nodulation ABC transporter NodI (TIGR01288; HMM-score: 78.8)
    phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 78.8)
    Metabolism Energy metabolism Methanogenesis methyl coenzyme M reductase system, component A2 (TIGR03269; HMM-score: 77.7)
    Metabolism Transport and binding proteins Amino acids, peptides and amines choline ABC transporter, ATP-binding protein (TIGR03415; HMM-score: 77.5)
    gliding motility-associated ABC transporter ATP-binding subunit GldA (TIGR03522; HMM-score: 77.5)
    Metabolism Transport and binding proteins Other pigment precursor permease (TIGR00955; HMM-score: 76.4)
    2-aminoethylphosphonate ABC transport system, ATP-binding component PhnT (TIGR03258; HMM-score: 76.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly ATPase SufC (TIGR01978; HMM-score: 69.6)
    lantibiotic protection ABC transporter, ATP-binding subunit (TIGR03740; HMM-score: 69.3)
    ATP-binding cassette protein, ChvD family (TIGR03719; HMM-score: 66.6)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids D-xylose ABC transporter, ATP-binding protein (TIGR02633; EC 3.6.3.17; HMM-score: 64.9)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 58.4)
    Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 58.4)
    Metabolism Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, ATP-binding subunit (TIGR03771; HMM-score: 57)
    Metabolism Transport and binding proteins Anions nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 56.5)
    Metabolism Transport and binding proteins Other nitrate ABC transporter, ATP-binding proteins C and D (TIGR01184; HMM-score: 56.5)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit A (TIGR00630; EC 3.1.25.-; HMM-score: 55)
    Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids peroxysomal long chain fatty acyl transporter (TIGR00954; HMM-score: 52.8)
    Metabolism Transport and binding proteins Other rim ABC transporter (TIGR01257; HMM-score: 44.7)
    Metabolism Transport and binding proteins Other pleiotropic drug resistance family protein (TIGR00956; HMM-score: 42.6)
    Metabolism Central intermediary metabolism Phosphorus compounds phosphonate C-P lyase system protein PhnK (TIGR02323; HMM-score: 34.5)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02168; HMM-score: 29.2)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02168; HMM-score: 29.2)
    Genetic information processing Protein synthesis Translation factors ribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 16.8)
    Cellular processes Cellular processes Cell division chromosome segregation protein SMC (TIGR02169; HMM-score: 14.2)
    Genetic information processing DNA metabolism Chromosome-associated proteins chromosome segregation protein SMC (TIGR02169; HMM-score: 14.2)
  • TheSEED  :
    • Heterodimeric efflux ABC transporter, permease/ATP-binding subunit 2
  • PFAM:
    P-loop_NTPase (CL0023) ABC_tran; ABC transporter (PF00005; HMM-score: 111)
    and 20 more
    ABC_membrane (CL0241) ABC_membrane; ABC transporter transmembrane region (PF00664; HMM-score: 60.9)
    P-loop_NTPase (CL0023) SMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 47.3)
    AAA_22; AAA domain (PF13401; HMM-score: 26.7)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 22.7)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 21.6)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 21.4)
    AAA_24; AAA domain (PF13479; HMM-score: 21)
    AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 18.6)
    AAA_18; AAA domain (PF13238; HMM-score: 18)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 17.2)
    IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 15.8)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 15)
    SbcCD_C; Putative exonuclease SbcCD, C subunit (PF13558; HMM-score: 14.4)
    ABC_ATPase; Predicted ATPase of the ABC class (PF09818; HMM-score: 14.2)
    AAA_23; AAA domain (PF13476; HMM-score: 14)
    NTPase_1; NTPase (PF03266; HMM-score: 13.3)
    Cytidylate_kin; Cytidylate kinase (PF02224; HMM-score: 12.6)
    DUF815; Protein of unknown function (DUF815) (PF05673; HMM-score: 12.4)
    AAA_14; AAA domain (PF13173; HMM-score: 12.3)
    no clan defined SP_C-Propep; Surfactant protein C, N terminal propeptide (PF08999; HMM-score: 7.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009781
    • TAT(Tat/SPI): 0.000523
    • LIPO(Sec/SPII): 0.009187
  • predicted transmembrane helices (TMHMM): 5

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIFNNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKLKNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDIVVNGYILITVLILSLLVVSWQVSLLACIGVLLSFFAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKENTSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLPTLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNENIAFQNVNFSYDDKQVIKNVNFEIPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIRIDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRAAKQACCHDFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQLIQTAINELSKGKTVITIAHKLETIKNADQIIVLNEGEIIQKGSHDELIRKPGMYQDFIRIKSKSAGWKL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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