PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2220 [new locus tag: SA_RS12750 ]
• pan locus tag^?: SAUPAN005980000
• symbol: SA2220
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2220 [new locus tag: SA_RS12750 ]
• symbol: SA2220
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 2492277..2493419
• length: 1143
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125148 NCBI
• RefSeq: NP_375543 NCBI
• BioCyc: see SA_RS12750
• MicrobesOnline: 104569 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  
  ATGCAATTACAAAAAATTGTCATCGCTCCTGACTCATTTAAGGAAAGTATGACCGCACAG
  CAAGTTGGCAATATTATAAAACAGGCATTTACTAATGTTTATGGGAATACCCTTCATTAT
  GATATCATTCCGATGGCTGATGGTGGTGAAGGTACCACAGATGCTTTAATGCATGCAACA
  GGTGCCACTAAGTATACAGTCATCGTTAATGACCCTTTAATGCGACCTATTGAAGCATGT
  TATGCACGCGCAGACGAACAACAAATTGCAATTATTGAAATGGCGGCAGCGTCAGGTTTG
  GATTTATTAGAAAAAGAGGAACGTAATCCTTTATACACATCATCATATGGTACCGGTGAA
  CTAATTAAAGATGCATTAAATCATGGTGCTAAGACCATTATTTTAGGGATTGGTGGCAGT
  GCAACAAATGATGGTGGTACAGGTATGCTAAGTGCACTAGGCGTAAAGTTTACTGATGTA
  AACGGGGACTTATTACAAATGAATGGTGCTAATCTTGCTCACATTGCACAAATCGATATA
  ACCAATCTAGATTCGCGATTAAAAGAGGTGACCTTTAAAGTGGCCTGTGATGTTTCAAAT
  CCTTTATTGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATGCA
  AAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGC
  ACAGGAAAGTCCGTTAATCAAATACCAGGTTCTGGTGCAGCTGGCGGTATGGGCGCAGCA
  TTATTAGCGTTTTGTGAGACAACTTTAACAAAAGGTATTGATGTCGTCTTTGACATTACA
  GATTTTCATCAAAGAATTAAAGATGCAGACCTCGTTATTACTGGAGAAGGACGCATGGAT
  TATCAGACCATCTTTGGTAAAACACCCGTAGGCGTTGCGTTAGCTGCAAAACAATATCAT
  ATTCCTGTCATCGCGATTTGTGGCAGTCTAGGCGAAAATTATCAACATGTTTACGATTTC
  GGTATTGATAGTGCCTATTCTATAATCTCTTCACCTAGCACTTTAGAAGATGTCCTACAA
  AATAGCGAACAAAATTTATTAAACACTGCAACTGACATTGCTCGTATTCTGAAATTACAA
  TAA
  60
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  180
  240
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  360
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  720
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  840
  900
  960
  1020
  1080
  1140
  1143
  

⊟Protein

⊟General

• locus tag: SA2220 [new locus tag: SA_RS12750 ]
• symbol: SA2220
• description: hypothetical protein
• length: 380
• theoretical pI: 4.76396
• theoretical MW: 40660.1
• GRAVY: -0.02

⊟Function

• TIGRFAM:
  Metabolism Energy metabolism Other glycerate kinase (TIGR00045; EC 2.7.1.31; HMM-score: 576.3)
• TheSEED  :
  • Glycerate kinase (EC 2.7.1.31)
  Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  Glycerate kinase (EC 2.7.1.31)
  and 5 more
  Carbohydrates Monosaccharides D-galactarate, D-glucarate and D-glycerate catabolism  Glycerate kinase (EC 2.7.1.31)
  Carbohydrates Monosaccharides D-galactarate, D-glucarate and D-glycerate catabolism - gjo  Glycerate kinase (EC 2.7.1.31)
  Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Glycerate kinase (EC 2.7.1.31)
  Carbohydrates Organic acids Glycerate metabolism  Glycerate kinase (EC 2.7.1.31)
  Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Glycerate kinase (EC 2.7.1.31)
• PFAM:
  no clan defined Gly_kinase; Glycerate kinase family (PF02595; HMM-score: 484.1)
  and 2 more
  NADP_Rossmann (CL0063) Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 11.6)
  no clan defined T6SS_VipA; Type VI secretion system, VipA, VC_A0107 or Hcp2 (PF05591; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.029612
  • TAT(Tat/SPI): 0.001046
  • LIPO(Sec/SPII): 0.001098
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15928010 NCBI
• RefSeq: NP_375543 NCBI
• UniProt: A0A0H3JVX2 UniProt

⊟Protein sequence

• MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIPMADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDLLEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDVNGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGADAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVVFDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENYQHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA2220 > SA2221

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications