PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2377 [new locus tag: SAUSA300_RS13150 ]
• pan locus tag^?: SAUPAN005980000
• symbol: SAUSA300_2377
• pan gene symbol^?: —
• synonym:
• product: glycerate kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2377 [new locus tag: SAUSA300_RS13150 ]
• symbol: SAUSA300_2377
• product: glycerate kinase
• replicon: chromosome
• strand: +
• coordinates: 2556402..2557544
• length: 1143
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913966 NCBI
• RefSeq: YP_495012 NCBI
• BioCyc: see SAUSA300_RS13150
• MicrobesOnline: 1293892 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
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  301
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  481
  541
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  661
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  841
  901
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  1021
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  1141
  
  ATGCAATTACAAAAAATTGTCATCGCTCCTGACTCATTTAAGGAAAGTATGACCGCACAG
  CAAGTTGGCAATATTATAAAACAGGCTTTTACTAATGTTTATGGGAATACCCTTCATTAT
  GATATCATTCCGATGGCTGATGGTGGTGAAGGTACCACAGATGCTTTAATGCATGCAACA
  GGTGCCACTAAGTATACAGTCATCGTTAATGACCCTTTAATGCGACCTATTGAAGCATGT
  TATGCACGCGCAGACGAACAACAAATTGCAATTATTGAAATGGCGGCAGCGTCAGGTTTG
  GATTTATTAGAAAAAGAGGAACGTAATCCTTTATACACATCATCATATGGTACCGGTGAA
  CTAATTAAAGATGCATTAAATCATGGTGCTAAGACCATTATTTTAGGGATTGGTGGCAGT
  GCAACAAATGATGGTGGTACAGGTATGCTAAGTGCACTAGGCGTAAAGTTTACTGATGTA
  AACGGGGACTTATTACAAATGAATGGTGCTAATCTTGCTCACATTGCACAAATCGATATA
  ACCAATCTAGATTCGCGATTAAAAGAGGTGACCTTTAAAGTGGCCTGTGATGTTTCAAAT
  CCTTTATTGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATGCA
  AAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGC
  ACAGGAAAGTCCGTTAATCAAATACCAGGTTCTGGTGCAGCTGGCGGTATGGGCGCAGCA
  TTATTAGCGTTTTGTGAGACAACTTTAACAAAAGGTATTGATGTCGTCTTTGACATTACA
  GATTTTCATCAAAGAATTAAAGATGCAGACCTCGTTATTACTGGAGAAGGACGCATGGAT
  TATCAGACCATCTTTGGTAAAACACCCGTAGGCGTTGCGTTAGCTGCAAAACAATATCAT
  ATTCCTGTCATCGCGATTTGTGGCAGTCTAGGCGAAAATTATCAACATGTTTACGATTTC
  GGTATTGATAGTGCCTATTCTATAATCTCTTCACCTAGCACTTTAGAAGATGTCCTACAA
  AATAGCGAACAAAATTTATTAAACACTGCAACTGACATTGCTCGTATTCTGAAATTACAA
  TAA
  60
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  1140
  1143
  

⊟Protein

⊟General

• locus tag: SAUSA300_2377 [new locus tag: SAUSA300_RS13150 ]
• symbol: SAUSA300_2377
• description: glycerate kinase
• length: 380
• theoretical pI: 4.76396
• theoretical MW: 40660.1
• GRAVY: -0.02

⊟Function

• reaction:
  EC 2.7.1.31?  ExPASy
  Glycerate 3-kinase ATP + D-glycerate = ADP + 3-phospho-D-glycerate
• TIGRFAM:
  Metabolism Energy metabolism Other glycerate kinase (TIGR00045; EC 2.7.1.31; HMM-score: 576.3)
• TheSEED  :
  • Glycerate kinase (EC 2.7.1.31)
  Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  Glycerate kinase (EC 2.7.1.31)
  and 5 more
  Carbohydrates Monosaccharides D-galactarate, D-glucarate and D-glycerate catabolism  Glycerate kinase (EC 2.7.1.31)
  Carbohydrates Monosaccharides D-galactarate, D-glucarate and D-glycerate catabolism - gjo  Glycerate kinase (EC 2.7.1.31)
  Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Glycerate kinase (EC 2.7.1.31)
  Carbohydrates Organic acids Glycerate metabolism  Glycerate kinase (EC 2.7.1.31)
  Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Glycerate kinase (EC 2.7.1.31)
• PFAM:
  no clan defined Gly_kinase; Glycerate kinase family (PF02595; HMM-score: 484.1)
  and 2 more
  NADP_Rossmann (CL0063) Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 11.6)
  no clan defined T6SS_VipA; Type VI secretion system, VipA, VC_A0107 or Hcp2 (PF05591; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.029612
  • TAT(Tat/SPI): 0.001046
  • LIPO(Sec/SPII): 0.001098
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161860 NCBI
• RefSeq: YP_495012 NCBI
• UniProt: A0A0H2XHY3 UniProt

⊟Protein sequence

• MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIPMADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDLLEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDVNGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGADAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVVFDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENYQHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_2377 > SAUSA300_2378

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications