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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2435 [new locus tag: SACOL_RS12780 ]
  • pan locus tag?: SAUPAN005980000
  • symbol: SACOL2435
  • pan gene symbol?: —
  • synonym:
  • product: glycerate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2435 [new locus tag: SACOL_RS12780 ]
  • symbol: SACOL2435
  • product: glycerate kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 2492841..2493983
  • length: 1143
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCAATTACAAAAAATTGTCATCGCTCCTGACTCATTTAAGGAAAGTATGACCGCACAG
    CAAGTTGGCAATATTATAAAACAGGCTTTTACTAATGTTTATGGGAATACCCTTCATTAT
    GATATCATTCCGATGGCTGATGGTGGTGAAGGTACCACAGATGCTTTAATGCATGCAACA
    GGTGCCACTAAGTATACAGTCATCGTTAATGACCCTTTAATGCGACCTATTGAAGCATGT
    TATGCACGCGCAGACGAACAACAAATTGCAATTATTGAAATGGCGGCAGCGTCAGGTTTG
    GATTTATTAGAAAAAGAGGAACGTAATCCTTTATACACATCATCATATGGTACCGGTGAA
    CTAATTAAAGATGCATTAAATCATGGTGCTAAGACCATTATTTTAGGGATTGGTGGCAGT
    GCAACAAATGATGGTGGTACAGGTATGCTAAGTGCACTAGGCGTAAAGTTTACTGATGTA
    AACGGGGACTTATTACAAATGAATGGTGCTAATCTTGCTCACATTGCACAAATCGATATA
    ACCAATCTAGATTCGCGATTAAAAGAGGTGACCTTTAAAGTGGCCTGTGATGTTTCAAAT
    CCTTTATTGGGTGAAAATGGTGCTACCTATATTTATGGTCCTCAAAAAGGCGCTGATGCA
    AAGATGATACCAAAGTTGGATTTCGCAATGTCGCATTATCATGATAAGATAAAAATGTGC
    ACAGGAAAGTCCGTTAATCAAATACCAGGTTCTGGTGCAGCTGGCGGTATGGGCGCAGCA
    TTATTAGCGTTTTGTGAGACAACTTTAACAAAAGGTATTGATGTCGTCTTTGACATTACA
    GATTTTCATCAAAGAATTAAAGATGCAGACCTCGTTATTACTGGAGAAGGACGCATGGAT
    TATCAGACCATCTTTGGTAAAACACCCGTAGGCGTTGCGTTAGCTGCAAAACAATATCAT
    ATTCCTGTCATCGCGATTTGTGGCAGTCTAGGCGAAAATTATCAACATGTTTACGATTTC
    GGTATTGATAGTGCCTATTCTATAATCTCTTCACCTAGCACTTTAGAAGATGTCCTACAA
    AATAGCGAACAAAATTTATTAAACACTGCAACTGACATTGCTCGTATTCTGAAATTACAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1143

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2435 [new locus tag: SACOL_RS12780 ]
  • symbol: SACOL2435
  • description: glycerate kinase
  • length: 380
  • theoretical pI: 4.76396
  • theoretical MW: 40660.1
  • GRAVY: -0.02

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.31?  ExPASy
    Glycerate 3-kinase ATP + D-glycerate = ADP + 3-phospho-D-glycerate
  • TIGRFAM:
    Metabolism Energy metabolism Other glycerate kinase (TIGR00045; EC 2.7.1.31; HMM-score: 576.3)
  • TheSEED  :
    • Glycerate kinase (EC 2.7.1.31)
    Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  Glycerate kinase (EC 2.7.1.31)
    and 5 more
    Carbohydrates Monosaccharides D-galactarate, D-glucarate and D-glycerate catabolism  Glycerate kinase (EC 2.7.1.31)
    Carbohydrates Monosaccharides D-galactarate, D-glucarate and D-glycerate catabolism - gjo  Glycerate kinase (EC 2.7.1.31)
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Glycerate kinase (EC 2.7.1.31)
    Carbohydrates Organic acids Glycerate metabolism  Glycerate kinase (EC 2.7.1.31)
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Glycerate kinase (EC 2.7.1.31)
  • PFAM:
    no clan defined Gly_kinase; Glycerate kinase family (PF02595; HMM-score: 484.1)
    and 2 more
    NADP_Rossmann (CL0063) Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 11.6)
    no clan defined T6SS_VipA; Type VI secretion system, VipA, VC_A0107 or Hcp2 (PF05591; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.029612
    • TAT(Tat/SPI): 0.001046
    • LIPO(Sec/SPII): 0.001098
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIPMADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDLLEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDVNGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGADAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVVFDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENYQHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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