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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA2225 [new locus tag: SA_RS12775 ]
- pan locus tag?: SAUPAN005985000
- symbol: SA2225
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA2225 [new locus tag: SA_RS12775 ]
- symbol: SA2225
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 2496959..2497765
- length: 807
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1125153 NCBI
- RefSeq: NP_375548 NCBI
- BioCyc: see SA_RS12775
- MicrobesOnline: 104574 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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781ATGAAGATAGGGACAATCGCCGATTTACATATTGATCGTCATAATAAAAAAACATCCGAA
GATTACTTAGAGGCATTGGTTGAAATAGTTAAGTATAAGAAATTGGATATATTGTTGATT
GCGGGAGATATCTCAAATCATTATCAATTGACGCACCAATTTATAACGCAATTGACAAAA
CAATTAGATATACCTGTGAAATTTGTTCCTGGAAACCATGATTTATGGGAAGTTGAAAGT
ATGACTACGCAAGACATTTGGAATAATTATAAGAGCATGTCACAGTGCTTGGTAGGAAAA
CCATTTATAGTAAATGAAGAATGGGCAATAATAGGACATACAGGTTGGTATGATTATAGC
TTTGCAGCACAACGATTTTCATTAGAACATTTACAAAAAGGAAAACATTATGGTGCGACT
TGGCAAGATAAAGAACGAATATCTTGGGGCATATCAGATCAAAATTTATCTAAAATAGCG
GCTGAACAAGTGAAGAAAGATATATTAGAAGTAGGAAATAGACGAGTGATTTTAGTCACA
CATGTTGTGACGCACCCTGATTTCATTGTTCCTATGCCACATCGTATATTCGATTTTTAT
AATGCATTTATTGGGACAAGTGATTTCAATCCTTTGTATGCGATGTTCGATATACCATAT
AGTATTATGGGCCATGTTCATTTTCGTAAAAGTGTGATGGATGATGGCAGATGTTATCTC
TGTCCGTGTCTAGGCTATCCAAGACAATGGCGTTCAGAAGATATTTACCAGGAAATAAAT
GAGACGATACAAATAATAGAAATTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA2225 [new locus tag: SA_RS12775 ]
- symbol: SA2225
- description: hypothetical protein
- length: 268
- theoretical pI: 6.44351
- theoretical MW: 31338.7
- GRAVY: -0.290672
⊟Function[edit | edit source]
- TIGRFAM: putative phosphoesterase (TIGR03729; HMM-score: 286.3)and 5 moreDNA metabolism DNA replication, recombination, and repair exonuclease SbcCD, D subunit (TIGR00619; HMM-score: 25.2)Unknown function Enzymes of unknown specificity phosphodiesterase, MJ0936 family (TIGR00040; EC 3.1.-.-; HMM-score: 22.3)TIGR04168 family protein (TIGR04168; HMM-score: 21.2)metallophosphoesterase, DNA ligase-associated (TIGR04123; HMM-score: 20.6)Unknown function Enzymes of unknown specificity putative phosphoesterase (TIGR00024; EC 3.1.-.-; HMM-score: 16.5)
- TheSEED :
- hypothetical protein
- PFAM: Calcineurin (CL0163) Metallophos; Calcineurin-like phosphoesterase (PF00149; HMM-score: 48.3)Metallophos_2; Calcineurin-like phosphoesterase superfamily domain (PF12850; HMM-score: 41.1)and 1 moreMetallophos_3; Metallophosphoesterase, calcineurin superfamily (PF14582; HMM-score: 13.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001331
- TAT(Tat/SPI): 0.000078
- LIPO(Sec/SPII): 0.000354
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDISNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPFIVNEEWAIIGHTGWYDYSFAAQRFSLEHLQKGKHYGATWQDKERISWGISDQNLSKIAAEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDIPYSIMGHVHFRKSVMDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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