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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA_RS12775 [old locus tag: SA2225 ]
  • pan locus tag?: SAUPAN005985000
  • symbol: SA_RS12775
  • pan gene symbol?: —
  • synonym:
  • product: phosphohydrolase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA_RS12775 [old locus tag: SA2225 ]
  • symbol: SA_RS12775
  • product: phosphohydrolase
  • replicon: chromosome
  • strand: -
  • coordinates: 2496959..2497765
  • length: 807
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002745 (2496959..2497765) NCBI
  • BioCyc: SA_RS12775 BioCyc
  • MicrobesOnline: see SA2225

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAGATAGGGACAATCGCCGATTTACATATTGATCGTCATAATAAAAAAACATCCGAA
    GATTACTTAGAGGCATTGGTTGAAATAGTTAAGTATAAGAAATTGGATATATTGTTGATT
    GCGGGAGATATCTCAAATCATTATCAATTGACGCACCAATTTATAACGCAATTGACAAAA
    CAATTAGATATACCTGTGAAATTTGTTCCTGGAAACCATGATTTATGGGAAGTTGAAAGT
    ATGACTACGCAAGACATTTGGAATAATTATAAGAGCATGTCACAGTGCTTGGTAGGAAAA
    CCATTTATAGTAAATGAAGAATGGGCAATAATAGGACATACAGGTTGGTATGATTATAGC
    TTTGCAGCACAACGATTTTCATTAGAACATTTACAAAAAGGAAAACATTATGGTGCGACT
    TGGCAAGATAAAGAACGAATATCTTGGGGCATATCAGATCAAAATTTATCTAAAATAGCG
    GCTGAACAAGTGAAGAAAGATATATTAGAAGTAGGAAATAGACGAGTGATTTTAGTCACA
    CATGTTGTGACGCACCCTGATTTCATTGTTCCTATGCCACATCGTATATTCGATTTTTAT
    AATGCATTTATTGGGACAAGTGATTTCAATCCTTTGTATGCGATGTTCGATATACCATAT
    AGTATTATGGGCCATGTTCATTTTCGTAAAAGTGTGATGGATGATGGCAGATGTTATCTC
    TGTCCGTGTCTAGGCTATCCAAGACAATGGCGTTCAGAAGATATTTACCAGGAAATAAAT
    GAGACGATACAAATAATAGAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    807

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA_RS12775 [old locus tag: SA2225 ]
  • symbol: SA_RS12775
  • description: phosphohydrolase
  • length: 268
  • theoretical pI: 6.44351
  • theoretical MW: 31338.7
  • GRAVY: -0.290672

⊟Function[edit | edit source]

  • TIGRFAM:
    putative phosphoesterase (TIGR03729; HMM-score: 286.3)
    and 5 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair exonuclease SbcCD, D subunit (TIGR00619; HMM-score: 25.2)
    Unknown function Enzymes of unknown specificity phosphodiesterase, MJ0936 family (TIGR00040; EC 3.1.-.-; HMM-score: 22.3)
    TIGR04168 family protein (TIGR04168; HMM-score: 21.2)
    metallophosphoesterase, DNA ligase-associated (TIGR04123; HMM-score: 20.6)
    Unknown function Enzymes of unknown specificity putative phosphoesterase (TIGR00024; EC 3.1.-.-; HMM-score: 16.5)
  • TheSEED: see SA2225
  • PFAM:
    Calcineurin (CL0163) Metallophos; Calcineurin-like phosphoesterase (PF00149; HMM-score: 48.3)
    Metallophos_2; Calcineurin-like phosphoesterase superfamily domain (PF12850; HMM-score: 41.1)
    and 1 more
    Metallophos_3; Metallophosphoesterase, calcineurin superfamily (PF14582; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001331
    • TAT(Tat/SPI): 0.000078
    • LIPO(Sec/SPII): 0.000354
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDISNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKSMSQCLVGKPFIVNEEWAIIGHTGWYDYSFAAQRFSLEHLQKGKHYGATWQDKERISWGISDQNLSKIAAEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDIPYSIMGHVHFRKSVMDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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