PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2230 [new locus tag: SA_RS12805 ]
• pan locus tag^?: SAUPAN005992000
• symbol: SA2230
• pan gene symbol^?: flp
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2230 [new locus tag: SA_RS12805 ]
• symbol: SA2230
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 2503865..2505361
• length: 1497
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125157 NCBI
• RefSeq: NP_375553 NCBI
• BioCyc: see SA_RS12805
• MicrobesOnline: 104579 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1441
  
  ATGACTACTAAAAAACTGTATTTTCTATCCATTTCTATTATCATTTTAGTCGCCATTTCA
  ATTGCTATATATATAACATTAAATAGCAATACGAAGACACGGTTAACCAATGATTCGCAA
  CAACAAATAGATAAAATTATCGAGCATGATTTACAAAAGGGACACATTCCTGGAGCATCA
  ATTTTAATAGTAAAAAATGGCAAAGTTTTTTTAAATAAAGGTTATGGTTATCAAGATGTT
  GATAAAAAAGTCAAAGCTTCTCCCACAACAAAGTATGAAATTGCTTCTAATACTAAAGCT
  TTCACAGGTCTTGCAATTTTAAAATTAGCTCAAGAAGGTCGATTAAACTTAAATGATGAC
  GTATCCAAACATGTGCCTCATTTTAAAATGAACTATAATGGTCAAAATGAAACTATTACG
  ATTAAGCAACTTTTGGCTCAAACAAGTGGTATACCTAGTGATATTACAAGCGAAGACGCC
  GTAACAAATAAAAATAATCGTTTAAATGATGTAACCCGTGCAATTATGGGTGATGAATTA
  CATCATAAGCCCGGAGAAGAATTTGAATACTCAAATATGAACTATGATTTATTAGGTTTA
  ATTATCCAAAACGTTACGAAGCAATCCTATACAAAATATATTACAAATTCATGGCTCAAG
  CCTTTGCATATGACACATACATCATTCAAACAAACCAATAACAAATCAAAACATGATGCT
  ATTGGCTACGAATTACAAGGTTCGACACCTGTCGTCTCTAAACCTGAATTTAACCTTTGG
  GATACACCATCAGCATATATGATGACATCAACTGAAGATTTGGAACATTGGATAAAATTC
  CAACTTAATCCACCTGATAAATACAAATCATTAGTTCAACAATCACATAAAAATTTATCA
  TCAACAATTGGTGAACCTAATGCCAATGCATATGCTTCGGGCTGGTTTACCAATAATGAT
  GAACATTTAGTGTTTCATTCAGGAACGCTCGATAACTTTTCATCATTTATTTTACTAAAT
  CCAAAACAAAATTATGGAATTGTTGTGCTTGCAAATCTAAATTCGGAATATGTACCCAAA
  TTAGTTGAGCATCTTAATACACAAATTGTAAATCACAAGCGATATTCGACGGTTGCGTCT
  ATACTCAATCAATATAAAGACCAATTTAATATTGTTACCGTTTTGATGACAACACTTATT
  TTATTAGCATTTATATTCTCAGCTTATCGTGCTTGGCAAATGCGCCATGGTCAAATTCTT
  TTGCGTAGATCAAAACGGATTGCTGTATTGAGTTGGCTAACTTTATGTTTATGTATCGCT
  ATAGCGCTCATATTATATGCATTACCATATCTCATTCTCGGTAGCAATAATTGGTCTTTT
  GTACTGACTTGGCTACCAATAGAAATTAAATTAGCACTAATCACAACATTAATTGCATTA
  TTCAGTACATTAATTGTAATTCTGTTATTCCTTCATACAAAGATAACGAAGACATAA
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  1497
  

⊟Protein

• 4GDN
  PDB

Protein Data Bank: 4GDN

⊟General

• locus tag: SA2230 [new locus tag: SA_RS12805 ]
• symbol: SA2230
• description: hypothetical protein
• length: 498
• theoretical pI: 9.66296
• theoretical MW: 56495.9
• GRAVY: -0.0909639

⊟Function

• TIGRFAM:
  Metabolism Energy metabolism Electron transport cytochrome c oxidase, subunit II (TIGR02866; EC 1.9.3.1; HMM-score: 8.5)
• TheSEED  :
  • beta-lactamase class C-like and penicillin binding proteins (PBPs) superfamily
  Phages, Prophages, Transposable elements, Plasmids Transposable elements Tn552  Beta-lactamase (EC 3.5.2.6)
  and 1 more
  Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Beta-lactamase  Beta-lactamase (EC 3.5.2.6)
• PFAM:
  Beta-lactamase (CL0013) Beta-lactamase; Beta-lactamase (PF00144; HMM-score: 240.4)
  and 2 more
  GPCR_A (CL0192) 7tm_3; 7 transmembrane sweet-taste receptor of 3 GCPR (PF00003; HMM-score: 16.3)
  no clan defined SIT; SHP2-interacting transmembrane adaptor protein, SIT (PF15330; HMM-score: 10.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 4
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.449986
  • TAT(Tat/SPI): 0.001717
  • LIPO(Sec/SPII): 0.091224
• predicted transmembrane helices (TMHMM): 4

⊟Accession numbers

• GI: 15928020 NCBI
• RefSeq: NP_375553 NCBI
• UniProt: Q7A3Q5 UniProt

⊟Protein sequence

• MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQIDKIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFTGLAILKLAQEGRLNLNDDVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDAVTNKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSWLKPLHMTHTSFKQTNNKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEHWIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFSSFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASILNQYKDQFNIVTVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLTLCLCIAIALILYALPYLILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications