PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2386 [new locus tag: SAUSA300_RS13205 ]
• pan locus tag^?: SAUPAN005992000
• symbol: SAUSA300_2386
• pan gene symbol^?: flp
• synonym:
• product: beta-lactamase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2386 [new locus tag: SAUSA300_RS13205 ]
• symbol: SAUSA300_2386
• product: beta-lactamase
• replicon: chromosome
• strand: +
• coordinates: 2568002..2569498
• length: 1497
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915070 NCBI
• RefSeq: YP_495021 NCBI
• BioCyc: see SAUSA300_RS13205
• MicrobesOnline: 1293901 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGACTACTAAAAAACTGTATTTTCTATCCATTTCTATTATCATTTTAGTCGCCATTTCA
  ATTGCTATATATATAACATTAAATAGCAATACGAAGACACGGTTAACCAATGATTCGCAA
  CAACAAATAGATACAATTATCGAGCATGATTTACAAAAGGGACACATTCCTGGAGCATCA
  ATTTTAATAGTAAAAAATGGCAAAGTTTTTTTAAATAAAGGTTATGGTTATCAAGATGTT
  GATAAAAAAGTCAAAGCTTCTCCCACAACAAAGTATGAAATTGCTTCTAATACGAAAGCT
  TTCACAGGTCTTGCAATTTTAAAATTAGCTCAAGAAGGTCGATTAAACTTAAATGATGCC
  GTATCCAAACATGTGCCTCATTTTAAAATGAACTATAATGGTCAAAATGAAACTATTACG
  ATTAAGCAACTTTTGGCTCAAACAAGTGGTATACCTAGTGATATTACAAGCGAAGATTCT
  GTGACAAGCAAAAATAATCGTTTAAATGATGTAACCCGTGCAATTATGGGTGATGAATTA
  CATCATAAGCCCGGAGAAGAATTTGAATACTCAAATATGAACTATGATTTATTAGGTTTA
  ATTATCCAAAACGTTACGAAGCAATCCTATACAAAATATATTACAAATTCATGGCTCAAG
  CCTTTGCATATGACACATACATCATTCAAACAAACCAATTACAAATCAAAACATGATGCT
  ATTGGCTATGAATTACAAGGTTCGACACCTGTCGTCTCTAAACCTGAATTTAACCTTTGG
  GATACACCATCAGCATATATGATGACATCAACTGAAGATTTGGAACATTGGATAAAATTC
  CAACTTAATCCACCTGATAAATACAAATCATTAGTTCAACAATCACATAAAAATTTATCT
  TCAACAATTGGTGAACCTAATGCCAATGCATATGCTTCCGGCTGGTTTACCAATAATGAT
  GAACATTTAGTGTTTCATTCAGGAACGCTAGATAACTTTTCATCATTTATTTTACTAAAT
  CCAAAACAAAATTATGGAATTGTTGTACTTGCAAATCTAAATTCGGAATATGTACCCAAA
  TTAGTTGAGCATCTTAATACACAAATTGTAAATCACAAGCGATATTCGACGGTTGCGTCT
  ATGCTCAATCAATATAAAGATCAATTTAATATTGTTACCGTTTTGATGACAACACTTATT
  TTATTAGCATTTATATTCTCAGCTTATCGTGCTTGGCAAATGCGCCATGGTCAAATTCTT
  TTGCGTAGATCAAAACGGATTGCTGTATTGAGTTGGTTATCATTATGTATATGTATCGCT
  TTAGCGCTCATATTATATGCATTACCATATCTCATTCTCGGTAGCAATAATTGGTCTTTT
  GTACTGACTTGGCTACCAATAGAAATTAAATTAGCACTAATCACAACATTAATTGCATTA
  TTCAGTACATTAATTGTAATTCTGTTATTCCTTCATACAAAGATAACGAAGACATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2386 [new locus tag: SAUSA300_RS13205 ]
• symbol: SAUSA300_2386
• description: beta-lactamase
• length: 498
• theoretical pI: 9.65027
• theoretical MW: 56466.8
• GRAVY: -0.0746988

⊟Function

• TIGRFAM:
• TheSEED  :
  • Beta-lactamase (EC 3.5.2.6)
  Virulence, Disease and Defense Resistance to antibiotics and toxic compounds Beta-lactamase  Beta-lactamase (EC 3.5.2.6)
• PFAM:
  Beta-lactamase (CL0013) Beta-lactamase; Beta-lactamase (PF00144; HMM-score: 235.1)
  and 2 more
  GPCR_A (CL0192) 7tm_3; 7 transmembrane sweet-taste receptor of 3 GCPR (PF00003; HMM-score: 17.1)
  no clan defined SIT; SHP2-interacting transmembrane adaptor protein, SIT (PF15330; HMM-score: 10.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 4
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide SP(Sec/SPI) length 33 aa
  • SP(Sec/SPI): 0.46193
  • TAT(Tat/SPI): 0.001668
  • LIPO(Sec/SPII): 0.089464
  • Cleavage Site: CS pos: 33-34. TKT-RL. Pr: 0.0532
• predicted transmembrane helices (TMHMM): 4

⊟Accession numbers

• GI: 87160635 NCBI
• RefSeq: YP_495021 NCBI
• UniProt: A0A0H2XF03 UniProt

⊟Protein sequence

• MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQIDTIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFTGLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDSVTSKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSWLKPLHMTHTSFKQTNYKSKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEHWIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFSSFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKDQFNIVTVLMTTLILLAFIFSAYRAWQMRHGQILLRRSKRIAVLSWLSLCICIALALILYALPYLILGSNNWSFVLTWLPIEIKLALITTLIALFSTLIVILLFLHTKITKT

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications