PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2277
• pan locus tag^?: SAUPAN006085000
• symbol: SA2277
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2277
• symbol: SA2277
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2553138..2554469
• length: 1332
• essential: no DEG

⊟Accession numbers

• Gene ID: 1125205 NCBI
• RefSeq: NP_375601 NCBI
• BioCyc:
• MicrobesOnline: 104627 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATGAGTAGATTACTAAATGATTTCAATCAATCTTTAAAAAAAGGTTTTATAGACAAAGAT
  ATTTCTCATAAAGGGAATTATACACCGAAGCTTTTAGTAAATAATAAAAATGAAAAAGTC
  CTTTCAACAATTATAGATGAACTTCAAAAATGTGAAACGTTCTATTTTTCTGTAGCTTTT
  ATAACAGAAAGCGGATTAGCCAGCTTAAAGGCTCAACTATTAGATTTAAGCAATAAGGGT
  GTTAAAGGGAAAATATTAACATCTAATTACTTAGGATTTAATAGTCCTAAAATGTATGGA
  GAATTACTTAAATTAAAAAATGTAGAGGTAAGATTAACTGATATTGCTGGATTCCATGCC
  AAAGGATATATTTTTGAACATAAGGATTATAGTTCTATGGTAATAGGAAGCTCTAATTTA
  ACATCTAATGCGTTAAAGGTTAATTATGAGCATAATGTTTTACTGTCTACTATGAAAAAT
  GGTGATTTAGTTGATAGTGTAAAAAATGAATTTGAATTGTTATGGCAAAAGAGTACCCCA
  CTGACTGAGCAATGGATTAATTCATATAAAGAATCATTTGAGTACCGTTCCTTAGAAAAA
  TTAGCGGAGGTTGAACAAACTCAAATGCTGCTAGCTGATAAGGTGAAAAAATCAGTGGAA
  ATTGTACCCAATTTAATGCAAGCAGAAGCATTAAGGTCATTAAAAGCTATTAGGGATAAA
  ACCAAAGATAAGGCGTTAATAATATCTGCAACTGGTACAGGTAAAACGATTTTATGTGCA
  TTAGATGTTAGAGAAGTAAACCCTAATAAATTTTTATTTATTGTTCATAATGAGGGGATT
  TTAAATAGAGCTAAGGAAGAATTTAAAAAAGTATTACCTATAAAAAATGATAGTGATTTT
  GGATTGTTGACAGGAAAACATAGAGATGTCGATGCCAAATATTTATTTGCAACCATTCAA
  ACATTATCTAGAGATGATAATTTTAAGCAATTTGATGAAAATGAATTTGATTATATTGTT
  TTTGATGAAGCACATCGTTCTGCTGCATCTACTTATCAACGAGTATTTAACTACTTCAAA
  CCTAAGTTCATGCTGGGAATGACTGCTACACCAGAAAGATCAGATGAATTAAGTATATTT
  GAACTGTTTGATTATAATATTGCATATGAAATAAGGTTACAAGCAGCGTTAGAAAGTGAT
  ATTTTATGTCCATTTCATTATTTTGGTGTGACTGATTATGTACATCAAGGTATTAAAGAA
  GATGATGTAACTAAACTGAGATATTTAACTTCTGATGAAAGAGTTAATTATATTATTCAA
  AAGACAGATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1332
  

⊟Protein

⊟General

• locus tag: SA2277
• symbol: SA2277
• description: hypothetical protein
• length: 443
• theoretical pI: 6.80364
• theoretical MW: 50847.7
• GRAVY: -0.361851

⊟Function

• TIGRFAM:
  DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 29.7)
  and 1 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 21.2)
• TheSEED  :
  • DNA/RNA helicase of DEAD/DEAH box family
• PFAM:
  P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 123.2)
  and 4 more
  PLD (CL0479) PLDc_2; PLD-like domain (PF13091; HMM-score: 57.8)
  P-loop_NTPase (CL0023) DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 27.5)
  DUF2075; Uncharacterized conserved protein (DUF2075) (PF09848; HMM-score: 22)
  SNF2_N; SNF2 family N-terminal domain (PF00176; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005967
  • TAT(Tat/SPI): 0.000178
  • LIPO(Sec/SPII): 0.000377
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15928068 NCBI
• RefSeq: NP_375601 NCBI
• UniProt: A0A0H3JMV5 UniProt

⊟Protein sequence

• MSRLLNDFNQSLKKGFIDKDISHKGNYTPKLLVNNKNEKVLSTIIDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGELLKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKNGDLVDSVKNEFELLWQKSTPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKSVEIVPNLMQAEALRSLKAIRDKTKDKALIISATGTGKTILCALDVREVNPNKFLFIVHNEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDENEFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIRLQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTD

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SA2276 < SA2277 < SA2278

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications