From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2287 [new locus tag: SA_RS13115 ]
  • pan locus tag?: SAUPAN006110000
  • symbol: sarH2
  • pan gene symbol?: sarU
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SA2287 [new locus tag: SA_RS13115 ]
  • symbol: sarH2
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2564802..2565545
  • length: 744
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    GTGGACTATCAAACTTTCGAAAAGGTCAATAAATTCATAAATGTAGAAGCGTACATATTT
    TTTCTCACTCAAGAGTTAAAGCAACAATATAAATTATCATTAAAAGAATTGTTGATATTA
    GCATATTTTTATTACAAAAATGAACACAGTATTTCACTAAAAGAAATCATTGGTGACATA
    CTTTACAAACAATCTGATGTTGTAAAGAACATTAAGTCACTATCTAAAAAAGGATTTATA
    AATAAGTCTAGAAACGAAGCAGATGAACGCCGTATTTTTGTTTCAGTTACTCCAATACAA
    CGTAAAAAGATTGCTTGTGTTATTAATGAGTTAGATAAAATAATTAAAGGATTTAATAAG
    GAAAGAGACTACATAAAATATCAATGGGCTCCAAAATATAGCAAAGAATTTTTTATACTT
    TTTATGAACATTATGTACTCAAAAGATTTTTTAAAATATCGATTTAATTTAACATTTCTT
    GATTTATCTATCTTATATGTAATATCATCTCGAAAAAATGAGATACTAAATTTAAAAGAT
    TTGTTTGAAAGTATTAGATTTATGTATCCTCAAATTGTTAGGTCAGTTAATAGATTAAAT
    AATAAAGGTATGCTAATCAAAGAACGATCCCTTGCAGATGAAAGGATTGTGTTAATCAAA
    ATAAATAAAATACAATATAACACTATTAAAAGCATATTCACAGATACTTCCAAGATTCTC
    AAACCAAGAAAATTTTTCTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    744

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SA2287 [new locus tag: SA_RS13115 ]
  • symbol: SarH2
  • description: hypothetical protein
  • length: 247
  • theoretical pI: 10.3367
  • theoretical MW: 29792.9
  • GRAVY: -0.245344

Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions staphylococcal accessory regulator family (TIGR01889; HMM-score: 210.6)
    and 1 more
    homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 35.4)
  • TheSEED  :
    • Transcriptional regulator SarU (Staphylococcal accessory regulator U)
  • PFAM:
    HTH (CL0123) MarR; MarR family (PF01047; HMM-score: 36.3)
    HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 34.9)
    MarR_2; MarR family (PF12802; HMM-score: 29.8)
    and 4 more
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 19.3)
    Rrf2; Transcriptional regulator (PF02082; HMM-score: 14.2)
    HTH_Tnp_4; Helix-turn-helix of DDE superfamily endonuclease (PF13613; HMM-score: 14.1)
    OB (CL0021) CSD; 'Cold-shock' DNA-binding domain (PF00313; HMM-score: 12.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002072
    • TAT(Tat/SPI): 0.000099
    • LIPO(Sec/SPII): 0.000363
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFYYKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERRIFVSVTPIQRKKIACVINELDKIIKGFNKERDYIKYQWAPKYSKEFFILFMNIMYSKDFLKYRFNLTFLDLSILYVISSRKNEILNLKDLFESIRFMYPQIVRSVNRLNNKGMLIKERSLADERIVLIKINKIQYNTIKSIFTDTSKILKPRKFFF

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]