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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2306 [new locus tag: SA_RS13225 ]
  • pan locus tag?: SAUPAN006162000
  • symbol: SA2306
  • pan gene symbol?: mhqD
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2306 [new locus tag: SA_RS13225 ]
  • symbol: SA2306
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2592349..2592942
  • length: 594
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGAACATATTTTTCGTGAAGGACAAAAAGGTGCACCAACGCTAATATTGTTGCATGGT
    ACAGGTGGCGATGAATTCGATTTATTGCCATTAGGCGAAGCATTGAATGAAAATTATCAC
    TTGTTAAGTATTAGAGGACAAGTTTCAGAAAATGGGATGAACCGGTATTTCAAACGTCTT
    GGTGAAGGTGTTTATGATGAAGAAGATTTGGAATTTCGTGGACAAGAATTGTTAGCGTTC
    ATTAAAGAAGCTGCTGAACGTTATGAATTTGATATTGAAAAAGCAGTACTTGTTGGATTT
    TCAAATGGATCAAATATAGCGATTAACTTAATGTTGCGTTCGGAAGCACCATTTAAAAAA
    GCATTGTTATATGCACCGTTATACCCAGTTGAAGTAACGTCAACAAAGGATTTATCAGAT
    GTAAGTGTGTTGCTTTCTATGGGGAAACATGATCCAATTGTGCCATTAGCTGCAAGTGAA
    CAAGTCATTAACTTGTTTAAGACACGTGGGGCAGTAGTCGAAGAAGTTTGGGTGAATGGT
    CATGAAATTACAGAAACTGGATTAACGGCTGGTCAACAAATACTTGGGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    594

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2306 [new locus tag: SA_RS13225 ]
  • symbol: SA2306
  • description: hypothetical protein
  • length: 197
  • theoretical pI: 4.5712
  • theoretical MW: 21819.7
  • GRAVY: -0.124365

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 22.2)
    and 2 more
    pyrimidine utilization protein D (TIGR03611; HMM-score: 17)
    Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 13.6)
  • TheSEED  :
    • Carboxylesterase (EC 3.1.1.1)
  • PFAM:
    AB_hydrolase (CL0028) Abhydrolase_2; Phospholipase/Carboxylesterase (PF02230; HMM-score: 41.5)
    and 11 more
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 27.2)
    DLH; Dienelactone hydrolase family (PF01738; HMM-score: 23.7)
    Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 20.8)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 20.1)
    Esterase; Putative esterase (PF00756; HMM-score: 19.1)
    Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 17.8)
    DUF915; Alpha/beta hydrolase of unknown function (DUF915) (PF06028; HMM-score: 15.5)
    FSH1; Serine hydrolase (FSH1) (PF03959; HMM-score: 15.1)
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 13.3)
    Abhydrolase_4; TAP-like protein (PF08386; HMM-score: 13.3)
    DUF676; Putative serine esterase (DUF676) (PF05057; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007145
    • TAT(Tat/SPI): 0.000484
    • LIPO(Sec/SPII): 0.002035
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEHIFREGQKGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSIRGQVSENGMNRYFKRLGEGVYDEEDLEFRGQELLAFIKEAAERYEFDIEKAVLVGFSNGSNIAINLMLRSEAPFKKALLYAPLYPVEVTSTKDLSDVSVLLSMGKHDPIVPLAASEQVINLFKTRGAVVEEVWVNGHEITETGLTAGQQILGK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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