From AureoWiki
Jump to navigation Jump to search

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA2421 [new locus tag: SA_RS13885 ]
  • pan locus tag?: SAUPAN006354000
  • symbol: SA2421
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA2421 [new locus tag: SA_RS13885 ]
  • symbol: SA2421
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2716718..2717191
  • length: 474
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    TTGAAAGGAGTCATCATCATGTCGACCAATAAAAACGATTATGAGCATATGTTGTTTTAT
    TTTGCATATAAAACGTTTATTACTACCGCTGATGAAATTATAGAGAAGTATGGTATGAGC
    CGTCAGCATCATCGTTTTTTGTTTTTTATCAATAAATTACCTGGTATTACTATTAAATCA
    TTACTAGAAATATTAGAAATTTCTAAACAAGGATCACATGCAACACTTCAAAAATTAAAA
    GAGCAAGGTCTCATTATTGAAAAAGTTTTAGAGACTGATCGACGTGTCAAAAAATTATAT
    TCGACGGATAAAGGCGATCAACTCATTGCTGAATTGAACAAGGCGCAAGATGAATTATTG
    CAAAATATATATCAACAAGTCGGTTCGGATTGGTATGATGTGATGGAAGCATTGGCTAAA
    GGGCGACCTGGCTTTGATTTTATTAAGCATTTGAAAGATGAAAAAGAAAGCTAG
    60
    120
    180
    240
    300
    360
    420
    474

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA2421 [new locus tag: SA_RS13885 ]
  • symbol: SA2421
  • description: hypothetical protein
  • length: 157
  • theoretical pI: 7.85755
  • theoretical MW: 18334
  • GRAVY: -0.442038

⊟Function[edit | edit source]

  • TIGRFAM:
    mobile rSAM pair MarR family regulator (TIGR04472; HMM-score: 29.7)
    homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 24.6)
    and 1 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair S4 domain protein YaaA (TIGR02988; HMM-score: 13.3)
  • TheSEED  :
    • Transcriptional regulator, MarR family
  • PFAM:
    HTH (CL0123) MarR_2; MarR family (PF12802; HMM-score: 50.5)
    and 11 more
    HTH_11; HTH domain (PF08279; HMM-score: 23.8)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 18.2)
    HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 17.1)
    MarR; MarR family (PF01047; HMM-score: 16.9)
    no clan defined PhaP_Bmeg; Polyhydroxyalkanoic acid inclusion protein (PhaP_Bmeg) (PF09602; HMM-score: 16.9)
    HTH (CL0123) HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 16.5)
    F-93_WHD; F-93, winged-helix domain (PF22194; HMM-score: 13.8)
    ANL (CL0378) GH3_N_vert; Vertebrate GH3, N-terminal domain (PF25146; HMM-score: 13.5)
    HTH (CL0123) HxlR; HxlR-like helix-turn-helix (PF01638; HMM-score: 13.4)
    MCM_C; MCM protein C-terminal winged helix-turn-helix domain (PF21100; HMM-score: 13.4)
    HTH_67; Helix-turn-helix family (PF21863; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8717
    • Cytoplasmic Membrane Score: 0.1171
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.011
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002253
    • TAT(Tat/SPI): 0.000067
    • LIPO(Sec/SPII): 0.000434
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKGVIIMSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFINKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQLIAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRPGFDFIKHLKDEKES

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://aureowiki.med.uni-greifswald.de/index.php?title=SA2421&oldid=98771"