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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0007 [new locus tag: SACOL_RS00055 ]
  • pan locus tag?: SAUPAN000013000
  • symbol: SACOL0007
  • pan gene symbol?: nnrD
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0007 [new locus tag: SACOL_RS00055 ]
  • symbol: SACOL0007
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 9782..10483
  • length: 702
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGTTAGCGGCTCGTGCATGTGTATTTAGCGGTAGTGGTTTAATCACTGTAGCTACACAT
    CCAACAAATCATTCAGCATTACATTCTCGTTGCCCAGAAGCGATGGTTATTGATATTAAT
    GATACGAAAATGTTGACGAAAATGATTGAAATGACTGACAGTATACTAATTGGTCCAGGT
    CTTGGCGTTGATTTCAAAGGAAATAATGCCATTACATTCCTACTACAAAATATACAACCG
    CATCAAAATTTAATCGTAGACGGCGATGCGATTACAATCTTTAGTAAACTGAAACCGCAA
    TTACCTACATGTCGTGTGATCTTTACACCACACCTCAAAGAATGGGAACGATTAAGTGGT
    ATTCCTATTGAGGAACAGACATATGAGCGTAATCGTGAAGCAGTTGATCGTTTAGGTGCA
    ACTGTTGTACTTAAAAAACATGGTACTGAAATTTTCTTTAAAGATGAAGACTTTAAATTG
    ACAATCGGTAGCCCAGCAATGGCGACTGGTGGTATGGGCGATACACTTGCTGGTATGATT
    ACAAGCTTTGTCGGTCAATTTGATAACTTAAAAGAAGCGGTTATGAGTGCCACATATACA
    CATAGTTTTATTGGCGAAAACCTTGCAAAAGATATGTATGTGGTGCCACCATCAAGACTT
    ATCAATGAAATACCTTACGCAATGAAACAATTAGAAAGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    702

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0007 [new locus tag: SACOL_RS00055 ]
  • symbol: SACOL0007
  • description: hypothetical protein
  • length: 233
  • theoretical pI: 6.1537
  • theoretical MW: 25655.6
  • GRAVY: -0.00515021

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.136?  ExPASy
    ADP-dependent NAD(P)H-hydrate dehydratase ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide = AMP + phosphate + NADH ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine-dinucleotide phosphate = AMP + phosphate + NADPH
  • TIGRFAM:
    Unknown function General YjeF family C-terminal domain (TIGR00196; HMM-score: 137.8)
    and 2 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 28.4)
    Metabolism Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 14)
  • TheSEED  :
    • ADP-dependent (S)-NAD(P)H-hydrate dehydratase (EC 4.2.1.136)
  • PFAM:
    Ribokinase (CL0118) Carb_kinase; Carbohydrate kinase (PF01256; HMM-score: 175)
    and 1 more
    Phos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 30.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.67
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.347222
    • TAT(Tat/SPI): 0.031707
    • LIPO(Sec/SPII): 0.038771
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLAARACVFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDFKGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPIEEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMITSFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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