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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0031 [new locus tag: SACOL_RS00175 ]
  • pan locus tag?: SAUPAN000099000
  • symbol: SACOL0031
  • pan gene symbol?: ugpQ
  • synonym:
  • product: glycerophosphoryl diester phosphodiesterase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0031 [new locus tag: SACOL_RS00175 ]
  • symbol: SACOL0031
  • product: glycerophosphoryl diester phosphodiesterase
  • replicon: chromosome
  • strand: +
  • coordinates: 38329..39072
  • length: 744
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGCTAACTGTATATGGACATAGAGGATTACCTAGTAAAGCTCCGGAAAATACAATTGCA
    TCATTTAAAGCTGCTTCAGAAGTAGAAGGTATAAACTGGTTGGAGTTAGATGTTGCAATT
    ACAAAAGATGAACAACTGATTATCATTCATGATGATTATTTAGAACGGACTACAAATATG
    TCCGGGGAAATAACTGAATTGAATTATGATGAAATTAAAGATGCTTCTGCAGGATCTTGG
    TTTGGTGAAAAATTCAAAGATGAACATTTGCCAACTTTCGATGATGTAGTAAAAATAGCA
    AATGAATATAATATGAATTTAAATGTAGAATTAAAAGGTATTACTGGACCGAATGGACTA
    GCACTTTCTAAAAGTATGGTTAAGCAAGTGGAAGAACAATTAACAAACTTAAATCAGAAT
    CAAGAAGTGCTCATTTCAAGCTTTAATGTTGTGCTTGTTAAACTTGCAGAAGAAATCATG
    CCACAATATAACAGAGCAGTTATATTCCATACAACTTCGTTTCGTGAAGACTGGAGAACA
    CTTTTAGATTACTGTAATGCTAAAATAGTAAACACTGAAGATGCCAAACTTACTAAAGCA
    AAAGTAAAAATGGTAAAAGAAGCGGGTTATGAATTGAACGTATGGACTGTAAACAAACCA
    GCACGTGCAAACCAACTTGCTAATTGGGGAGTTGATGGTATCTTTACAGACAATGCAGAT
    AAAATGGTGCATTTGTCTCAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    744

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0031 [new locus tag: SACOL_RS00175 ]
  • symbol: SACOL0031
  • description: glycerophosphoryl diester phosphodiesterase
  • length: 247
  • theoretical pI: 4.65848
  • theoretical MW: 27899.4
  • GRAVY: -0.346559

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Methanogenesis tetrahydromethanopterin S-methyltransferase, subunit B (TIGR04166; EC 2.1.1.86; HMM-score: 12.4)
  • TheSEED  :
    • Glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46)
    Carbohydrates Sugar alcohols Glycerol and Glycerol-3-phosphate Uptake and Utilization  Glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46)
  • PFAM:
    TIM_barrel (CL0036) GDPD; Glycerophosphoryl diester phosphodiesterase family (PF03009; HMM-score: 172.2)
    and 4 more
    GDPD_2; Glycerophosphoryl diester phosphodiesterase family (PF13653; HMM-score: 21.3)
    RNase_H (CL0219) TNP-like_RNaseH_N; TNP-like, RNase H N-terminal domain (PF21787; HMM-score: 13.6)
    E-set (CL0159) Ig_mannosidase; Ig-fold domain (PF17753; HMM-score: 12.8)
    no clan defined Snapin_Pallidin; Snapin/Pallidin (PF14712; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.93
    • Cytoplasmic Membrane Score: 0.0307
    • Cell wall & surface Score: 0.0055
    • Extracellular Score: 0.0338
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003291
    • TAT(Tat/SPI): 0.000269
    • LIPO(Sec/SPII): 0.000434
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDEQLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANEYNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIMPQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVNKPARANQLANWGVDGIFTDNADKMVHLSQ

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell: 46 [3]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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