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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40
NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL0036 [new locus tag: SACOL_RS00200 ]
- pan locus tag?: SAUPAN000107000
- symbol: SACOL0036
- pan gene symbol?: —
- synonym:
- product: IS1272 transposase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL0036 [new locus tag: SACOL_RS00200 ]
- symbol: SACOL0036
- product: IS1272 transposase
- replicon: chromosome
- strand: -
- coordinates: 42958..44481
- length: 1524
- essential: unknown
⊟Accession numbers[edit | edit source]
- Gene ID: 3236766 NCBI
- RefSeq: YP_184947 NCBI
- BioCyc: see SACOL_RS00200
- MicrobesOnline: 911520 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1501ATGATGTTAAAAGTGATTCTATATGCCTACACACAATCTGTATTCTCAGGTCGTAAAATA
GAAAAAATGCTTAATGATAGCATCCGAATGATGTGGCTATCACAAAATCAAAAACCTTCT
TATAAAACAATTAATCAATTTAGAGTAAATCCAAAAGTAGATGCTTTATTAGAATCTTTA
TTTATTCAATTTTACAGTCAGTGTGTAAAACAAAATCTTATAGATGATAAAGCTATTTTT
ATTGATGGTACAAAAATTGAAGCAAATGCCAATCGATATACATTTGTATGGAAAAAGAGT
ATTCAAAACCATGAATCAAAGATGAATGAGGATTCTAAAGCCCTCTACCATGAATTGGTA
ACCAATAAAATCATACCGGAAATTAAAGAAGATCATGATAATGAATTAACAAAAGAAGAA
ATAGATTTGATTGGTAGTCACTTAGATAAAGAAATCGAAGATTTAAACCAACATATCAAC
AATGAAAAATGTACTAAAACAAGAAAACAAATACGTCTCAAAAGAACTAAAATCAAAAAA
TACAAAAAGCAAATCAATGATTATTTTGAGCGAAAGTATCGATACGAATTTCAAAAATCT
ATTTTAAAGGATAGAAATAGTTATTCTAAGACAGATTATGATGCGACATTTATGAGAATG
AAAGAAGATCACATGAAAAATGGACAACTTAAGCCAGGGTATAATTTACAAATAGCAACA
AATTCCCAATTTGTTTTATCTTATAATGTGTATCAAAATCCAACGGATACTAGAACGATG
ATTCCATTTTTAAATTCAATTCAAGAGACCTACGGTCATTTACCTGAATATATTGTAGCT
GATGCAGGTTATGGTAGTGAATCAAATTATAAGGCAATTATAGATGACTTTAATCGAACG
CCACTCATAACATATGGAATGTTTATAAAAGATAAAACTAAAAAATATAAAAGTGACATC
TTTAATACTCAAAATTGGAACTATGACGAAATTAATGACGAATTCATTTGTCCGAATAAT
AAACGGCTAGGTTTTAAAAGATATGCCTATCGTCATGATAAGTATGGTTATAAGCGAGAC
TTCAAATTATATGAATGTGATGATTGTTCAGAATGTCCTCTGAAAAATCAATGTATGAAC
TTCAATTCAAAAACAAACAAAAAAATAATGAAGAATTATAACTGGGAATATTTTAAATCC
CAAATTAACAAAAAGCTTTCAGAACCAGAAACAAAAAATATCTACAGTCAAAGAAAAATT
GATGTGGAACCTGTTTTTGGATTTATGAAGGCTATTTTGGGTTTCACTCGGATGTCTGTC
CGAGGACTCAATAAAGTCAAAAGAGAACTTGGTTTTGTATTAATGGCACTTAATATAAGA
AAAGTAGTAGCTCAACGAGCTGAAAATAATCAAAAAATTTATAAAAAAGACAATTTCTAT
ATTATTTCAATAGAAATTGTCTTTTTTCACTTATCCAAGAACTTTATGTCCCGGACTCAT
TATGATTTATTTTTTTATTGCTGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL0036 [new locus tag: SACOL_RS00200 ]
- symbol: SACOL0036
- description: IS1272 transposase
- length: 507
- theoretical pI: 9.74588
- theoretical MW: 60733.3
- GRAVY: -0.768245
⊟Function[edit | edit source]
- TIGRFAM:
- TheSEED :
- putative transposase
- PFAM: RNase_H (CL0219) DDE_Tnp_1; Transposase DDE domain (PF01609; HMM-score: 129.9)and 4 moreDDE_Tnp_1_6; Transposase DDE domain (PF13751; HMM-score: 93.7)no clan defined DUF772; Transposase domain (DUF772) (PF05598; HMM-score: 40.9)UPF0179; Uncharacterised protein family (UPF0179) (PF03684; HMM-score: 10.8)Viral_ssRNA_CP (CL0055) Circo_capsid; Circovirus capsid protein (PF02443; HMM-score: 8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 0
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.015934
- TAT(Tat/SPI): 0.000225
- LIPO(Sec/SPII): 0.005437
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MMLKVILYAYTQSVFSGRKIEKMLNDSIRMMWLSQNQKPSYKTINQFRVNPKVDALLESLFIQFYSQCVKQNLIDDKAIFIDGTKIEANANRYTFVWKKSIQNHESKMNEDSKALYHELVTNKIIPEIKEDHDNELTKEEIDLIGSHLDKEIEDLNQHINNEKCTKTRKQIRLKRTKIKKYKKQINDYFERKYRYEFQKSILKDRNSYSKTDYDATFMRMKEDHMKNGQLKPGYNLQIATNSQFVLSYNVYQNPTDTRTMIPFLNSIQETYGHLPEYIVADAGYGSESNYKAIIDDFNRTPLITYGMFIKDKTKKYKSDIFNTQNWNYDEINDEFICPNNKRLGFKRYAYRHDKYGYKRDFKLYECDDCSECPLKNQCMNFNSKTNKKIMKNYNWEYFKSQINKKLSEPETKNIYSQRKIDVEPVFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAENNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization: Cytoplasmic [1]
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: no data available
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)