⊟Summary[edit | edit source]
- pan ID?: SAUPAN000107000
- symbol?: —
- synonym:
- description?: IS5/IS1182 family transposase
- IS5/IS1182 family transposase
- IS1272, transposase
- IS1272 transposase
- ISSau3 transposase
- putative transposase
- transposase DDE domain protein
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 125375..138638
- synteny block?: BlockID0000850
- occurrence?: in 29% of 34 strains
tnpIS1272 : IS1272mec transposase [1] [1]
Staphylococci containing the Staphylococcal Chromosomal Cassette-methicillin resistance elements (SCCmec) will often contain an IS1272mec transposase truncating the MecR1 signal transducer protein. This IS5/IS1182/ISSau3 family transposase then forms the boundary between "class B" mec elements and the J2 region, particularly in SCCmec type I, IV and VI strains and plays a significant role in SCCmec subtyping. With standardization of SCCmec nomenclature, IS1272mec has become the established name for this transposase in SCCmec elements, although alternative nomenclature including IS1181, IS1182, ISSau3 and ISSep1 and IS663 have been used. Formally, IS1182mec is the nomenclature given if the entire coding sequence is in frame while IS1272mec requires recoding by frameshift, although sequencing and assembly errors can make such distinctions challenging. Outside of SCCmec elements where these nuances are important for subtyping, the more generic "ISSau3" nomenclature is typically preferred.
⊟Orthologs[edit | edit source]
⊟Genome Viewer[edit | edit source]
| COL | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MMLKVILYAYTQSVFSGRKIEKMLNDSIRMMWLSQNQKPSYKTINQFRVNPKVDALLESL
USA300_FPR3757 ----MILYAYTQSVFSGRKIEKMLNDSIRMMWLSQNQKPSYKTINQFRVNPKVDALLESL
:*******************************************************
COL FIQFYSQCVKQNLIDDKAIFIDGTKIEANANRYTFVWKKSIQNHESKMNEDSKALYHELV
USA300_FPR3757 FIQFYSQCVKQNLIDDKAIFIDGTKIEANANRYTFVWKKSIQNHESKMNEDSKALYHELV
************************************************************
COL TNKIIPEIKEDHDNELTKEEIDLIGSHLDKEIEDLNQHINNEKCTKTRKQIRLKRTKIKK
USA300_FPR3757 TNKIIPEIKEDHDNELTKEEIDLIGSHLDKEIEDLNQHINNEKCTKTRKQIRLKRTKIKK
************************************************************
COL YKKQINDYFERKYRYEFQKSILKDRNSYSKTDYDATFMRMKEDHMKNGQLKPGYNLQIAT
USA300_FPR3757 YKKQINDYFERKYRYEFQKSILKDRNSYSKTDYDATFMRMKEDHMKNGQLKPGYNLQIAT
************************************************************
COL NSQFVLSYNVYQNPTDTRTMIPFLNSIQETYGHLPEYIVADAGYGSESNYKAIIDDFNRT
USA300_FPR3757 NSQFVLSYNVYQNPTDTRTMIPFLNSIQETYGHLPEYIVADAGYGSESNYKAIIDDFNRT
************************************************************
COL PLITYGMFIKDKTKKYKSDIFNTQNWNYDEINDEFICPNNKRLGFKRYAYRHDKYGYKRD
USA300_FPR3757 PLITYGMFIKDKTKKYKSDIFNTQNWNYDEINDEFICPNNKRLGFKRYAYRHDKYGYKRD
************************************************************
COL FKLYECDDCSECPLKNQCMNFNSKTNKKIMKNYNWEYFKSQINKKLSEPETKNIYSQRKI
USA300_FPR3757 FKLYECDDCSECPLKNQCMNFNSKTNKKIMKNYNWEYFKSQINKKLSEPETKNIYSQRKI
************************************************************
COL DVEPVFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAENNQKIYKKDNFY
USA300_FPR3757 DVEPVFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAENNQKIYKKDNFY
************************************************************
COL IISIEIVFFHLSKNFMSRTHYDLFFYC
USA300_FPR3757 IISIEIVFFHLSKNFMSRTHYDLFFYC
***************************
- ↑ G L Archer, J A Thanassi, D M Niemeyer, M J Pucci
Characterization of IS1272, an insertion sequence-like element from Staphylococcus haemolyticus.
Antimicrob Agents Chemother: 1996, 40(4);924-9
[PubMed:8849253] [WorldCat.org] [DOI] (P p)N Kobayashi, S Urasawa, N Uehara, N Watanabe
Distribution of insertion sequence-like element IS1272 and its position relative to methicillin resistance genes in clinically important Staphylococci.
Antimicrob Agents Chemother: 1999, 43(11);2780-2
[PubMed:10543763] [WorldCat.org] [DOI] (P p)Liang Chen, José R Mediavilla, Duarte C Oliveira, Barbara M Willey, Herminia de Lencastre, Barry N Kreiswirth
Multiplex real-time PCR for rapid Staphylococcal cassette chromosome mec typing.
J Clin Microbiol: 2009, 47(11);3692-706
[PubMed:19726600] [WorldCat.org] [DOI] (I p)