PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0034 [new locus tag: SAUSA300_RS00180 ]
• pan locus tag^?: SAUPAN000107000
• symbol: SAUSA300_0034
• pan gene symbol^?: —
• synonym:
• product: IS1272, transposase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0034 [new locus tag: SAUSA300_RS00180 ]
• symbol: SAUSA300_0034
• product: IS1272, transposase
• replicon: chromosome
• strand: -
• coordinates: 42442..43953
• length: 1512
• essential: unknown

⊟Accession numbers

• Gene ID: 3913755 NCBI
• RefSeq: YP_492755 NCBI
• BioCyc: see SAUSA300_RS00180
• MicrobesOnline: 1291550 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  GTGATTCTATATGCCTACACACAATCTGTATTCTCAGGTCGTAAAATAGAAAAAATGCTT
  AATGATAGCATCCGAATGATGTGGCTATCACAAAATCAAAAACCTTCTTATAAAACAATT
  AATCAATTTAGAGTAAATCCAAAAGTAGATGCTTTATTAGAATCTTTATTTATTCAATTT
  TACAGTCAGTGTGTAAAACAAAATCTTATAGATGATAAAGCTATTTTTATTGATGGTACA
  AAAATTGAAGCAAATGCCAATCGATATACATTTGTATGGAAAAAGAGTATTCAAAACCAT
  GAATCAAAGATGAATGAGGATTCTAAAGCCCTCTACCATGAATTGGTAACCAATAAAATC
  ATACCGGAAATTAAAGAAGATCATGATAATGAATTAACAAAAGAAGAAATAGATTTGATT
  GGTAGTCACTTAGATAAAGAAATCGAAGATTTAAACCAACATATCAACAATGAAAAATGT
  ACTAAAACAAGAAAACAAATACGTCTCAAAAGAACTAAAATCAAAAAATACAAAAAGCAA
  ATCAATGATTATTTTGAGCGAAAGTATCGATACGAATTTCAAAAATCTATTTTAAAGGAT
  AGAAATAGTTATTCTAAGACAGATTATGATGCGACATTTATGAGAATGAAAGAAGATCAC
  ATGAAAAATGGACAACTTAAGCCAGGGTATAATTTACAAATAGCAACAAATTCCCAATTT
  GTTTTATCTTATAATGTGTATCAAAATCCAACGGATACTAGAACGATGATTCCATTTTTA
  AATTCAATTCAAGAGACCTACGGTCATTTACCTGAATATATTGTAGCTGATGCAGGTTAT
  GGTAGTGAATCAAATTATAAGGCAATTATAGATGACTTTAATCGAACGCCACTCATAACA
  TATGGAATGTTTATAAAAGATAAAACTAAAAAATATAAAAGTGACATCTTTAATACTCAA
  AATTGGAACTATGACGAAATTAATGACGAATTCATTTGTCCGAATAATAAACGGCTAGGT
  TTTAAAAGATATGCCTATCGTCATGATAAGTATGGTTATAAGCGAGACTTCAAATTATAT
  GAATGTGATGATTGTTCAGAATGTCCTCTGAAAAATCAATGTATGAACTTCAATTCAAAA
  ACAAACAAAAAAATAATGAAGAATTATAACTGGGAATATTTTAAATCCCAAATTAACAAA
  AAGCTTTCAGAACCAGAAACAAAAAATATCTACAGTCAAAGAAAAATTGATGTGGAACCT
  GTTTTTGGATTTATGAAGGCTATTTTGGGTTTCACTCGGATGTCTGTCCGAGGACTCAAT
  AAAGTCAAAAGAGAACTTGGTTTTGTATTAATGGCACTTAATATAAGAAAAGTAGTAGCT
  CAACGAGCTGAAAATAATCAAAAAATTTATAAAAAAGACAATTTCTATATTATTTCAATA
  GAAATTGTCTTTTTTCACTTATCCAAGAACTTTATGTCCCGGACTCATTATGATTTATTT
  TTTTATTGCTGA
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⊟Protein

⊟General

• locus tag: SAUSA300_0034 [new locus tag: SAUSA300_RS00180 ]
• symbol: SAUSA300_0034
• description: IS1272, transposase
• length: 503
• theoretical pI: 9.7128
• theoretical MW: 60261.7
• GRAVY: -0.786282

⊟Function

• TIGRFAM:
• TheSEED  :
  • putative transposase
• PFAM:
  RNase_H (CL0219) DDE_Tnp_1; Transposase DDE domain (PF01609; HMM-score: 129.9)
  and 4 more
  DDE_Tnp_1_6; Transposase DDE domain (PF13751; HMM-score: 93.7)
  no clan defined DUF772; Transposase domain (DUF772) (PF05598; HMM-score: 32.3)
  UPF0179; Uncharacterised protein family (UPF0179) (PF03684; HMM-score: 10.8)
  Viral_ssRNA_CP (CL0055) Circo_capsid; Circovirus capsid protein (PF02443; HMM-score: 8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004221
  • TAT(Tat/SPI): 0.000156
  • LIPO(Sec/SPII): 0.001567
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160780 NCBI
• RefSeq: YP_492755 NCBI
• UniProt: A0A0H2XFV5 UniProt

⊟Protein sequence

• MILYAYTQSVFSGRKIEKMLNDSIRMMWLSQNQKPSYKTINQFRVNPKVDALLESLFIQFYSQCVKQNLIDDKAIFIDGTKIEANANRYTFVWKKSIQNHESKMNEDSKALYHELVTNKIIPEIKEDHDNELTKEEIDLIGSHLDKEIEDLNQHINNEKCTKTRKQIRLKRTKIKKYKKQINDYFERKYRYEFQKSILKDRNSYSKTDYDATFMRMKEDHMKNGQLKPGYNLQIATNSQFVLSYNVYQNPTDTRTMIPFLNSIQETYGHLPEYIVADAGYGSESNYKAIIDDFNRTPLITYGMFIKDKTKKYKSDIFNTQNWNYDEINDEFICPNNKRLGFKRYAYRHDKYGYKRDFKLYECDDCSECPLKNQCMNFNSKTNKKIMKNYNWEYFKSQINKKLSEPETKNIYSQRKIDVEPVFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAENNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications