PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL0042 [new locus tag: SACOL_RS00225 ]
• pan locus tag^?: SAUPAN000445000
• symbol: ccrA1
• pan gene symbol^?: ccrA
• synonym:
• product: cassette chromosome recombinase A1

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL0042 [new locus tag: SACOL_RS00225 ]
• symbol: ccrA1
• product: cassette chromosome recombinase A1
• replicon: chromosome
• strand: -
• coordinates: 47998..49347
• length: 1350
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3237009 NCBI
• RefSeq: YP_184952 NCBI
• BioCyc: see SACOL_RS00225
• MicrobesOnline: 911526 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAACAAGCAATAGGTTACTTACGACAGAGCACTACAAAGCAACAATCCTTAGCAGCA
  CAAAAACAAACCATCGAGGCATTAGCCAAAAAACATAATATTCAATACATTACCTTTTAT
  AGCGATAAGCAATCAGGACGCACTGATAAGCGGAACGGTTACCAACAAATTACTGAACTG
  ATTCAACAAGGACAATGTGATGTATTATGTTGTTACAGATTAAACCGACTTCATCGCAAT
  CTTAAAAATGCATTAAAACTCATGAAATTGTGTCAAAAATACCATGTCCATATCTTAAGC
  GTTCATGATGGCTATTTTGATATGGATAAAGCATTCGATCGGCTCAAACTCAATATTTTC
  ATCAGCTTGGCCGAACTAGAATCTGATAATATAGGCGAACAAGTCAAAAATGGAATCAAA
  GAAAAAGCGAAACAAGGTAAAATGATTACAACACATGCACCCTTTGGGTATCACTATCAT
  AATGGTACTTTCACGATAGACACAGTAAAAGCACCAACAGTAAAAGCTGTGTTCAATTAT
  TACCTTCAAGGTTATGGTTATAAAAAAATTGCGCAATACTTAGAAGCTGATGATAAATTC
  ATTAATCGTAAGCCCTATCAAGTGCGTAATATTATCCTTAACCCTAATTACTGTGGCCGT
  GTTATCAATCAATACGGACAATATGAAAACATGTTCCCAGCTATTGTCAGTACAACGATA
  TACGAAGAAGCTCAAGTTACCCGAACTCAGAAACCAGTAAAACGTAAGCCGTCAGAAAAC
  CAACTCAAACAAAAAATCAAATGTCCTTATTGTGATTCAACACTAACTAATATGACCATT
  AGAAAAAAGCACCATACATTACGTTATTATGTTTGTCCTCAAAATATGAATGCATCTCGT
  TTTGTCTGTGAATTCAAAGGGATCAACGCACAAGAATTAGAAACAAGTGTTTTAGCGACT
  TGTCAGGACTTCTTTCAAAATCAACAGCTCTATTCAAAAATAAACCATACTATTCAACAA
  CGACTCAAAAGACAAAGAGATATAGAAACTAAAACTACACTCAATCACGAGCAGCTGATA
  GAAAAATTAGCCCAAGACAAAATTGATGCAGAAACGTTCAGAGAACAAACGCAATCATTA
  CGTCAGCAATCAAAACCTATATCATCAATCAGTACGTATCAAATTCGAAAAGCTTTCCAA
  AACATCATTCAACAACGTTTCACGCTAAACATGTTGTACCCCTATATTGATGAAATTAAT
  ATTTCTAAAAATAAAAGCCTCGCTGGCATCTATTTCAAAAATGAACCACTGAATATTGTA
  AAGCAGACTATGGAATTATCAATTGTATAA
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⊟Protein

⊟General

• locus tag: SACOL0042 [new locus tag: SACOL_RS00225 ]
• symbol: CcrA1
• description: cassette chromosome recombinase A1
• length: 449
• theoretical pI: 9.93849
• theoretical MW: 52580.1
• GRAVY: -0.659688

⊟Function

• TIGRFAM:
  Hypothetical proteins Conserved conserved hypothetical protein (TIGR02328; HMM-score: 15.1)
• TheSEED  :
  • Cassette chromosome recombinase A
• PFAM:
  no clan defined Resolvase; Resolvase, N terminal domain (PF00239; HMM-score: 132)
  and 9 more
  HTH (CL0123) Recombinase; Recombinase (PF07508; HMM-score: 52.2)
  Zn_Beta_Ribbon (CL0167) Zn_ribbon_recom; Recombinase zinc beta ribbon domain (PF13408; HMM-score: 31.3)
  Zn_ribbon_PaaD; PaaD zinc beta ribbon domain (PF23451; HMM-score: 17.3)
  C2H2-zf (CL0361) zf-C2HE; C2HE / C2H2 / C2HC zinc-binding finger (PF16278; HMM-score: 13.9)
  no clan defined PyrI_C; Aspartate carbamoyltransferase regulatory chain, metal binding domain (PF02748; HMM-score: 13.3)
  Maf1; Maf1 regulator (PF09174; HMM-score: 12.8)
  TAFH; NHR1 homology to TAF (PF07531; HMM-score: 12.3)
  NTS; N-terminal segments of PfEMP1 (PF15447; HMM-score: 11.4)
  C2H2-zf (CL0361) GAGA; GAGA factor (PF09237; HMM-score: 7.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9953
  • Cytoplasmic Membrane Score: 0.0007
  • Cell wall & surface Score: 0.0007
  • Extracellular Score: 0.0033
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.03665
  • TAT(Tat/SPI): 0.002664
  • LIPO(Sec/SPII): 0.002935
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 57652411 NCBI
• RefSeq: YP_184952 NCBI
• UniProt: A0A0H2WZS7 UniProt

⊟Protein sequence

• MKQAIGYLRQSTTKQQSLAAQKQTIEALAKKHNIQYITFYSDKQSGRTDKRNGYQQITELIQQGQCDVLCCYRLNRLHRNLKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFISLAELESDNIGEQVKNGIKEKAKQGKMITTHAPFGYHYHNGTFTIDTVKAPTVKAVFNYYLQGYGYKKIAQYLEADDKFINRKPYQVRNIILNPNYCGRVINQYGQYENMFPAIVSTTIYEEAQVTRTQKPVKRKPSENQLKQKIKCPYCDSTLTNMTIRKKHHTLRYYVCPQNMNASRFVCEFKGINAQELETSVLATCQDFFQNQQLYSKINHTIQQRLKRQRDIETKTTLNHEQLIEKLAQDKIDAETFREQTQSLRQQSKPISSISTYQIRKAFQNIIQQRFTLNMLYPYIDEINISKNKSLAGIYFKNEPLNIVKQTMELSIV

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ccrB < ccrA1

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications