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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0067 [new locus tag: SACOL_RS00345 ]
  • pan locus tag?: SAUPAN000382000
  • symbol: SACOL0067
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0067 [new locus tag: SACOL_RS00345 ]
  • symbol: SACOL0067
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 76615..77547
  • length: 933
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGAAGACGTTACAGATATCGTCTTTCGACACGTTGTAAGTGAAGCAGCTAGACCGGAT
    GTGTTTTTCACTGAATTTACAAATACTGAAAGCTTTTGCCACCCTGAAGGCATACATAGT
    GTGCGCGGACGCTTAACTTTTAGTGAAGATGAACAGCCGATGGTCGCTCATATATGGGGA
    GATAAGCCAGAACAGTTCCGTGAAACGAGTATTCAATTAGCTAAAATGGGCTTTAAAGGC
    ATAGACTTAAATATGGGATGTCCTGTAGCAAATGTTGCTAAAAAGGGTAAGGGTTCCGGC
    TTAATCTTAAGACCTGACGTTGCTGCCGAAATTATTCAAGCGACTAAAGCAGGTGGGCTT
    CCGGTAAGTGTTAAAACACGCCTTGGCTACTATGAAATCGATGAATGGAAAGATTGGTTG
    AAGCACGTCTTCGAACAAGACATTGCCAATTTATCTATTCATCTTCGTACACGTAAAGAA
    ATGAGTAAAGTAGATGCACATTGGGAATTAATCGAAGCTATTAAAAATTTACGTGACGAA
    ATTGCACCAAATACATTGTTAACAATTAACGGTGATATTCCCGATAGAAAAACAGGACTT
    GAACTGGCAGAAAAATATGGCATTGATGGCGTCATGATTGGTAGAGGCATTTTCCACAAT
    CCATTCGCTTTTGAAAAAGAACCACGCGAACACACAAGCAAGGAACTATTAGATCTATTG
    AGATTGCATTTATCATTGTTTAACAAATATGAAAAAGATGAAATACGACAATTCAAGAGC
    TTGCGTAGATTCTTTAAAATCTATGTGCGTGGCATAAGAGGCGCTAGCGAACTTCGACAT
    CAATTGATGAACACACAATCAATTGCAGAAGCACGAGCACTACTCGATGAATTTGAAGCC
    CAAATGGACGAAGACGTTAAAATTGAATTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    933

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0067 [new locus tag: SACOL_RS00345 ]
  • symbol: SACOL0067
  • description: hypothetical protein
  • length: 310
  • theoretical pI: 6.14661
  • theoretical MW: 35562.5
  • GRAVY: -0.393548

⊟Function[edit | edit source]

  • reaction:
    EC 1.3.1.-?  ExPASy
    5,6-dihydrouracil in tRNA + NAD(P)+ = uracil in tRNA + NAD(P)H?
  • TIGRFAM:
    Unknown function General putative TIM-barrel protein, nifR3 family (TIGR00737; HMM-score: 152.5)
    and 3 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA dihydrouridine synthase A (TIGR00742; EC 1.-.-.-; HMM-score: 83.7)
    dihydroorotate dehydrogenase family protein (TIGR01037; EC 1.3.-.-; HMM-score: 19.3)
    Unknown function General putative TIM-barrel protein (TIGR00736; HMM-score: 15.6)
  • TheSEED  :
    • tRNA-dihydrouridine synthase DusB2
    RNA Metabolism RNA processing and modification tRNA modification Archaea  tRNA dihydrouridine synthase B (EC 1.-.-.-)
  • PFAM:
    TIM_barrel (CL0036) Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 160.8)
    and 3 more
    GT-B (CL0113) Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 16.1)
    TIM_barrel (CL0036) AP_endonuc_2; Xylose isomerase-like TIM barrel (PF01261; HMM-score: 13.8)
    no clan defined MPS2; Monopolar spindle protein 2 (PF17060; HMM-score: 12.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: FMN
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005793
    • TAT(Tat/SPI): 0.000477
    • LIPO(Sec/SPII): 0.001135
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEDVTDIVFRHVVSEAARPDVFFTEFTNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLNMGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKHVFEQDIANLSIHLRTRKEMSKVDAHWELIEAIKNLRDEIAPNTLLTINGDIPDRKTGLELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQFKSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 155 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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